Anti-CCL18 antibody [EPR24781-84] (BSA and Azide free)

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CCL18 antibody [EPR24781-84] (BSA and Azide free) (AB300058)

This data was developed using ab300057, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labelling CCL18 with ab300057 at 1/500 (0.982 μg/ml) followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer). Positive staining on human tonsil. The section was incubated with ab300057 at 4°C overnight. Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CCL18 antibody [EPR24781-84] (BSA and Azide free) (AB300058)

This data was developed using ab300057, the same antibody clone in a different buffer formulation.

Immunohistochemical analysis of paraffin-embedded Human stomach cancer tissue labelling CCL18 with ab300057 at 1/500 (0.982 μ/ml) followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer). Positive staining on human stomach cancer. The section was incubated with ab300057 at 4°C overnight. Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CCL18 antibody [EPR24781-84] (BSA and Azide free) (AB300058)

This data was developed using ab300057, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded A) HEK-293T cells transfected with human C-C motif chemokine 18 expression vector containing a myc-his tag, B) HEK-293T cells untreated labelling CCL18 with ab300057 at 1/200 (2.455 µg/ml) followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer). Cytoplasmic staining on HEK-293T cells transfected with a human C-C motif chemokine 18 expression vector containing a myc-His tag (image A). No staining on HEK-293T cells untreated (image B). The section was incubated with ab300057 at 4°C overnight. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CCL18 antibody [EPR24781-84] (BSA and Azide free) (AB300058)

This data was developed using ab300057, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labelling CCL18 with ab300057 at 1/500 (0.982 µg/ml) followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer). Positive staining on human tonsil. The section was incubated with ab300057 at 4°C overnight. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

• WB

Supplier Data

Western blot - Anti-CCL18 antibody [EPR24781-84] (BSA and Azide free) (AB300058)

This data was developed using ab300057, the same antibody clone in a different buffer formulation.  5% NFDM/TBST was used as blocking and diluting buffer and concentration.  THP-1 cells after PMA and IL-4 treatment secrete high levels of CCL18. (PMID : 25502147).

All lanes:

Western blot - Anti-CCL18 antibody [EPR24781-84] (<a href='/en-us/products/primary-antibodies/ccl18-antibody-epr24781-84-ab300057'>ab300057</a>) at 1/1000 dilution

Lane 1:

THP-1( Human monocytic leukemia monocyte) treated with 0.1µg/ml PMA for 48 hours whole cell lysate at 50 µg

Lane 2:

THP-1 treated with 0.1µg/ml PMA for 48 hours, then with 0.045 µg/ml IL-4 added for 72 hours, whole cell lysate at 50 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 9 kDa

Observed band size: 10 kDa

false

Exposure time: 103s

• WB

Supplier Data

Western blot - Anti-CCL18 antibody [EPR24781-84] (BSA and Azide free) (AB300058)

This data was developed using ab300057, the same antibody clone in a different buffer formulation.

5% NFDM/TBST was used as blocking and diluting buffer and concentration.

THP-1 cells after PMA and IL-4 treatment secrete high levels of CCL18. (PMID : 25502147).

All lanes:

Western blot - Anti-CCL18 antibody [EPR24781-84] (<a href='/en-us/products/primary-antibodies/ccl18-antibody-epr24781-84-ab300057'>ab300057</a>) at 1/1000 dilution

Lane 1:

THP-1( Human monocytic leukemia monocyte) treated with 0.1µg/ml PMA for 48 hours whole cell lysate at 50 µg

Lane 2:

THP-1 treated with 0.1µg/ml PMA for 48 hours, then with 0.045 µg/ml IL-4 added for 72 hours, whole cell lysate at 50 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 10 kDa

Observed band size: 10 kDa

false

Exposure time: 103s

• Dot

Supplier Data

Dot Blot - Anti-CCL18 antibody [EPR24781-84] (BSA and Azide free) (AB300058)

This data was developed using ab300057, the same antibody clone in a different buffer formulation. Dot blot analysis of CCL18 using ab300057 at 1 : 1000 (0.491 µg/ml) followed by a Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1 : 100,000 dilution. Exposure time : 5.5 seconds Blocking and diluting buffer and concentration : 5% NFDM/TBST

• Dot

Supplier Data

Dot Blot - Anti-CCL18 antibody [EPR24781-84] (BSA and Azide free) (AB300058)

This data was developed using ab300057, the same antibody clone in a different buffer formulation. Dot blot analysis of CCL18 using ab300057 at 1 : 1000 (0.491 µg/ml) followed by a Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1 : 100,000 dilution. Exposure time : 3 minutes Blocking and diluting buffer and concentration : 5% NFDM/TBST

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CCL18 antibody [EPR24781-84] (BSA and Azide free) (AB300058)

This data was developed using ab300057, the same antibody clone in a different buffer formulation. Immunohistochemical analysis of paraffin-embedded Human stomach cancer tissue labelling CCL18 with ab300057 at 1/500 (0.982 µ/ml) followed by a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer). Positive staining on human stomach cancer. The section was incubated with ab300057 at 4°C overnight. Counterstained with Hematoxylin. Secondary antibody only control : Secondary antibody is a ready to use Goat Anti-Rabbit IgG H&L (HRP polymer). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins

• Dot

Supplier Data

Dot Blot - Anti-CCL18 antibody [EPR24781-84] (BSA and Azide free) (AB300058)

This data was developed using ab300057, the same antibody clone in a different buffer formulation.

Dot blot analysis of CCL18 using ab300057 at 1 : 1000 (0.491 μg/ml) followed by a Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1 : 100,000 dilution.

Exposuse time : 3 minutes

Blocking and diluting buffer and concentration : 5% NFDM/TBST

• Dot

Supplier Data

Dot Blot - Anti-CCL18 antibody [EPR24781-84] (BSA and Azide free) (AB300058)

This data was developed using ab300057, the same antibody clone in a different buffer formulation.

Dot blot analysis of CCL18 using ab300057 at 1 : 1000 (0.491 μg/ml) followed by a Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1 : 100,000 dilution.

Exposure time : 5.5 seconds

Blocking and diluting buffer and concentration : 5% NFDM/TBST