AB228323

Anti-CCL27 antibody [EPR17284-217] - BSA and Azide free

RabMAb
Recombinant
What is this?

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(1 Publication)

Rabbit Recombinant Monoclonal CCL27 antibody. Carrier free. Suitable for IP, WB and reacts with Mouse samples. Cited in 1 publication.

View Alternative Names

Ilc, Scya27, Ccl27, C-C motif chemokine 27, CC chemokine ILC, Cutaneous T-cell-attracting chemokine, ESkine, IL-11 R-alpha-locus chemokine, Skinkine, Small-inducible cytokine A27, CTACK, ALP, mILC

2 Images

Supplier Data

Immunoprecipitation - Anti-CCL27 antibody [EPR17284-217] - BSA and Azide free (AB228323)

CCL27 was immunoprecipitated from 0.35 mg of mouse skin lysate with ab181323 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab181323 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/10000 dilution.

Lane 1 : Mouse skin lysate 10 μg (Input).

Lane 2 : ab181323 IP in mouse skin lysate (+).

Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab181323 in mouse skin lysate (-).

Blocking and dilution buffer and concentration : 5% NFDM/TBST.

Exposure time : 3 minutes.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab181323).

All lanes:

Immunoprecipitation - Anti-CCL27 antibody [EPR17284-217] (<a href='/en-us/products/primary-antibodies/ccl27-antibody-epr17284-217-ab181323'>ab181323</a>)

Predicted band size: 13 kDa

false

Supplier Data

Western blot - Anti-CCL27 antibody [EPR17284-217] - BSA and Azide free (AB228323)

Blocking/Dilution buffer : 5% NFDM/TBST.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab181323).

All lanes:

Western blot - Anti-CCL27 antibody [EPR17284-217] (<a href='/en-us/products/primary-antibodies/ccl27-antibody-epr17284-217-ab181323'>ab181323</a>) at 1/1000 dilution

All lanes:

Mouse skin lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Predicted band size: 13 kDa

Observed band size: 13 kDa

true

Exposure time: 5s

Carrier free

Anti-CCL27 antibody [EPR17284-217] - BSA and Azide free (Capture)
Unconjugated

Anti-CCL27 antibody [EPR17284-217]

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR17284-217

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse

Applications

IP, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" } } }

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Product details

ab228323 is the carrier-free version of ab181323.

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar^® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar^® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

+4°C

Storage information

Do Not Freeze

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CCL27 also known as CTACK (cutaneous T cell-attracting chemokine) is a chemokine that plays a critical role in skin immune surveillance. It has a mass of approximately 10 kDa. CCL27 is expressed in keratinocytes which are the predominant cells in the epidermis. It is also secreted by cells in inflamed skin. Researchers often use ELISA (enzyme-linked immunosorbent assay) techniques to study CCL27 due to its importance in skin immunology.

Biological function summary

CCL27 attracts T cells to the skin's surface facilitating immune responses. Although not part of a larger complex CCL27 interacts with its receptor CCR10 on T cells directing them to skin sites where they can perform immune surveillance and respond to pathogens. This chemokine-receptor interaction is essential for maintaining skin homeostasis and effective immune responses.

Pathways

The role of CCL27 gets significant attention in the CCR10-CTACK axis which is pivotal in the cutaneous immune system's functioning. Additionally the CCL27/CCR10 pathway has connections to the wider chemokine signaling network that regulates leukocyte trafficking. Through these associations CCL27 is connected to other chemokines and receptors including CCL28 which also shares CCR10 as a receptor highlighting the integrated nature of chemokine function in immune pathways.

CCL27 has been linked to various skin conditions such as psoriasis and atopic dermatitis. In psoriasis CCL27 recruits CCR10-expressing T cells to the skin aggravating the inflammatory response. Similarly in atopic dermatitis increased CCL27 levels may contribute to disease pathology by attracting immune cells that perpetuate inflammation. Understanding the interaction between CCL27 and these conditions may offer insight into potential therapeutic targets aimed at modulating chemokine signaling to alleviate symptoms.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Chemotactic factor that attracts skin-associated memory T-lymphocytes. May play a role in mediating homing of lymphocytes to cutaneous sites. May play a role in cell migration during embryogenesis. Nuclear forms may facilitate cellular migration by inducing cytoskeletal relaxation. Binds to CCR10.

See full target information Ccl27

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Frontiers in oncology 10:950 PubMed32612954

2020

Independent Prognostic Value of Intratumoral Heterogeneity and Immune Response Features by Automated Digital Immunohistochemistry Analysis in Early Hormone Receptor-Positive Breast Carcinoma.

Applications

Unspecified application

Species

Unspecified reactive species

Dovile Zilenaite,Allan Rasmusson,Renaldas Augulis,Justinas Besusparis,Aida Laurinaviciene,Benoit Plancoulaine,Valerijus Ostapenko,Arvydas Laurinavicius

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-CCL27 antibody [EPR17284-217] - BSA and Azide free

Immunoprecipitation - Anti-CCL27 antibody [EPR17284-217] - BSA and Azide free (AB228323)

Western blot - Anti-CCL27 antibody [EPR17284-217] - BSA and Azide free (AB228323)

Related conjugates and formulations (2)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Product promise