Rabbit Recombinant Monoclonal CCL27 antibody. Carrier free. Suitable for ICC/IF, WB, Flow Cyt (Intra) and reacts with Human samples.
Images
![Immunocytochemistry/ Immunofluorescence - Anti-CCL27 antibody [EPR8582(2)] - BSA and Azide free (AB250878), expandable thumbnail](https://content.abcam.com/products/images/ccl27-antibody-epr85822-bsa-and-azide-free-ab250878--immunocytochemistry-immunofluorescence-img960.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-CCL27 antibody [EPR8582(2)] - BSA and Azide free (AB250878)")
![Western blot - Anti-CCL27 antibody [EPR8582(2)] - BSA and Azide free (AB250878), expandable thumbnail](https://content.abcam.com/products/images/ccl27-antibody-epr85822-bsa-and-azide-free-ab250878--western-blot-img81839.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-CCL27 antibody [EPR8582(2)] - BSA and Azide free (AB250878)")
![Flow Cytometry (Intracellular) - Anti-CCL27 antibody [EPR8582(2)] - BSA and Azide free (AB250878), expandable thumbnail](https://content.abcam.com/products/images/ccl27-antibody-epr85822-bsa-and-azide-free-ab250878--flow-cytometry-intracellular-img68635.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-CCL27 antibody [EPR8582(2)] - BSA and Azide free (AB250878)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| ICC/IF | WB | Flow Cyt (Intra) | |
|---|---|---|---|
| Human | Tested | Tested | Tested |
| Mouse | Predicted | Predicted | Predicted |
| Rat | Predicted | Predicted | Predicted |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Target data
Function
Chemotactic factor that attracts skin-associated memory T-lymphocytes. May play a role in mediating homing of lymphocytes to cutaneous sites. Binds to CCR10.
Alternative names
ILC, SCYA27, CCL27, C-C motif chemokine 27, CC chemokine ILC, Cutaneous T-cell-attracting chemokine, ESkine, IL-11 R-alpha-locus chemokine, Skinkine, Small-inducible cytokine A27, CTACK
Recommended products
Rabbit Recombinant Monoclonal CCL27 antibody. Carrier free. Suitable for ICC/IF, WB, Flow Cyt (Intra) and reacts with Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR8582(2)
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab250878 is the carrier-free version of Anti-CCL27 antibody [EPR8582(2)] ab186421.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
CCL27 also known as CTACK (cutaneous T cell-attracting chemokine) is a chemokine that plays a critical role in skin immune surveillance. It has a mass of approximately 10 kDa. CCL27 is expressed in keratinocytes which are the predominant cells in the epidermis. It is also secreted by cells in inflamed skin. Researchers often use ELISA (enzyme-linked immunosorbent assay) techniques to study CCL27 due to its importance in skin immunology.
- Biological function summary
CCL27 attracts T cells to the skin's surface facilitating immune responses. Although not part of a larger complex CCL27 interacts with its receptor CCR10 on T cells directing them to skin sites where they can perform immune surveillance and respond to pathogens. This chemokine-receptor interaction is essential for maintaining skin homeostasis and effective immune responses.
- Pathways
The role of CCL27 gets significant attention in the CCR10-CTACK axis which is pivotal in the cutaneous immune system's functioning. Additionally the CCL27/CCR10 pathway has connections to the wider chemokine signaling network that regulates leukocyte trafficking. Through these associations CCL27 is connected to other chemokines and receptors including CCL28 which also shares CCR10 as a receptor highlighting the integrated nature of chemokine function in immune pathways.
- Associated diseases and disorders
CCL27 has been linked to various skin conditions such as psoriasis and atopic dermatitis. In psoriasis CCL27 recruits CCR10-expressing T cells to the skin aggravating the inflammatory response. Similarly in atopic dermatitis increased CCL27 levels may contribute to disease pathology by attracting immune cells that perpetuate inflammation. Understanding the interaction between CCL27 and these conditions may offer insight into potential therapeutic targets aimed at modulating chemokine signaling to alleviate symptoms.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
![Immunocytochemistry/ Immunofluorescence - Anti-CCL27 antibody [EPR8582(2)] - BSA and Azide free (ab250878), expandable thumbnail](https://content.abcam.com/products/images/ccl27-antibody-epr85822-bsa-and-azide-free-ab250878--immunocytochemistry-immunofluorescence-img960.jpg "Immunocytochemistry/ Immunofluorescence - Anti-CCL27 antibody [EPR8582(2)] - BSA and Azide free (ab250878)")
Immunocytochemistry/ Immunofluorescence - Anti-CCL27 antibody [EPR8582(2)] - BSA and Azide free (ab250878)
This data was developed using Anti-CCL27 antibody [EPR8582(2)] ab186421, the same antibody clone in a different buffer formulation.Immunofluorescent analysis of 4% paraformaldehyde-fixed A431 cells labeling CCL27 with Anti-CCL27 antibody [EPR8582(2)] ab186421 at 1/250 dilution followed by Goat anti rabbit IgG (Alexa Fluor® 555) secondary antibody at 1/200 dilution. Counter stained with Dapi.
![Western blot - Anti-CCL27 antibody [EPR8582(2)] - BSA and Azide free (ab250878), expandable thumbnail](https://content.abcam.com/products/images/ccl27-antibody-epr85822-bsa-and-azide-free-ab250878--western-blot-img81839.jpg "Western blot - Anti-CCL27 antibody [EPR8582(2)] - BSA and Azide free (ab250878)")
Western blot - Anti-CCL27 antibody [EPR8582(2)] - BSA and Azide free (ab250878)
This data was developed using Anti-CCL27 antibody [EPR8582(2)] ab186421, the same antibody clone in a different buffer formulation.
All lanes: Western blot - Anti-CCL27 antibody [EPR8582(2)] (Anti-CCL27 antibody [EPR8582(2)] ab186421) at 1/5000 dilution
All lanes: Human CCL27 recombinant protein at 0.01 µg
SecondaryAll lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 13 kDa
![Flow Cytometry (Intracellular) - Anti-CCL27 antibody [EPR8582(2)] - BSA and Azide free (ab250878), expandable thumbnail](https://content.abcam.com/products/images/ccl27-antibody-epr85822-bsa-and-azide-free-ab250878--flow-cytometry-intracellular-img68635.jpg "Flow Cytometry (Intracellular) - Anti-CCL27 antibody [EPR8582(2)] - BSA and Azide free (ab250878)")
Flow Cytometry (Intracellular) - Anti-CCL27 antibody [EPR8582(2)] - BSA and Azide free (ab250878)
This data was developed using Anti-CCL27 antibody [EPR8582(2)] ab186421, the same antibody clone in a different buffer formulation.
Intracellular flow cytometric analysis of 2% paraformaldehyde-fixed HACAT cells labeling CCL27 with Anti-CCL27 antibody [EPR8582(2)] ab186421 at 1/320 dilution (red) compared to a Rabbit monoclonal IgG isotype control (green), followed by Goat anti rabbit IgG (FITC) secondary antibody at 1/150 dilution.
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