Rabbit Polyclonal CCDC8 antibody. Suitable for IHC-P, ICC/IF and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human CCDC8 aa 350-500.
Images
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine)- Form
Liquid
- Clonality
Polyclonal
Immunogen
- Recombinant Fragment Protein within Human CCDC8 aa 350-500. The exact immunogen used to generate this antibody is proprietary information. Database link Q9H0W5
Reactivity data
Select an application| IHC-P | ICC/IF | |
|---|---|---|
| Human | Tested | Tested |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/200.00000 - 1/500.00000 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 0.25000-2.00000 µg/mL | Notes Fixation/Permeabilization: PFA/Triton X-100. |
Associated Products
Select an associated product type
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Target data
Function
Core component of the 3M complex, a complex required to regulate microtubule dynamics and genome integrity. It is unclear how the 3M complex regulates microtubules, it could act by controlling the level of a microtubule stabilizer (PubMed:24793695, PubMed:24793696). Required for localization of CUL7 to the centrosome (PubMed:24793695).
Alternative names
Coiled-coil domain-containing protein 8, CCDC8
Recommended products
Rabbit Polyclonal CCDC8 antibody. Suitable for IHC-P, ICC/IF and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human CCDC8 aa 350-500.
Alternative names
Coiled-coil domain-containing protein 8, CCDC8
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine)- Form
Liquid
- Clonality
Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human CCDC8 aa 350-500. The exact immunogen used to generate this antibody is proprietary information. Database link Q9H0W5
- Purification technique
Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage duration
1-2 weeks
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The CCDC8 protein known as Coiled-Coil Domain Containing 8 has a molecular mass of approximately 64 kDa. This protein is primarily expressed in the brain and other tissues where it plays a part in various cellular functions. CCDC8 is involved in processes that require structural support reflecting its high presence in complex cellular environments. Alternate names for CCDC8 include 3M2 or C3orf10 which sometimes appear in research literature.
- Biological function summary
CCDC8 interacts in the cell's cytoskeleton organization. This protein is part of the 3M complex which includes proteins such as CUL7 and OBSL1. The 3M complex is involved in maintaining cellular integrity and influencing cell cycle regulation. CCDC8 helps in achieving appropriate responses to cellular stress conditions ensuring proper cell function and survival.
- Pathways
You can find CCDC8 active in the DNA damage response and cell cycle pathways. These pathways are critical for maintaining genomic stability and CCDC8 has related interactions with proteins like TP53 a well-known tumor suppressor. CCDC8 also interacts in growth regulatory pathways linking it with other influence sensors that control cell division and differentiation.
- Associated diseases and disorders
CCDC8 mutations relate to 3M syndrome a disorder characterized by growth retardation. This syndrome links CCDC8 with proteins CUL7 and OBSL1 highlighting their combined role in skeletal development. Additionally CCDC8 has associations with cancer as its interaction with TP53 influences tumor growth regulation. Understanding these links aids studies in genetic disorders and cancer development.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
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2 product images
Immunocytochemistry/ Immunofluorescence - Anti-CCDC8 antibody (ab223784)
PFA-fixed, Triton X-100 permeabilized A431 (human epidermoid carcinoma cell line) cells stained for CCDC8 (green) using ab223784 at 4 μg/ml in ICC/IF.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CCDC8 antibody (ab223784)
Paraffin-embedded human kidney tissue stained for CCDC8 using ab223784 at 1/200 dilution in immunohistochemical analysis.
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