AB186421

Anti-CCL27 antibody [EPR8582(2)]

RabMAb
Recombinant
What is this?

Be the first to review this product! Submit a review

(7 Publications)

Rabbit Recombinant Monoclonal CCL27 antibody. Suitable for WB, ICC/IF, Flow Cyt (Intra) and reacts with Human samples. Cited in 7 publications.

View Alternative Names

ILC, SCYA27, CCL27, C-C motif chemokine 27, CC chemokine ILC, Cutaneous T-cell-attracting chemokine, ESkine, IL-11 R-alpha-locus chemokine, Skinkine, Small-inducible cytokine A27, CTACK

3 Images

Immunocytochemistry/ Immunofluorescence - Anti-CCL27 antibody [EPR8582(2)] (AB186421)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-CCL27 antibody [EPR8582(2)] (AB186421)

Immunofluorescent analysis of 4% paraformaldehyde-fixed A431 cells labeling CCL27 with ab186421 at 1/250 dilution followed by Goat anti rabbit IgG (Alexa Fluor® 555) secondary antibody at 1/200 dilution. Counter stained with Dapi.

Flow Cytometry (Intracellular) - Anti-CCL27 antibody [EPR8582(2)] (AB186421)

Flow Cyt (Intra)

Supplier Data

Flow Cytometry (Intracellular) - Anti-CCL27 antibody [EPR8582(2)] (AB186421)

Intracellular flow cytometric analysis of 2% paraformaldehyde-fixed HACAT cells labeling CCL27 with ab186421 at 1/320 dilution (red) compared to a Rabbit monoclonal IgG isotype control (green), followed by Goat anti rabbit IgG (FITC) secondary antibody at 1/150 dilution.

Supplier Data

Western blot - Anti-CCL27 antibody [EPR8582(2)] (AB186421)

All lanes:

Western blot - Anti-CCL27 antibody [EPR8582(2)] (ab186421) at 1/5000 dilution

All lanes:

Human CCL27 recombinant protein at 0.01 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 13 kDa

false

Carrier free

Anti-CCL27 antibody [EPR8582(2)] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR8582(2)

Isotype

IgG

Carrier free

Reacts with

Human

Applications

ICC/IF, WB, Flow Cyt (Intra)

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "FlowCytIntra" : {"fullname" : "Flow Cytometry (Intracellular)", "shortname":"Flow Cyt (Intra)"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/10000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/250", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "testedAndGuaranteed", "FlowCytIntra-species-dilution-info": "1/320", "FlowCytIntra-species-notes": "<a href='/en-us/products/primary-antibodies/rabbit-igg-monoclonal-epr25a-isotype-control-ab172730'>ab172730</a> - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody." }, "Mouse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" }, "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "predicted", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "", "FlowCytIntra-species-checked": "predicted", "FlowCytIntra-species-dilution-info": "", "FlowCytIntra-species-notes": "" } } }

style

left-column

Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CCL27 also known as CTACK (cutaneous T cell-attracting chemokine) is a chemokine that plays a critical role in skin immune surveillance. It has a mass of approximately 10 kDa. CCL27 is expressed in keratinocytes which are the predominant cells in the epidermis. It is also secreted by cells in inflamed skin. Researchers often use ELISA (enzyme-linked immunosorbent assay) techniques to study CCL27 due to its importance in skin immunology.

Biological function summary

CCL27 attracts T cells to the skin's surface facilitating immune responses. Although not part of a larger complex CCL27 interacts with its receptor CCR10 on T cells directing them to skin sites where they can perform immune surveillance and respond to pathogens. This chemokine-receptor interaction is essential for maintaining skin homeostasis and effective immune responses.

Pathways

The role of CCL27 gets significant attention in the CCR10-CTACK axis which is pivotal in the cutaneous immune system's functioning. Additionally the CCL27/CCR10 pathway has connections to the wider chemokine signaling network that regulates leukocyte trafficking. Through these associations CCL27 is connected to other chemokines and receptors including CCL28 which also shares CCR10 as a receptor highlighting the integrated nature of chemokine function in immune pathways.

