AB77077

Anti-CCK2-R antibody

Name: Anti-CCK2-R antibody (ab77077) | Abcam
Brand: Abcam Limited
SKU: AB77077
Price: 535 USD
Availability: InStock
Rating: 4 (2 reviews)

(2 Reviews)

(11 Publications)

Goat Polyclonal CCK2-R antibody. Suitable for WB, IHC-P and reacts with Mouse, Human samples. Cited in 11 publications. Immunogen corresponding to Synthetic Peptide within Human CCKBR aa 250-300.

View Alternative Names

CCKRB, CCKBR, Gastrin/cholecystokinin type B receptor, CCK-B receptor, CCK-BR, Cholecystokinin-2 receptor, CCK2-R

1 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CCK2-R antibody (AB77077)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CCK2-R antibody (AB77077)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human pancreas tissue labeling CCK2-R with ab77077 at 6µg/mL. Negative Control : no primary antibody. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.

Key facts

Host species

Goat

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Mouse, Human

Applications

WB, IHC-P

applications

Immunogen

Synthetic Peptide within Human CCKBR aa 250-300. The exact immunogen used to generate this antibody is proprietary information.

P32239

style

left-column

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "4-6 µg/mL", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval via the microwave method before commencing with IHC staining protocol." }, "Mouse": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "0.5 µg/mL", "WB-species-notes": "<p></p>", "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "predicted", "IHCP-species-dilution-info": "", "IHCP-species-notes": "" } } }

style

left-column

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Immunogen

Purification notes

ab77077 is purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunising peptide.

Storage buffer

pH: 7.3 Preservative: 0.02% Sodium azide Constituents: 99% Tris buffered saline, 0.5% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CCK2 receptors also known as cholecystokinin B receptors play important roles in the body. These receptors are part of the G protein-coupled receptor family. They have a molecular mass of approximately 48 kDa. CCK2 receptors mainly express in the brain and gastric mucosa. In the brain they regulate neurotransmission while in the stomach they influence gastric acid secretion.

Biological function summary

CCK2 receptors interact with cholecystokinin and gastrin hormones. They are essential components of signaling complexes that mediate their effects. Interaction with these hormones leads to increased cellular calcium levels. This receptor also plays a role in modulating anxiety and pain perception in addition to its role in digestive processes.

Pathways

CCK2 receptors are key in the gastrin signaling pathway and influence the phosphoinositide pathway. These pathways involve interaction with other proteins like CCK1 receptors and tyrosine kinases. The interplay with these pathways contributes to the activation of intracellular signaling cascades that affect cell growth and digestive enzyme secretion.

CCK2 receptors have links to anxiety disorders and gastric cancers. Alterations in receptor function can influence the brain's response to stress linking it to anxiety. For gastric cancers overexpression of CCK2 receptors can lead to increased cell proliferation. Their interaction with phosphoinositide 3-kinase also plays a role in the progression of gastric cancers.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

Receptor for the peptide hormones gastrin and cholecystokinin (CCK). Expressed throughout the central nervous system, where it modulates processes such as anxiety, analgesia, arousal and neuroleptic activity. Couples to both GNAI1 and GNAQ signaling pathways, but not to GNAS (PubMed : 34556863). Upon gastrin activation, reduces glucose absorption in intestinal epithelial cells by downregulating SGLT1 and GLUT2 expression through suppression of the PI3K/Akt/eIF4B pathway (By similarity). In the kidney, decreases SGLT2 expression under high-glucose conditions via ERK/NF-kappa-B signaling (By similarity).. Isoform 2 is constitutively activated and may regulate cancer cell proliferation via a gastrin-independent mechanism.

See full target information CCKBR

style

left-column

Publications (11)

Recent publications for all applications. Explore the full list and refine your search

eNeuro 12: PubMed39809536

2025

Cortical HFS-Induced Neo-Hebbian Local Plasticity Enhances Efferent Output Signal and Strengthens Afferent Input Connectivity.

