Rabbit Polyclonal CCDC88C antibody. Suitable for IHC-P, ICC/IF and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human CCDC88C aa 1550-1700.
Images
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
Immunogen
- Recombinant Fragment Protein within Human CCDC88C aa 1550-1700. The exact immunogen used to generate this antibody is proprietary information. Database link Q9P219
Reactivity data
Select an application| IHC-P | ICC/IF | |
|---|---|---|
| Human | Tested | Tested |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/200.00000 - 1/500.00000 | Notes pH 6.0 Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1.00000-4.00000 µg/mL | Notes Recommend PFA Fixation and Triton X-100 treatment |
Target data
Function
Required for activation of guanine nucleotide-binding proteins (G-proteins) during non-canonical Wnt signaling (PubMed:26126266). Binds to ligand-activated Wnt receptor FZD7, displacing DVL1 from the FZD7 receptor and leading to inhibition of canonical Wnt signaling (PubMed:26126266). Acts as a non-receptor guanine nucleotide exchange factor by also binding to guanine nucleotide-binding protein G(i) alpha (Gi-alpha) subunits, leading to their activation (PubMed:26126266). Binding to Gi-alpha subunits displaces the beta and gamma subunits from the heterotrimeric G-protein complex, triggering non-canonical Wnt responses such as activation of RAC1 and PI3K-AKT signaling (PubMed:26126266). Promotes apical constriction of cells via ARHGEF18 (PubMed:30948426).
Alternative names
DAPLE, KIAA1509, CCDC88C, Protein Daple, Coiled-coil domain-containing protein 88C, Dvl-associating protein with a high frequency of leucine residues, Hook-related protein 2, hDaple, HkRP2
Recommended products
Rabbit Polyclonal CCDC88C antibody. Suitable for IHC-P, ICC/IF and reacts with Human samples. Immunogen corresponding to Recombinant Fragment Protein within Human CCDC88C aa 1550-1700.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine)- Form
- Liquid
- Clonality
- Polyclonal
- Immunogen
- Recombinant Fragment Protein within Human CCDC88C aa 1550-1700. The exact immunogen used to generate this antibody is proprietary information. Database link Q9P219
- Purification technique
- Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The coiled-coil domain-containing protein 88C also known as CCDC88C or HDApL is a scaffold protein involved in intracellular signaling pathways. This protein has a molecular mass of approximately 195 kDa and is ubiquitously expressed in human tissues particularly in the brain. CCDC88C acts as a regulator of various cellular functions and possesses the ability to interact with multiple other proteins within the cell.
- Biological function summary
CCDC88C facilitates organization and signaling within cellular structures. It is a part of the multiprotein complexes that regulate the cytoskeleton and influence cell polarity and migration. By interacting with other proteins CCDC88C ensures correct positioning and function of essential cellular components which is important for neurodevelopment and cellular integrity.
- Pathways
CCDC88C plays a significant role in the Wnt signaling and the PI3K/Akt pathways. These pathways are vital for cell growth differentiation and survival. CCDC88C interacts with the Dishevelled (Dvl) proteins and beta-catenin within the Wnt pathway while also connecting with phosphoinositide 3-kinase (PI3K) for signaling transduction in the PI3K/Akt pathway. These interactions highlight its importance in multiple signaling cascades.
- Associated diseases and disorders
CCDC88C is linked to neurodevelopmental disorders and glioma. Mutations in CCDC88C can lead to conditions such as Intellectual Disability and Lissencephaly. In glioma it may interact with proteins such as EGFR (Epidermal Growth Factor Receptor) influencing tumor progression and response to treatments. Understanding CCDC88C's involvement in these conditions may guide therapeutic strategies.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
Immunocytochemistry/ Immunofluorescence - Anti-CCDC88C antibody (ab121235)
Immunofluorescent staining of Human cell line A-431 shows positivity in nucleus and nucleoli. Recommended concentration of ab121235 1-4 µg/ml. Cells treated with PFA/Triton X-100.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CCDC88C antibody (ab121235)
Immunohistochemical staining of paraffin embedded Human testis tissue, using 1/350 ab121235, shows strong nuclear positivity in cells of seminiferus ducts and Leydig cells.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com