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AB248872

Anti-CCDC98 antibody [EPR6310(2)] - BSA and Azide free

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(1 Publication)

Rabbit Recombinant Monoclonal CCDC98 antibody. Carrier free. Suitable for ICC/IF, IP, WB, Flow Cyt (Intra) and reacts with Human samples. Cited in 1 publication.

View Alternative Names

ABRA1, CCDC98, FAM175A, UNQ496/PRO1013, ABRAXAS1, BRCA1-A complex subunit Abraxas 1, Coiled-coil domain-containing protein 98, Protein FAM175A

4 Images
Immunocytochemistry/ Immunofluorescence - Anti-CCDC98 antibody [EPR6310(2)] - BSA and Azide free (AB248872)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-CCDC98 antibody [EPR6310(2)] - BSA and Azide free (AB248872)

This data was developed using ab139191, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of HeLa cells labelling CCDC98 with ab139191 at 1/100 dilution.

Flow Cytometry (Intracellular) - Anti-CCDC98 antibody [EPR6310(2)] - BSA and Azide free (AB248872)
  • Flow Cyt (Intra)

Unknown

Flow Cytometry (Intracellular) - Anti-CCDC98 antibody [EPR6310(2)] - BSA and Azide free (AB248872)

This data was developed using ab139191, the same antibody clone in a different buffer formulation.

Overlay histogram showing HeLa cells stained with ab139191 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab139191, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1µg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

Immunoprecipitation - Anti-CCDC98 antibody [EPR6310(2)] - BSA and Azide free (AB248872)
  • IP

Lab

Immunoprecipitation - Anti-CCDC98 antibody [EPR6310(2)] - BSA and Azide free (AB248872)

This data was developed using ab139191, the same antibody clone in a different buffer formulation.

CCDC98 was immunoprecipitated from 0.35 mg HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate 10 μg with ab139191 at 1/60 dilution (2μg). VeriBlot for IP Detection Reagent (HRP) (ab131366) was used at 1/5000 dilution.

Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate 10 μg

Lane 2 : ab139191 IP in HeLa whole cell lysate

Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab139191 in HeLa whole cell lysate

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

All lanes:

Immunoprecipitation - Anti-CCDC98 antibody [EPR6310(2)] (<a href='/en-us/products/primary-antibodies/ccdc98-antibody-epr63102-ab139191'>ab139191</a>)

Predicted band size: 47 kDa

Observed band size: 47 kDa

false

Western blot - Anti-CCDC98 antibody [EPR6310(2)] - BSA and Azide free (AB248872)
  • WB

Unknown

Western blot - Anti-CCDC98 antibody [EPR6310(2)] - BSA and Azide free (AB248872)

This data was developed using ab139191, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-CCDC98 antibody [EPR6310(2)] (<a href='/en-us/products/primary-antibodies/ccdc98-antibody-epr63102-ab139191'>ab139191</a>) at 1/1000 dilution

Lane 1:

HeLa cell lysate at 10 µg

Lane 2:

MCF7 cell lysate at 10 µg

Lane 3:

293T (Human embryonic kidney epithelial cell) cell lysate at 10 µg

Lane 4:

Jurkat cell lysate at 10 µg

Secondary

All lanes:

Goat anti-rabbit HRP at 1/2000 dilution

Predicted band size: 47 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR6310(2)

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

Flow Cyt (Intra), WB, IP, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "<p></p>" } } }
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Product details

ab248872 is the carrier-free version of ab139191.

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CCDC98 also known as NABP2 or MERIT40 functions as a protein that assists in the DNA damage response and repair pathways. Its molecular mass is approximately 42 kDa. CCDC98 primarily shows expression in the nucleus of cells. It is essential in repair processes by interacting with other proteins involved in the DNA double-strand break repair mechanism. The protein ensures stability within the BRCA1-A complex playing a role in maintaining genomic integrity.
Biological function summary

CCDC98 supports cellular processes by stabilizing the BRCA1-A complex which is important for the homologous recombination repair of damaged DNA. This protein forms an important part of the machinery that recognizes and processes DNA damage. It interacts with other core components of the DNA repair system like BRCA1 RAP80 and ABRAXAS ensuring efficient repair and prevention of genomic instability.

Pathways

CCDC98 is integral to the homologous recombination repair and cell cycle checkpoint pathways. Through its association with the BRCA1-A complex CCDC98 influences the recruitment and retention of repair proteins at DNA damage sites a critical event in the DNA damage response pathway. It connects with other proteins such as BRCA1 and RAP80 to regulate repair pathway activities and proper cell cycle progression reinforcing cellular responses to DNA damage.

CCDC98 is linked to breast and ovarian cancers. Mutations or dysregulation affecting CCDC98's function can disrupt BRCA1-A complex stability leading to compromised DNA repair ability which is a factor in tumorigenesis. It connects to the BRCA1 protein in these disease contexts where impaired interactions can raise the risk of developing cancer showcasing the significance of this protein in health and disease contexts.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Involved in DNA damage response and double-strand break (DSB) repair. Component of the BRCA1-A complex, acting as a central scaffold protein that assembles the various components of the complex and mediates the recruitment of BRCA1. The BRCA1-A complex specifically recognizes 'Lys-63'-linked ubiquitinated histones H2A and H2AX at DNA lesion sites, leading to target the BRCA1-BARD1 heterodimer to sites of DNA damage at DSBs. This complex also possesses deubiquitinase activity that specifically removes 'Lys-63'-linked ubiquitin on histones H2A and H2AX.
See full target information ABRAXAS1
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Journal of personalized medicine 12: PubMed35207643

2022

Detyrosinated α-Tubulin, Vimentin and PD-L1 in Circulating Tumor Cells (CTCs) Isolated from Non-Small Cell Lung Cancer (NSCLC) Patients.

Applications

Unspecified application

Species

Unspecified reactive species

Spyridoula D Katsarou,Ippokratis Messaritakis,Anastasia Voumvouraki,Stavros Kakavogiannis,Athanasios Κotsakis,Saad Alkahtani,Christos Stournaras,Stuart S Martin,Vassilis Georgoulias,Galatea Kallergi
View all publications
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