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AB272382

Anti-CCL14 antibody [EPR22872-43] - BSA and Azide free (Capture)

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Rabbit Recombinant Monoclonal CCL14 antibody. Carrier free. Suitable for sELISA and reacts with Human samples.

View Alternative Names

NCC2, SCYA14, CCL14, C-C motif chemokine 14, Chemokine CC-1/CC-3, HCC-1(1-74), NCC-2, Small-inducible cytokine A14, HCC-1/HCC-3

1 Images
Sandwich ELISA - Anti-CCL14 antibody [EPR22872-43] - BSA and Azide free (Capture) (AB272382)
  • sELISA

Supplier Data

Sandwich ELISA - Anti-CCL14 antibody [EPR22872-43] - BSA and Azide free (Capture) (AB272382)

Representative standard curve from corresponding SimpleStep ELISA® Kit (ab272201).

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR22872-43

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

sELISA

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "sELISA" : {"fullname" : "Sandwich ELISA", "shortname":"sELISA"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "sELISA-species-checked": "guaranteed", "sELISA-species-dilution-info": "", "sELISA-species-notes": "<p></p>" } } }
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Product details

ab272382 is a BSA and Azide Free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Human CCL14. The recommended pair for sandwich ELISA is:
Capture: ab272382, Human CCL14 Capture Antibody (unconjugated)
Detector: ab272383, Human CCL14 Detector Antibody (unconjugated)
The reference range value is 5.469 - 350 pg/ml.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Sandwich ELISA
The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CCL14 also known as HCC-1 or HCC-3 is a small cytokine belonging to the CC chemokine family. It has a molecular weight of approximately 8 kDa. The protein is found in a variety of tissues where it is secreted by immune cells fibroblasts and endothelial cells. CCL14 interacts with chemokine receptors to mediate cell signaling and can be detected at the interface of inflammatory responses. Its expression can be modulated during different immune responses.
Biological function summary

The CCL14 protein acts as a chemoattractant for specific types of white blood cells including monocytes and lymphocytes which are critical in mounting immune responses. It does not function as a part of a larger complex but operates independently to influence immune cell trafficking. CCL14 is involved in modulating inflammatory reactions by recruiting immune cells to sites of tissue damage or infection. It helps orchestrate a coordinated immune response by activating target cells through its receptor binding.

Pathways

CCL14 plays important roles within the immune response and inflammation pathways. It contributes to the chemokine signaling pathway facilitating the migration and activation of leukocytes. Another significant pathway involving CCL14 is the MAPK signaling pathway which it influences through interactions with other signaling molecules such as CCL3 and CCL5. These relationships highlight CCL14's roles in both promoting and controlling immune responses.

CCL14 is linked to various inflammatory and immune-related diseases. It shows a strong association with chronic inflammation disorders such as rheumatoid arthritis where it contributes to tissue infiltration by immune cells. CCL14 also plays a role in atherosclerosis where it can influence the recruitment of macrophages that contribute to plaque formation. Through these conditions CCL14 interacts with other proteins involved in these pathologies including CCL2 and CXCL8 which further modulate inflammatory processes.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Has weak activities on human monocytes and acts via receptors that also recognize MIP-1 alpha. It induces intracellular Ca(2+) changes and enzyme release, but no chemotaxis, at concentrations of 100-1,000 nM, and is inactive on T-lymphocytes, neutrophils, and eosinophil leukocytes. Enhances the proliferation of CD34 myeloid progenitor cells. The processed form HCC-1(9-74) is a chemotactic factor that attracts monocytes, eosinophils, and T-cells and is a ligand for CCR1, CCR3 and CCR5.
See full target information CCL14
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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