AB248260

Anti-CCL21 antibody [EPR6218] - BSA and Azide free

RabMAb
Recombinant
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(2 Publications )

Rabbit Recombinant Monoclonal CCL21 antibody. Carrier free. Suitable for WB and reacts with Human samples. Cited in 2 publications.

1 Images

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Western blot - Anti-CCL21 antibody [EPR6218] - BSA and Azide free (AB248260)

This data was developed using ab129014, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-CCL21 antibody [EPR6218] (<a href='/en-us/products/primary-antibodies/ccl21-antibody-epr6218-ab129014'>ab129014</a>) at 1/1000 dilution

Lane 1:

Human CCL21 recombinant protein at 0.01 µg

Lane 2:

Caco 2 cell lysate at 10 µg

Secondary

All lanes:

HRP labelled Goat anti-Rabbit IgG at 1/2000 dilution

Predicted band size: 15 kDa

false

Unconjugated

Anti-CCL21 antibody [EPR6218]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR6218

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Product details

ab248260 is the carrier-free version of ab129014.

Species reactivity
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species.
Please contact us for more information.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CCL21 also known as Exodus 2 or 2H26 is a chemokine with significant roles in the immune system. It is a small protein with a molecular mass of approximately 12 kDa. CCL21 is expressed in high concentrations in secondary lymphoid organs such as the spleen lymph nodes and Peyer's patches. It serves as a chemoattractant particularly for lymphocytes and dendritic cells facilitating their migration towards lymphoid organs.

Biological function summary

CCL21 is essential for guiding immune cells to areas where they are needed for initiating immune responses. It forms a non-covalent complex with its receptor CCR7 a primary mechanism through which it exerts its biological effects. CCL21 coordinates the positioning of immune cells within the lymphoid organs ensuring a functional and effective immune response to pathogens or injuries. Its expression pattern aids in the organization of lymphoid tissue architecture and contributes to efficient trafficking of T-cells and dendritic cells.

Pathways

CCL21 plays an important role in the chemokine signaling pathway and the leukocyte transendothelial migration pathway. Within these pathways CCL21 works closely with its receptor CCR7 to guide immune cell navigation and localization. This collaboration is essential for directing cells to appropriate sites in the immune system aligning CCL21 with similar proteins like CCL19 another chemokine that also interacts with CCR7.

CCL21 is associated with several immune-related conditions including rheumatoid arthritis and chronic lymphocytic leukemia. In rheumatoid arthritis CCL21 contributes to the infiltration and retention of immune cells in the synovial tissue while in chronic lymphocytic leukemia it aids in the homing of malignant cells to lymphoid organs. CCL21’s interaction with CCR7 in these diseases highlights its importance not only in immune surveillance but also in the pathology of these disorders.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Inhibits hemopoiesis and stimulates chemotaxis. Chemotactic in vitro for thymocytes and activated T-cells, but not for B-cells, macrophages, or neutrophils. Shows preferential activity towards naive T-cells. May play a role in mediating homing of lymphocytes to secondary lymphoid organs. Binds to atypical chemokine receptor ACKR4 and mediates the recruitment of beta-arrestin (ARRB1/2) to ACKR4.

See full target information CCL21

Alternative Names

SCYA21, UNQ784/PRO1600, CCL21, C-C motif chemokine 21, 6Ckine, Beta-chemokine exodus-2, Secondary lymphoid-tissue chemokine, Small-inducible cytokine A21, SLC

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Publications (2)

Recent publications for all applications. Explore the full listand refine your search

Cellular and molecular gastroenterology and hepatology 12:395-425 PubMed33676046

2021

Helicobacter pylori-Induced Rev-erbα Fosters Gastric Bacteria Colonization by Impairing Host Innate and Adaptive Defense.

Applications

Unspecified application

Species

Unspecified reactive species

Fang-Yuan Mao,Yi-Pin Lv,Chuan-Jie Hao,Yong-Sheng Teng,Yu-Gang Liu,Ping Cheng,Shi-Ming Yang,Weisan Chen,Tao Liu,Quan-Ming Zou,Rui Xie,Jing-Yu Xu,Yuan Zhuang

European journal of human genetics : EJHG 26:1752-1758 PubMed30089828

2018

Biallelic loss of function variants in COASY cause prenatal onset pontocerebellar hypoplasia, microcephaly, and arthrogryposis.

Applications

Unspecified application

Species

Unspecified reactive species

Tessa van Dijk,Sacha Ferdinandusse,Jos P N Ruiter,Mariëlle Alders,Inge B Mathijssen,Jillian S Parboosingh,A Micheil Innes,Hanne Meijers-Heijboer,Bwee Tien Poll-The,Francois P Bernier,Ronald J A Wanders,Ryan E Lamont,Frank Baas

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-CCL21 antibody [EPR6218] - BSA and Azide free

Western blot - Anti-CCL21 antibody [EPR6218] - BSA and Azide free (AB248260)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Complementary Products

Assay Kits

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Alternative Names

Publications (2)

Product promise