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AB251188

Anti-CCL27 antibody [EPR8583(2)] - BSA and Azide free

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(1 Publication)

Rabbit Recombinant Monoclonal CCL27 antibody. Carrier free. Suitable for ICC/IF, WB and reacts with Human samples. Cited in 1 publication.

View Alternative Names

ILC, SCYA27, CCL27, C-C motif chemokine 27, CC chemokine ILC, Cutaneous T-cell-attracting chemokine, ESkine, IL-11 R-alpha-locus chemokine, Skinkine, Small-inducible cytokine A27, CTACK

2 Images
Immunocytochemistry/ Immunofluorescence - Anti-CCL27 antibody [EPR8583(2)] - BSA and Azide free (AB251188)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-CCL27 antibody [EPR8583(2)] - BSA and Azide free (AB251188)

This data was developed using ab194354, the same antibody clone in a different buffer formulation.

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized A431 cells labeling CCL27 with ab194354 at 1/50 dilution, followed by Goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) secondary antibody at 1/400 dilution. The nuclear counter stain is DAPI (blue).

Western blot - Anti-CCL27 antibody [EPR8583(2)] - BSA and Azide free (AB251188)
  • WB

Supplier Data

Western blot - Anti-CCL27 antibody [EPR8583(2)] - BSA and Azide free (AB251188)

This data was developed using ab194354, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

All lanes:

Western blot - Anti-CCL27 antibody [EPR8583(2)] (<a href='/en-us/products/primary-antibodies/ccl27-antibody-epr85832-ab194354'>ab194354</a>) at 1/20000 dilution

All lanes:

Human CCL27 Recombinant protein at 0.01 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Predicted band size: 13 kDa

Observed band size: 13 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR8583(2)

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

ICC/IF, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" } } }
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Product details

ab251188 is the carrier-free version of ab194354.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CCL27 also known as CTACK (cutaneous T cell-attracting chemokine) is a chemokine that plays a critical role in skin immune surveillance. It has a mass of approximately 10 kDa. CCL27 is expressed in keratinocytes which are the predominant cells in the epidermis. It is also secreted by cells in inflamed skin. Researchers often use ELISA (enzyme-linked immunosorbent assay) techniques to study CCL27 due to its importance in skin immunology.
Biological function summary

CCL27 attracts T cells to the skin's surface facilitating immune responses. Although not part of a larger complex CCL27 interacts with its receptor CCR10 on T cells directing them to skin sites where they can perform immune surveillance and respond to pathogens. This chemokine-receptor interaction is essential for maintaining skin homeostasis and effective immune responses.

Pathways

The role of CCL27 gets significant attention in the CCR10-CTACK axis which is pivotal in the cutaneous immune system's functioning. Additionally the CCL27/CCR10 pathway has connections to the wider chemokine signaling network that regulates leukocyte trafficking. Through these associations CCL27 is connected to other chemokines and receptors including CCL28 which also shares CCR10 as a receptor highlighting the integrated nature of chemokine function in immune pathways.

CCL27 has been linked to various skin conditions such as psoriasis and atopic dermatitis. In psoriasis CCL27 recruits CCR10-expressing T cells to the skin aggravating the inflammatory response. Similarly in atopic dermatitis increased CCL27 levels may contribute to disease pathology by attracting immune cells that perpetuate inflammation. Understanding the interaction between CCL27 and these conditions may offer insight into potential therapeutic targets aimed at modulating chemokine signaling to alleviate symptoms.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Chemotactic factor that attracts skin-associated memory T-lymphocytes. May play a role in mediating homing of lymphocytes to cutaneous sites. Binds to CCR10.
See full target information CCL27
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

International journal of medical sciences 20:1079-1090 PubMed37484809

2023

NAT10-mediated RNA acetylation enhances HNRNPUL1 mRNA stability to contribute cervical cancer progression.

Applications

Unspecified application

Species

Unspecified reactive species

Yingfei Long,Yifei Ren,Qinglv Wei,Youchaou Mobet,Yujiao Liu,Hongyan Zhao,Tao Liu,Lei Cheng,Ping Yi
View all publications
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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