Rabbit Polyclonal CCK4 antibody. Suitable for IP, WB and reacts with Human, Mouse samples. Cited in 2 publications. Immunogen corresponding to Synthetic Peptide within Human PTK7 aa 700-750.
View Alternative Names
CCK4, PTK7, Inactive tyrosine-protein kinase 7, Colon carcinoma kinase 4, Protein-tyrosine kinase 7, Pseudo tyrosine kinase receptor 7, Tyrosine-protein kinase-like 7, CCK-4
- IP
Supplier Data
Immunoprecipitation - Anti-CCK4 antibody (AB225874)
CCK4 was immunoprecipitated from Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate (prepared using NETN buffer; 1 mg for IP, 20% of IP loaded) with ab225874 at 6 μg/mg lysate. Western blot was performed from the immunoprecipitate using ab225874 at 1 μg/ml.
Lane 1 : ab225874 IP in Jurkat whole cell lysate.
Lane 2 : Control IgG IP in Jurkat whole cell lysate.
Detection : Chemiluminescence with exposure time of 30 seconds.
All lanes:
Immunoprecipitation - Anti-CCK4 antibody (ab225874)
Predicted band size: 118 kDa
false
- WB
Supplier Data
Western blot - Anti-CCK4 antibody (AB225874)
Lysates prepared using NETN buffer.
All lanes:
Western blot - Anti-CCK4 antibody (ab225874) at 0.1 µg/mL
Lane 1:
HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg
Lane 2:
HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg
Lane 3:
Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate at 50 µg
Lane 4:
TCMK-1 (mouse kidney epithelial cell line) whole cell lysate at 50 µg
Lane 5:
NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysate at 50 µg
Predicted band size: 118 kDa
true
Exposure time: 3min
Alternative Products
Complementary Products
Primary Antibodies
AB272235
Anti-TCF-4/TCF7L2+TCF-3/TCF7L1 antibody [EPR24701-87]
20ul selling size|RabMAb|Recombinant|Trial Size
![Western blot - Anti-TCF-4/TCF7L2+TCF-3/TCF7L1 antibody [EPR24701-87] (AB272235)](https://content.abcam.com/products/images/tcf-4-tcf7l2tcf-3-tcf7l1-antibody-epr24701-87-ab272235--western-blot-img169665.jpg)
- 0
- 0
Reactivity data
Properties and storage information
Form
Purification technique
Purification notes
Storage buffer
Shipped at conditions
Appropriate short-term storage duration
Appropriate short-term storage conditions
Appropriate long-term storage conditions
Aliquoting information
Storage information
Supplementary information
This supplementary information is collated from multiple sources and compiled automatically.
Biological function summary
CCK4 participates in cellular signaling involved in adhesion and cell migration. It localizes predominantly in the membranes participating in complex cellular environments such as the Wnt signaling complex. This suggests a role in maintaining cellular architecture and signaling pathways that influence developmental processes. Its interactions within these complexes can help maintain balance and functional integrity in developing tissues.
Pathways
CCK4 engages in vital pathways influencing cell adhesion and movement including the planar cell polarity (PCP) pathway and Wnt signaling pathway. Within these pathways CCK4 interacts with proteins such as Dishevelled and Frizzled establishing its role in guiding cellular and developmental patterns. These interactions regulate important aspects of cell polarity and directional movement during embryonic development.
Product protocols
- Visit the General protocols
- Visit the Troubleshooting
Target data
Publications (2)
Recent publications for all applications. Explore the full list and refine your search
Thoracic cancer 16:e15485 PubMed39552193
2024
Applications
Unspecified application
Species
Unspecified reactive species
Cell reports. Medicine 4:100953 PubMed36809766
2023
Applications
Unspecified application
Species
Unspecified reactive species
Product promise
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com