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AB9851

Anti-CCL21 antibody

4

(4 Reviews)

|

(8 Publications)

Rabbit Polyclonal CCL21 antibody. Suitable for ELISA, WB, sELISA, IHC-P and reacts with Human samples. Cited in 8 publications. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human CCL21.

View Alternative Names

SCYA21, UNQ784/PRO1600, CCL21, C-C motif chemokine 21, 6Ckine, Beta-chemokine exodus-2, Secondary lymphoid-tissue chemokine, Small-inducible cytokine A21, SLC

1 Images
Sandwich ELISA - Anti-CCL21 antibody (AB9851)
  • sELISA

Supplier Data

Sandwich ELISA - Anti-CCL21 antibody (AB9851)

Sandwich ELISA : To detect Human Exodus-2 (CCL21) by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.5-2.0 µg/mL of CCL21 Polyclonal Antibody (ab9851) is required. This antigen affinity purified antibody, in conjunction with PeproTech CCL21 Polyclonal Antibody, Biotin (ab83130) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human Exodus-2 (CCL21).

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

Unknown

Light chain type

unknown

Carrier free

No

Reacts with

Human

Applications

ELISA, WB, sELISA, IHC-P

applications

Immunogen

Recombinant Full Length Protein corresponding to Human CCL21.

O00585

Reactivity data

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Product details

Although some customers have been successful in IHC we no longer batch test in this application.

Properties and storage information

Form
Lyophilized
Reconstitution
Reconstitute with 200µl of sterile water. Please note that if you receive this product in liquid form it has already been reconstituted as described and no further reconstitution is necessary.
Purification technique
Affinity purification Immunogen
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CCL21 also known as Exodus 2 or 2H26 is a chemokine with significant roles in the immune system. It is a small protein with a molecular mass of approximately 12 kDa. CCL21 is expressed in high concentrations in secondary lymphoid organs such as the spleen lymph nodes and Peyer's patches. It serves as a chemoattractant particularly for lymphocytes and dendritic cells facilitating their migration towards lymphoid organs.
Biological function summary

CCL21 is essential for guiding immune cells to areas where they are needed for initiating immune responses. It forms a non-covalent complex with its receptor CCR7 a primary mechanism through which it exerts its biological effects. CCL21 coordinates the positioning of immune cells within the lymphoid organs ensuring a functional and effective immune response to pathogens or injuries. Its expression pattern aids in the organization of lymphoid tissue architecture and contributes to efficient trafficking of T-cells and dendritic cells.

Pathways

CCL21 plays an important role in the chemokine signaling pathway and the leukocyte transendothelial migration pathway. Within these pathways CCL21 works closely with its receptor CCR7 to guide immune cell navigation and localization. This collaboration is essential for directing cells to appropriate sites in the immune system aligning CCL21 with similar proteins like CCL19 another chemokine that also interacts with CCR7.

CCL21 is associated with several immune-related conditions including rheumatoid arthritis and chronic lymphocytic leukemia. In rheumatoid arthritis CCL21 contributes to the infiltration and retention of immune cells in the synovial tissue while in chronic lymphocytic leukemia it aids in the homing of malignant cells to lymphoid organs. CCL21’s interaction with CCR7 in these diseases highlights its importance not only in immune surveillance but also in the pathology of these disorders.

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Target data

Inhibits hemopoiesis and stimulates chemotaxis. Chemotactic in vitro for thymocytes and activated T-cells, but not for B-cells, macrophages, or neutrophils. Shows preferential activity towards naive T-cells. May play a role in mediating homing of lymphocytes to secondary lymphoid organs. Binds to atypical chemokine receptor ACKR4 and mediates the recruitment of beta-arrestin (ARRB1/2) to ACKR4.
See full target information CCL21

Publications (8)

Recent publications for all applications. Explore the full list and refine your search

Gastroenterology 159:665-681.e13 PubMed32330476

2020

CXCL10 and CCL21 Promote Migration of Pancreatic Cancer Cells Toward Sensory Neurons and Neural Remodeling in Tumors in Mice, Associated With Pain in Patients.

Applications

Unspecified application

Species

Unspecified reactive species

Michael Hirth,Jagadeesh Gandla,Christiane Höper,Matthias M Gaida,Nitin Agarwal,Manuela Simonetti,Aykut Demir,Yong Xie,Cleo Weiss,Christoph W Michalski,Thilo Hackert,Matthias P Ebert,Rohini Kuner

Cell death & disease 10:935 PubMed31819043

2019

BRD4 promotes tumor progression and NF-κB/CCL2-dependent tumor-associated macrophage recruitment in GIST.

Applications

Unspecified application

Species

Unspecified reactive species

Jianfeng Mu,Pengfei Sun,Zhiming Ma,Pengda Sun

The journal of histochemistry and cytochemistry : 67:299-308 PubMed30633620

2019

The Diagnostic Value of Chemokine/Chemokine Receptor Pairs in Hepatocellular Carcinoma and Colorectal Liver Metastasis.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaolei Jiao,Guiming Shu,Hui Liu,Qin Zhang,Zhe Ma,Chaoyi Ren,Hongsheng Guo,Jingxiang Shi,Junguo Liu,Chuanshan Zhang,Yijun Wang,Yingtang Gao

Bio-protocol 8: PubMed29780855

2018

Magnetic Resonance Imaging and Histopathological Visualization of Human Dural Lymphatic Vessels.

Applications

Unspecified application

Species

Unspecified reactive species

Seung-Kwon Ha,Govind Nair,Martina Absinta,Nicholas J Luciano,Daniel S Reich

eLife 6: PubMed28971799

2017

Human and nonhuman primate meninges harbor lymphatic vessels that can be visualized noninvasively by MRI.

Applications

Unspecified application

Species

Unspecified reactive species

Martina Absinta,Seung-Kwon Ha,Govind Nair,Pascal Sati,Nicholas J Luciano,Maryknoll Palisoc,Antoine Louveau,Kareem A Zaghloul,Stefania Pittaluga,Jonathan Kipnis,Daniel S Reich

Oncotarget 8:25650-25659 PubMed27783999

2016

Low CCL-21 expression associates with unfavorable postoperative prognosis of patients with metastatic renal cell carcinoma.

Applications

IHC

Species

Human

Ying Xiong,Li Liu,Jiajun Wang,Wei Xi,Yu Xia,Qi Bai,Yang Qu,Qilai Long,Jiejie Xu,Jianming Guo

Neurotherapeutics : the journal of the American Society for Experimental NeuroTherapeutics 10:520-38 PubMed23775067

2013

Cell cycle activation contributes to increased neuronal activity in the posterior thalamic nucleus and associated chronic hyperesthesia after rat spinal cord contusion.

Applications

IHC, WB

Species

Unspecified reactive species, Unspecified reactive species

Junfang Wu,Charles Raver,Chunshu Piao,Asaf Keller,Alan I Faden

The American journal of pathology 178:1662-75 PubMed21435450

2011

Association of T-zone reticular networks and conduits with ectopic lymphoid tissues in mice and humans.

Applications

Unspecified application

Species

Unspecified reactive species

Alexander Link,Debbie L Hardie,Stéphanie Favre,Mirjam R Britschgi,David H Adams,Michael Sixt,Jason G Cyster,Christopher D Buckley,Sanjiv A Luther
View all publications

Product promise

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