Applications

Unspecified application

Species

Unspecified reactive species

Xiao Li,Xue Wang,Xiaohan Hu,Peng Tang,Congping Chen,Ling He,Mengying Chen,Stephen Temitayo Bello,Tao Chen,Xiaoyu Wang,Yin Ting Wong,Wenjian Sun,Xi Chen,Jianan Qu,Jufang He

iScience 26:106542 PubMed37123227

2023

Interhemispheric cortical long-term potentiation in the auditory cortex requires heterosynaptic activation of entorhinal projection.

Applications

Unspecified application

Species

Unspecified reactive species

Xiao Li,Ling He,Xiaohan Hu,Fengwen Huang,Xue Wang,Mengying Chen,Ezra Ginn Yoon,Stephen Temitayo Bello,Tao Chen,Xi Chen,Peng Tang,Congping Chen,Jianan Qu,Jufang He

Frontiers in oncology 11:788875 PubMed34926305

2021

Gastrin Vaccine Alone and in Combination With an Immune Checkpoint Antibody Inhibits Growth and Metastases of Gastric Cancer.

Applications

Unspecified application

Species

Unspecified reactive species

Jill P Smith,Hong Cao,Wenqiang Chen,Kanwal Mahmood,Teresa Phillips,Lynda Sutton,Allen Cato

Biomolecules 11: PubMed34944412

2021

Cholecystokinin-B Receptor-Targeted Nanoparticle for Imaging and Detection of Precancerous Lesions in the Pancreas.

Applications

Unspecified application

Species

Unspecified reactive species

Jill P Smith,Hong Cao,Elijah F Edmondson,Siva Sai Krishna Dasa,Stephan T Stern

Nature communications 11:5692 PubMed33173030

2020

Distinct CCK-positive SFO neurons are involved in persistent or transient suppression of water intake.

Applications

Unspecified application

Species

Unspecified reactive species

Takashi Matsuda,Takeshi Y Hiyama,Kenta Kobayashi,Kazuto Kobayashi,Masaharu Noda

Theranostics 10:10861-10873 PubMed33042258

2020

Pharmacological inhibition of mTORC1 increases CCKBR-specific tumor uptake of radiolabeled minigastrin analogue [Lu]Lu-PP-F11N.

Applications

Unspecified application

Species

Unspecified reactive species

Michal Grzmil,Yun Qin,Carina Schleuniger,Stephan Frank,Stefan Imobersteg,Alain Blanc,Martin Spillmann,Philipp Berger,Roger Schibli,Martin Behe

American journal of physiology. Gastrointestinal a 317:G682-G693 PubMed31433212

2019

Vaccine against gastrin, a polyclonal antibody stimulator, decreases pancreatic cancer metastases.

Applications

Unspecified application

Species

Unspecified reactive species

Nicholas Osborne,Rebecca Sundseth,Martha D Gay,Hong Cao,Robin D Tucker,Sandeep Nadella,Shangzi Wang,Xunxian Liu,Alexander Kroemer,Lynda Sutton,Allen Cato,Jill P Smith

Cancer immunology, immunotherapy : CII 67:195-207 PubMed29043413

2017

Cholecystokinin receptor antagonist alters pancreatic cancer microenvironment and increases efficacy of immune checkpoint antibody therapy in mice.

Applications

ICC/IF

Species

Unspecified reactive species

Jill P Smith,Shangzi Wang,Sandeep Nadella,Sandra A Jablonski,Louis M Weiner

Pancreas 43:1050-9 PubMed25058882

2014

Cholecystokinin receptor antagonist halts progression of pancreatic cancer precursor lesions and fibrosis in mice.

Applications

Unspecified application

Species

Unspecified reactive species

Jill P Smith,Timothy K Cooper,Christopher O McGovern,Evan L Gilius,Qing Zhong,Jiangang Liao,Alfredo A Molinolo,J Silvio Gutkind,Gail L Matters

Proceedings of the National Academy of Sciences of 109:21295-300 PubMed23236171

2012

Heterobivalent ligands target cell-surface receptor combinations in vivo.

Applications

IHC-Fr

Species

Unspecified reactive species

Liping Xu,Jatinder S Josan,Josef Vagner,Michael R Caplan,Victor J Hruby,Eugene A Mash,Ronald M Lynch,David L Morse,Robert J Gillies

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-CCK2-R antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CCK2-R antibody (AB77077)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Complementary Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (11)

Product promise