CCL27 has been linked to various skin conditions such as psoriasis and atopic dermatitis. In psoriasis CCL27 recruits CCR10-expressing T cells to the skin aggravating the inflammatory response. Similarly in atopic dermatitis increased CCL27 levels may contribute to disease pathology by attracting immune cells that perpetuate inflammation. Understanding the interaction between CCL27 and these conditions may offer insight into potential therapeutic targets aimed at modulating chemokine signaling to alleviate symptoms.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

Chemotactic factor that attracts skin-associated memory T-lymphocytes. May play a role in mediating homing of lymphocytes to cutaneous sites. Binds to CCR10.

See full target information CCL27

style

left-column

Publications (7)

Recent publications for all applications. Explore the full list and refine your search

Scientific reports 15:19950 PubMed40481048

2025

CircRNA_1809 promotes the osteogenic differentiation of bone marrow mesenchymal stem cells through miR-370-3p.

Applications

Unspecified application

Species

Unspecified reactive species

Lei Zhang,Yidan Xu,Ningning Liu,Mengjie Xu,Refeina Tuerxun,Mireyeti Shahatibieke,Yuan Yao

Journal of inflammation research 17:5177-5195 PubMed39104909

2024

Screening of Diagnostic Biomarkers and Immune Infiltration Characteristics Linking Rheumatoid Arthritis and Rosacea Based on Bioinformatics Analysis.

Applications

Unspecified application

Species

Unspecified reactive species

Yun Wang,Jun Chen,Zheng-Yu Shen,Jie Zhang,Yu-Jie Zhu,Xu-Qiong Xia

Journal of biochemical and molecular toxicology 36:e23213 PubMed36111657

2022

Bradykinin/bradykinin 1 receptor promotes brain microvascular endothelial cell permeability and proinflammatory cytokine release by downregulating Wnt3a.

Applications

Unspecified application

Species

Unspecified reactive species

Linqiang Huang,Mengting Liu,Wenqiang Jiang,Hongguang Ding,Yongli Han,Miaoyun Wen,Ya Li,Xiaoyu Liu,Hongke Zeng

Frontiers in pharmacology 12:756671 PubMed34899311

2021

Bilateral Paraventricular Nucleus Upregulation of Extracellular Superoxide Dismutase Decreases Blood Pressure by Regulation of the NLRP3 and Neurotransmitters in Salt-Induced Hypertensive Rats.

Applications

Unspecified application

Species

Unspecified reactive species

Qing Su,Xiao-Jing Yu,Xiao-Min Wang,Hong-Bao Li,Ying Li,Juan Bai,Jie Qi,Nianping Zhang,Kai-Li Liu,Yan Zhang,Guo-Qing Zhu,Yu-Ming Kang

Journal of translational medicine 19:338 PubMed34372858

2021

CYB561D2 up-regulation activates STAT3 to induce immunosuppression and aggression in gliomas.

Applications

Unspecified application

Species

Unspecified reactive species

Bangbao Tao,Juanhong Shi,Shuai Shuai,Haiyan Zhou,Hongxia Zhang,Bin Li,Xiaoqiang Wang,Guohui Li,Hua He,Jun Zhong

Respiratory research 22:52 PubMed33579290

2021

Qi-Long-Tian formula extract alleviates symptoms of acute high-altitude diseases via suppressing the inflammation responses in rat.

Applications

Unspecified application

Species

Unspecified reactive species

Xing Fu,Chunyan Yang,Bing Chen,Kexing Zeng,Siyuan Chen,Yi Fu

The FEBS journal 285:2278-2291 PubMed29676849

2018

HOXC10 promotes proliferation and invasion and induces immunosuppressive gene expression in glioma.

Applications

Unspecified application

Species

Unspecified reactive species

Shu Li,Wenhao Zhang,Chao Wu,Hongliang Gao,Jun Yu,Xiaoqiang Wang,Bin Li,Zhong Jun,Wenchaun Zhang,Ping Zhou,Juanhong Shi,Lifeng Wang,Yunxing Gao,Shiting Li,Bangbao Tao

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-CCL27 antibody [EPR8582(2)]

Immunocytochemistry/ Immunofluorescence - Anti-CCL27 antibody [EPR8582(2)] (AB186421)

Flow Cytometry (Intracellular) - Anti-CCL27 antibody [EPR8582(2)] (AB186421)

Western blot - Anti-CCL27 antibody [EPR8582(2)] (AB186421)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (7)

Alternative Products

Primary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Complementary Products

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Product promise