JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB251136

Anti-CCL28/MEC antibody [EPR12933] - BSA and Azide free

Be the first to review this product! Submit a review

|

(0 Publication)

Rabbit Recombinant Monoclonal CCL28/MEC antibody. Carrier free. Suitable for WB and reacts with Recombinant full length protein, Human samples.

View Alternative Names

SCYA28, CCL28, C-C motif chemokine 28, Mucosae-associated epithelial chemokine, Protein CCK1, Small-inducible cytokine A28, MEC

2 Images
Western blot - Anti-CCL28/MEC antibody [EPR12933] - BSA and Azide free (AB251136)
  • WB

Supplier Data

Western blot - Anti-CCL28/MEC antibody [EPR12933] - BSA and Azide free (AB251136)

This data was developed using ab192600, the same antibody clone in a different buffer formulation.

All lanes:

Western blot - Anti-CCL28/MEC antibody [EPR12933] (<a href='/en-us/products/primary-antibodies/ccl28-mec-antibody-epr12933-ab192600'>ab192600</a>) at 1/50000 dilution

All lanes:

CCL28/MEC recombinant protein at 10 µg

Secondary

All lanes:

goat anti-rabbit IgG, (H+L), peroxidase conjugated at 1/1000 dilution

Predicted band size: 14 kDa

Observed band size: 14 kDa

true

Western blot - Anti-CCL28/MEC antibody [EPR12933] - BSA and Azide free (AB251136)
  • WB

Lab

Western blot - Anti-CCL28/MEC antibody [EPR12933] - BSA and Azide free (AB251136)

This data was developed using ab192600, the same antibody clone in a different buffer formulation.

Blocking and dilution buffer : 5% NFDM/TBST.

This target is mainly expressed in some exocrine glands such as mammary gland and salivary gland, and is secreted into milk or saliva (PMID : 20688401; 12538707).

All lanes:

Western blot - Anti-CCL28/MEC antibody [EPR12933] (<a href='/en-us/products/primary-antibodies/ccl28-mec-antibody-epr12933-ab192600'>ab192600</a>) at 1/1000 dilution

All lanes:

Human milk at 10 µg

Secondary

All lanes:

Western blot - VeriBlot for IP Detection Reagent (HRP) (<a href='/en-us/products/reagents/veriblot-for-ip-detection-reagent-hrp-ab131366'>ab131366</a>) at 1/10000 dilution

Predicted band size: 14 kDa

Observed band size: 14 kDa

false

Exposure time: 3min

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR12933

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" }, "Recombinant full length protein": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>" } } }
style
left-column

You may be interested in:

AB99988

Human CCL28 ELISA Kit

0

0 Reviews

View product

We recommend this product because it’s often used in the same experiment or related research.

We advise that you always check the datasheet to ensure it fits your experiments, or contact ourtechnical teamfor help.

style
left-column

Product details

ab251136 is the carrier-free version of ab192600.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

style
left-column

Properties and storage information

Form
Liquid
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CCL28 also known as MEC (Mucosae-associated epithelial chemokine) is a chemokine weighing around 12 kDa. It plays a mechanical role in mediating cell chemotaxis promoting the migration of cells towards it. This chemokine predominantly expresses in mucosal tissues including the salivary glands intestines and respiratory tract epithelia. CCL28 interacts specifically with chemokine receptors CCR10 and CCR3 guiding immune cells to these mucosal surfaces where they perform important immune functions.
Biological function summary

CCL28 plays an important role in mucosal immunity and is involved in the defense against pathogens in these environments. The chemokine attracts lymphocytes and neutrophils aiding in their positioning at mucosal sites. This activity assists in maintaining a balanced immune response preventing excessive inflammation while ensuring adequate pathogen clearance. CCL28 is not part of a larger protein complex but performs critical functions independently coordinating with other immune mediators for effective response.

Pathways

The function of CCL28 aligns with its involvement in immune signaling and mucosal defense pathways. The chemokine integrates into the immune response network working closely with other chemokines and cytokines like CCL27 and IL-8. It engages in the chemokine signaling pathway which regulates leukocyte migration and positioning. The interplay between CCL28 and these immune mediators ensures proper protection of mucosal surfaces from potential pathogens.

CCL28 holds significant relevance in inflammatory bowel disease (IBD) and allergic rhinitis. In IBD the chemokine contributes to the infiltration of immune cells into the inflamed gut mucosa where its interaction with other cytokines such as TNF-α exacerbates tissue inflammation. In allergic rhinitis CCL28 participates in recruiting eosinophils to the nasal mucosa often correlating with heightened inflammatory responses in these patients and cooperating with proteins like IL-5 to sustain the allergic reaction.
style
left-column
style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Chemotactic activity for resting CD4, CD8 T-cells and eosinophils. Binds to CCR3 and CCR10 and induces calcium mobilization in a dose-dependent manner.
See full target information CCL28
style
left-column
style
left-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions
style
left-column
style
right-column
style
bg-slate-100

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com