Rabbit Recombinant Monoclonal Ccl21a antibody. Carrier free. Suitable for sELISA and reacts with Mouse samples.
Images
![Sandwich ELISA - Anti-Ccl21a antibody [EPR18001-134] - BSA and Azide free (Detector) (AB242784), expandable thumbnail](https://content.abcam.com/products/images/ccl21a-antibody-epr18001-134-bsa-and-azide-free-detector-ab242784--sandwich-elisa-img138797.jpg/_jcr_content/renditions/webp-475.webp "Sandwich ELISA - Anti-Ccl21a antibody [EPR18001-134] - BSA and Azide free (Detector) (AB242784)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Constituents: 100% PBS
- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| sELISA | |
|---|---|
| Mouse | Expected |
sELISA
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
Target data
Function
Inhibits hemopoiesis and stimulates chemotaxis. Chemotactic in vitro for thymocytes and activated T-cells, but not for B-cells, macrophages, or neutrophils. Potent mesangial cell chemoattractant. Shows preferential activity towards naive T-cells. May play a role in mediating homing of lymphocytes to secondary lymphoid organs.
Alternative names
Scya21, Scya21a, Ccl21a, C-C motif chemokine 21a, 6Ckine, Beta-chemokine exodus-2, Small-inducible cytokine A21a, Thymus-derived chemotactic agent 4, TCA4
Recommended products
Rabbit Recombinant Monoclonal Ccl21a antibody. Carrier free. Suitable for sELISA and reacts with Mouse samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Constituents: 100% PBS
- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR18001-134
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
Notes
ab242784 is a BSA and Azide Free antibody supplied in an unconjugated format and it is suitable for sandwich ELISAs to quantify Mouse CCL21a. The recommended pair for sandwich ELISA is:
Capture: Anti-Ccl21a antibody [EP18001-19] - BSA and Azide free (Capture) ab242536, Mouse CCL21a Capture Antibody (unconjugated)
Detector: ab242784, Mouse CCL21a Detector Antibody (unconjugated)
The reference range value is 3.9 - 250 pg/ml.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
The recommended antibody orientation is based on internal optimization for ELISA-based assays. Antibody orientation is assay dependent and needs to be optimized for each assay type. Please note that the range provided for this antibody is only an estimation based on the performance of the product using the recommended antibody pair. Performance of the antibody pair will depend on the specific characteristics of your assay. We guarantee the product works in sandwich ELISA, but we do not guarantee the sensitivity or dynamic range of the antibody in your assay.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
C-C motif chemokine ligand 21a also known as Ccl21a or Exodus-2 is a chemokine with a molecular mass of approximately 16 kDa. It functions mechanically by acting as a ligand for the CCR7 receptor which plays a significant role in immune responses. Ccl21a is mainly expressed in lymphoid tissues such as the lymph nodes and the spleen where it guides naive T-cells and dendritic cells to these regions by creating a chemotactic gradient.
- Biological function summary
The actions of Ccl21a are important for the immune system's homeostasis and surveillance. It helps maintain the organized structure of lymphoid tissues by ensuring the correct positioning of lymphocytes. Ccl21a operates as part of the network that forms the microenvironment necessary for immune cell interactions and antigen presentation. There are no notable reports of it forming part of a larger protein complex but its receptor CCR7 is an essential player in its function.
- Pathways
Ccl21a is involved in the chemokine signaling pathway and the regulation of immune cell trafficking. It interacts heavily within the pathway that involves the engagement of CCR7 a receptor that Ccl21a primarily activates alongside its counterpart ligand CCL19. Through these interactions Ccl21a influences processes related to lymphocyte migration and adaptive immune responses.
- Associated diseases and disorders
Disruptions in Ccl21a expression or function associate with conditions like autoimmune diseases and chronic inflammation. Abnormalities in its signaling can result in diseases such as rheumatoid arthritis due to inappropriate recruitment and positioning of immune cells. Ccl21a's interaction with proteins like CCR7 links it to these disorders as aberrant CCR7 engagement influences immune cell dynamics in diseased states.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
1 product image
![Sandwich ELISA - Anti-Ccl21a antibody [EPR18001-134] - BSA and Azide free (Detector) (ab242784), expandable thumbnail](https://content.abcam.com/products/images/ccl21a-antibody-epr18001-134-bsa-and-azide-free-detector-ab242784--sandwich-elisa-img138797.jpg "Sandwich ELISA - Anti-Ccl21a antibody [EPR18001-134] - BSA and Azide free (Detector) (ab242784)")
Sandwich ELISA - Anti-Ccl21a antibody [EPR18001-134] - BSA and Azide free (Detector) (ab242784)
Representative standard curve from corresponding SimpleStep ELISA® Kit (Mouse CCL21a ELISA Kit ab208985).
Downloads
Product protocols
- Visit the general protocols
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