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Rabbit Recombinant Monoclonal CCDC98 antibody. Carrier free. Suitable for ICC/IF, IP, WB, Flow Cyt (Intra) and reacts with Human samples.

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Images

Western blot - Anti-CCDC98 antibody [EPR6310(2)] - BSA and Azide free (AB248872), expandable thumbnail
  • Flow Cytometry (Intracellular) - Anti-CCDC98 antibody [EPR6310(2)] - BSA and Azide free (AB248872), expandable thumbnail
  • Immunoprecipitation - Anti-CCDC98 antibody [EPR6310(2)] - BSA and Azide free (AB248872), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-CCDC98 antibody [EPR6310(2)] - BSA and Azide free (AB248872), expandable thumbnail

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Constituents: PBS

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
ICC/IFIPWBIHC-PFlow Cyt (Intra)
Human
Tested
Tested
Tested
Not recommended
Tested

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human
Dilution info
-
Notes

-

Tested
Tested

Species
Human
Dilution info
-
Notes

-

Target data

Function

Involved in DNA damage response and double-strand break (DSB) repair. Component of the BRCA1-A complex, acting as a central scaffold protein that assembles the various components of the complex and mediates the recruitment of BRCA1. The BRCA1-A complex specifically recognizes 'Lys-63'-linked ubiquitinated histones H2A and H2AX at DNA lesion sites, leading to target the BRCA1-BARD1 heterodimer to sites of DNA damage at DSBs. This complex also possesses deubiquitinase activity that specifically removes 'Lys-63'-linked ubiquitin on histones H2A and H2AX.

Alternative names

ABRA1, CCDC98, FAM175A, UNQ496/PRO1013, ABRAXAS1, BRCA1-A complex subunit Abraxas 1, Coiled-coil domain-containing protein 98, Protein FAM175A

Recommended products

Rabbit Recombinant Monoclonal CCDC98 antibody. Carrier free. Suitable for ICC/IF, IP, WB, Flow Cyt (Intra) and reacts with Human samples.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Carrier free
Yes
Clone number
EPR6310(2)
Purification technique
Affinity purification
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze

Notes

ab248872 is the carrier-free version of Anti-CCDC98 antibody [EPR6310(2)] ab139191.

Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.

This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

CCDC98 also known as NABP2 or MERIT40 functions as a protein that assists in the DNA damage response and repair pathways. Its molecular mass is approximately 42 kDa. CCDC98 primarily shows expression in the nucleus of cells. It is essential in repair processes by interacting with other proteins involved in the DNA double-strand break repair mechanism. The protein ensures stability within the BRCA1-A complex playing a role in maintaining genomic integrity.

Biological function summary

CCDC98 supports cellular processes by stabilizing the BRCA1-A complex which is important for the homologous recombination repair of damaged DNA. This protein forms an important part of the machinery that recognizes and processes DNA damage. It interacts with other core components of the DNA repair system like BRCA1 RAP80 and ABRAXAS ensuring efficient repair and prevention of genomic instability.

Pathways

CCDC98 is integral to the homologous recombination repair and cell cycle checkpoint pathways. Through its association with the BRCA1-A complex CCDC98 influences the recruitment and retention of repair proteins at DNA damage sites a critical event in the DNA damage response pathway. It connects with other proteins such as BRCA1 and RAP80 to regulate repair pathway activities and proper cell cycle progression reinforcing cellular responses to DNA damage.

Associated diseases and disorders

CCDC98 is linked to breast and ovarian cancers. Mutations or dysregulation affecting CCDC98's function can disrupt BRCA1-A complex stability leading to compromised DNA repair ability which is a factor in tumorigenesis. It connects to the BRCA1 protein in these disease contexts where impaired interactions can raise the risk of developing cancer showcasing the significance of this protein in health and disease contexts.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

4 product images

  • Western blot - Anti-CCDC98 antibody [EPR6310(2)] - BSA and Azide free (ab248872), expandable thumbnail

    Western blot - Anti-CCDC98 antibody [EPR6310(2)] - BSA and Azide free (ab248872)

    This data was developed using Anti-CCDC98 antibody [EPR6310(2)] ab139191, the same antibody clone in a different buffer formulation.

    All lanes: Western blot - Anti-CCDC98 antibody [EPR6310(2)] (Anti-CCDC98 antibody [EPR6310(2)] ab139191) at 1/1000 dilution

    Lane 1: HeLa cell lysate at 10 µg

    Lane 2: MCF7 cell lysate at 10 µg

    Lane 3: 293T (Human embryonic kidney epithelial cell) cell lysate at 10 µg

    Lane 4: Jurkat cell lysate at 10 µg

    Secondary

    All lanes: Goat anti-rabbit HRP at 1/2000 dilution

    Predicted band size: 47 kDa

  • Flow Cytometry (Intracellular) - Anti-CCDC98 antibody [EPR6310(2)] - BSA and Azide free (ab248872), expandable thumbnail

    Flow Cytometry (Intracellular) - Anti-CCDC98 antibody [EPR6310(2)] - BSA and Azide free (ab248872)

    This data was developed using Anti-CCDC98 antibody [EPR6310(2)] ab139191, the same antibody clone in a different buffer formulation.Overlay histogram showing HeLa cells stained with Anti-CCDC98 antibody [EPR6310(2)] ab139191 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (Anti-CCDC98 antibody [EPR6310(2)] ab139191, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1?g/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

  • Immunoprecipitation - Anti-CCDC98 antibody [EPR6310(2)] - BSA and Azide free (ab248872), expandable thumbnail

    Immunoprecipitation - Anti-CCDC98 antibody [EPR6310(2)] - BSA and Azide free (ab248872)

    This data was developed using Anti-CCDC98 antibody [EPR6310(2)] ab139191, the same antibody clone in a different buffer formulation.CCDC98 was immunoprecipitated from 0.35 mg HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate 10 µg with Anti-CCDC98 antibody [EPR6310(2)] ab139191 at 1/60 dilution (2µg). VeriBlot for IP Detection Reagent (HRP)(VeriBlot for IP Detection Reagent (HRP) ab131366) was used at 1/5000 dilution.

    Lane 1: HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate 10 µg

    Lane 2: abab139191 IP in HeLa whole cell lysate

    Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-CCDC98 antibody [EPR6310(2)] ab139191 in HeLa whole cell lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    All lanes: Immunoprecipitation - Anti-CCDC98 antibody [EPR6310(2)] (Anti-CCDC98 antibody [EPR6310(2)] ab139191)

    Predicted band size: 47 kDa

    Observed band size: 47 kDa

  • Immunocytochemistry/ Immunofluorescence - Anti-CCDC98 antibody [EPR6310(2)] - BSA and Azide free (ab248872), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-CCDC98 antibody [EPR6310(2)] - BSA and Azide free (ab248872)

    This data was developed using Anti-CCDC98 antibody [EPR6310(2)] ab139191, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of HeLa cells labelling CCDC98 with Anti-CCDC98 antibody [EPR6310(2)] ab139191 at 1/100 dilution.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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