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AB121235

Anti-CCDC88C antibody

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(1 Publication)

Rabbit Polyclonal CCDC88C antibody. Suitable for IHC-P, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human CCDC88C aa 1550-1700.

View Alternative Names

DAPLE, KIAA1509, CCDC88C, Protein Daple, Coiled-coil domain-containing protein 88C, Dvl-associating protein with a high frequency of leucine residues, Hook-related protein 2, hDaple, HkRP2

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CCDC88C antibody (AB121235)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CCDC88C antibody (AB121235)

Immunohistochemical staining of paraffin embedded Human testis tissue, using 1/350 ab121235, shows strong nuclear positivity in cells of seminiferus ducts and Leydig cells.

Immunocytochemistry/ Immunofluorescence - Anti-CCDC88C antibody (AB121235)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-CCDC88C antibody (AB121235)

Immunofluorescent staining of Human cell line A-431 shows positivity in nucleus and nucleoli. Recommended concentration of ab121235 1-4 µg/ml. Cells treated with PFA/Triton X-100.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

ICC/IF, IHC-P

applications

Immunogen

Recombinant Fragment Protein within Human CCDC88C aa 1550-1700. The exact immunogen used to generate this antibody is proprietary information.

Q9P219

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/200 - 1/500", "IHCP-species-notes": "<p>pH 6.0</p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1-4 µg/mL", "ICCIF-species-notes": "<p>Recommend PFA Fixation and Triton X-100 treatment</p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The coiled-coil domain-containing protein 88C also known as CCDC88C or HDApL is a scaffold protein involved in intracellular signaling pathways. This protein has a molecular mass of approximately 195 kDa and is ubiquitously expressed in human tissues particularly in the brain. CCDC88C acts as a regulator of various cellular functions and possesses the ability to interact with multiple other proteins within the cell.
Biological function summary

CCDC88C facilitates organization and signaling within cellular structures. It is a part of the multiprotein complexes that regulate the cytoskeleton and influence cell polarity and migration. By interacting with other proteins CCDC88C ensures correct positioning and function of essential cellular components which is important for neurodevelopment and cellular integrity.

Pathways

CCDC88C plays a significant role in the Wnt signaling and the PI3K/Akt pathways. These pathways are vital for cell growth differentiation and survival. CCDC88C interacts with the Dishevelled (Dvl) proteins and beta-catenin within the Wnt pathway while also connecting with phosphoinositide 3-kinase (PI3K) for signaling transduction in the PI3K/Akt pathway. These interactions highlight its importance in multiple signaling cascades.

CCDC88C is linked to neurodevelopmental disorders and glioma. Mutations in CCDC88C can lead to conditions such as Intellectual Disability and Lissencephaly. In glioma it may interact with proteins such as EGFR (Epidermal Growth Factor Receptor) influencing tumor progression and response to treatments. Understanding CCDC88C's involvement in these conditions may guide therapeutic strategies.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Required for activation of guanine nucleotide-binding proteins (G-proteins) during non-canonical Wnt signaling (PubMed : 26126266). Binds to ligand-activated Wnt receptor FZD7, displacing DVL1 from the FZD7 receptor and leading to inhibition of canonical Wnt signaling (PubMed : 26126266). Acts as a non-receptor guanine nucleotide exchange factor by also binding to guanine nucleotide-binding protein G(i) alpha (Gi-alpha) subunits, leading to their activation (PubMed : 26126266). Binding to Gi-alpha subunits displaces the beta and gamma subunits from the heterotrimeric G-protein complex, triggering non-canonical Wnt responses such as activation of RAC1 and PI3K-AKT signaling (PubMed : 26126266). Promotes apical constriction of cells via ARHGEF18 (PubMed : 30948426).
See full target information CCDC88C
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Biology direct 19:13 PubMed38308285

2024

USP40 promotes hepatocellular carcinoma cell proliferation, migration and stemness by deubiquitinating and stabilizing Claudin1.

Applications

Unspecified application

Species

Unspecified reactive species

Qingsong Wu,Yuanyuan Qiu,Jinhui Guo,Zibo Yuan,Yingnan Yang,Qingwei Zhu,Zhe Zhang,Junwei Guo,Yanfang Wu,Junyu Zhang,Dongsheng Huang,Kangsheng Tu,Xiaoge Hu
View all publications
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Product promise

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For full details, please see our Terms & Conditions

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