Rabbit Polyclonal CCDC97 antibody. Suitable for IP, WB and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human CCDC97 aa 250 to C-terminus.
Images
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7 - 8
Preservative: 0.09% Sodium azide
Constituents: Tris citrate/phosphate- Form
Liquid
- Clonality
Polyclonal
Immunogen
- Synthetic Peptide within Human CCDC97 aa 250 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q96F63
Reactivity data
Select an application| IP | WB | |
|---|---|---|
| Human | Tested | Tested |
| Chimpanzee | Predicted | Predicted |
| Cow | Predicted | Predicted |
| Cynomolgus monkey | Predicted | Predicted |
| Dog | Predicted | Predicted |
| Horse | Predicted | Predicted |
| Orangutan | Predicted | Predicted |
| Pig | Predicted | Predicted |
| Rhesus monkey | Predicted | Predicted |
| Sheep | Predicted | Predicted |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 2.00000-10.00000 µg/mg of lysate | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Sheep, Horse, Cow, Dog, Pig, Chimpanzee, Cynomolgus monkey, Rhesus monkey, Orangutan | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/2000.00000 - 1/10000.00000 | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Sheep, Horse, Cow, Dog, Pig, Chimpanzee, Cynomolgus monkey, Rhesus monkey, Orangutan | Dilution info - | Notes - |
Alternative names
Coiled-coil domain-containing protein 97, CCDC97
Recommended products
Rabbit Polyclonal CCDC97 antibody. Suitable for IP, WB and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human CCDC97 aa 250 to C-terminus.
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7 - 8
Preservative: 0.09% Sodium azide
Constituents: Tris citrate/phosphate- Form
Liquid
- Clonality
Polyclonal
- Immunogen
- Synthetic Peptide within Human CCDC97 aa 250 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link Q96F63
- Purification technique
Affinity purification Immunogen
- Concentration
- Purification notes
ab225997 was affinity purified using an epitope specific to CCDC97 immobilized on solid support.
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage duration
1-2 weeks
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
CCDC97 also known as Coiled-Coil Domain Containing 97 functions as a structural protein that contributes to the proper assembly of cellular structures. This protein has an approximate mass of 52 kDa and is widely expressed in various tissues with higher levels found in the testis and brain. CCDC97 participates in the organization of microtubules supporting specific cellular functions and maintaining the integrity of structural frameworks.
- Biological function summary
CCDC97 plays a critical role in cell division and stability. It is part of the KMN network complex which is important for kinetochore function during mitosis. The KMN network ensures accurate chromosome segregation preventing aneuploidy by securing the attachment of spindle microtubules. CCDC97 contributes to stabilizing the complex and interacts with other proteins to ensure precise cellular replication processes.
- Pathways
The activities of CCDC97 relate closely to the spindle assembly checkpoint and cell cycle regulation. These pathways involve proteins like Aurora B and MAD1 which control proper chromosome alignment and attachment to the spindle apparatus. Through its inclusion in the KMN network CCDC97 influences these pathways by interacting directly with essential partners to guarantee checkpoint activation and chromosomal stability throughout cell division.
- Associated diseases and disorders
Irregularities in CCDC97 expression or function may contribute to cancer and neurodegenerative diseases. Aberrant chromosome segregation influenced by CCDC97 malfunctions can result in aneuploidy a hallmark of various cancers. The protein also potentially relates to Alzheimer's Disease through stress-related alterations in microtubule dynamics involving proteins like tau in the neuronal cytoskeleton. These connections highlight the significance of CCDC97 in maintaining cellular health and its potential role in disease development.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
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2 product images
Western blot - Anti-CCDC97 antibody (ab225997)
All lanes: Western blot - Anti-CCDC97 antibody (ab225997) at 0.1 µg/mL
Lane 1: HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg
Lane 2: HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg
Lane 3: Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 39 kDa
Exposure time: 30s
Immunoprecipitation - Anti-CCDC97 antibody (ab225997)
CCDC97 was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab225997 at 6 μg/mg lysate. Western blot was performed from the immunoprecipitate using ab225997 at 1 μg/ml.
Lane 1: ab225997 IP in HEK-293T whole cell lysate.
Lane 2: Control IgG IP in HEK-293T whole cell lysate.
Detection: Chemiluminescence with exposure time of 30 seconds.
All lanes: Immunoprecipitation - Anti-CCDC97 antibody (ab225997)
Predicted band size: 39 kDa
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