Rabbit Polyclonal CCDC8 antibody. Suitable for IHC-P, ICC/IF and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human Coiled-coil domain-containing protein 8 aa 350-500.
Images
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human Coiled-coil domain-containing protein 8 aa 350-500. The exact immunogen used to generate this antibody is proprietary information. Database link Q9H0W5
Reactivity data
Select an application| IHC-P | ICC/IF | |
|---|---|---|
| Human | Tested | Tested |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/200.00000 - 1/500.00000 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 0.25000-2.00000 µg/mL | Notes Fixation/Permeabilization: PFA/Triton X-100. |
Associated Products
Select an associated product type
1 product for Alternative Product
Target data
Function
Core component of the 3M complex, a complex required to regulate microtubule dynamics and genome integrity. It is unclear how the 3M complex regulates microtubules, it could act by controlling the level of a microtubule stabilizer (PubMed:24793695, PubMed:24793696). Required for localization of CUL7 to the centrosome (PubMed:24793695).
Alternative names
Coiled-coil domain-containing protein 8, CCDC8
Recommended products
Rabbit Polyclonal CCDC8 antibody. Suitable for IHC-P, ICC/IF and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human Coiled-coil domain-containing protein 8 aa 350-500.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human Coiled-coil domain-containing protein 8 aa 350-500. The exact immunogen used to generate this antibody is proprietary information. Database link Q9H0W5
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The CCDC8 protein known as Coiled-Coil Domain Containing 8 has a molecular mass of approximately 64 kDa. This protein is primarily expressed in the brain and other tissues where it plays a part in various cellular functions. CCDC8 is involved in processes that require structural support reflecting its high presence in complex cellular environments. Alternate names for CCDC8 include 3M2 or C3orf10 which sometimes appear in research literature.
- Biological function summary
CCDC8 interacts in the cell's cytoskeleton organization. This protein is part of the 3M complex which includes proteins such as CUL7 and OBSL1. The 3M complex is involved in maintaining cellular integrity and influencing cell cycle regulation. CCDC8 helps in achieving appropriate responses to cellular stress conditions ensuring proper cell function and survival.
- Pathways
You can find CCDC8 active in the DNA damage response and cell cycle pathways. These pathways are critical for maintaining genomic stability and CCDC8 has related interactions with proteins like TP53 a well-known tumor suppressor. CCDC8 also interacts in growth regulatory pathways linking it with other influence sensors that control cell division and differentiation.
- Associated diseases and disorders
CCDC8 mutations relate to 3M syndrome a disorder characterized by growth retardation. This syndrome links CCDC8 with proteins CUL7 and OBSL1 highlighting their combined role in skeletal development. Additionally CCDC8 has associations with cancer as its interaction with TP53 influences tumor growth regulation. Understanding these links aids studies in genetic disorders and cancer development.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
Immunocytochemistry/ Immunofluorescence - Anti-CCDC8 antibody (ab223784)
PFA-fixed, Triton X-100 permeabilized A431 (human epidermoid carcinoma cell line) cells stained for CCDC8 (green) using ab223784 at 4 μg/ml in ICC/IF.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CCDC8 antibody (ab223784)
Paraffin-embedded human kidney tissue stained for CCDC8 using ab223784 at 1/200 dilution in immunohistochemical analysis.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com