AB106548

Anti-CCL4/MIP-1 beta antibody

Name: Anti-CCL4/MIP-1 beta antibody (ab106548) | Abcam
Brand: Abcam Limited
SKU: AB106548
Price: 535 USD
Availability: InStock

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(1 Publication)

Rabbit Polyclonal CCL4/MIP-1 beta antibody. Suitable for WB and reacts with Recombinant full length protein - Pig samples. Cited in 1 publication. Immunogen corresponding to Recombinant Full Length Protein corresponding to Pig CCL4 aa 1 to C-terminus.

View Alternative Names

MIP1B, SCY4A, CCL4, C-C motif chemokine 4, Macrophage inflammatory protein 1-beta, Small-inducible cytokine A4, MIP-1-beta

1 Images

Unknown

Western blot - Anti-CCL4/MIP-1 beta antibody (AB106548)

All lanes:

Western blot - Anti-CCL4/MIP-1 beta antibody (ab106548) at 4 µg/mL

All lanes:

Recombinant Pig CCL4/MIP-1 beta.

Secondary

All lanes:

IRDye800™ Conjugated Goat Anti-Rabbit IgG at 1/20000 dilution

Predicted band size: 10 kDa

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Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Reacts with

Pig

Applications

applications

Immunogen

Recombinant Full Length Protein corresponding to Pig CCL4 aa 1 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q711P4

Specificity

A BLAST analysis was used to suggest cross-reactivity with CCL4 from swine sources based on 100% homology with the immunizing sequence. Partial reactivity is expected against horse and panda CCL4 based on 90% homology. Cross-reactivity with CCL4 from other sources has not been determined. The swine CCL4 sequence is also 86% homologous to swine CCL3L1.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Rat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cat": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Chimpanzee": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Cow": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Dog": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Horse": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Macaque monkey": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Opossum": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Pig": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rabbit": { "WB-species-checked": "predicted", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Recombinant full length protein - Pig": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500", "WB-species-notes": "<p></p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Purification notes

This product was Protein-A purified from monospecific antiserum by chromatography.

Storage buffer

pH: 7.2 Preservative: 0.01% Sodium azide Constituents: 0.88% Sodium chloride, 0.424% Potassium phosphate solution

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CCL4 also known as MIP-1 beta or MIP-1b has a mechanical role as a chemokine involved in immune response regulation. The protein has a mass of about 7.8 kDa. It expresses in various tissues including leukocytes where it mainly functions as a chemoattractant. MIP-1 beta helps recruit immune cells like natural killer cells and monocytes to sites of inflammation or injury. Researchers frequently measure its levels using assays like MIP-1 beta ELISA or CCL4 ELISA.

Biological function summary

CCL4 is critical in immune system modulation. It coordinates leukocyte activation and trafficking contributing to inflammation and immune surveillance. It does not function in isolation but rather interacts with other chemokines and receptors. CCL4 alongside its sibling chemokines forms a dynamic network that ensures a balanced immune response. It connects to receptors such as CCR5 playing substantial roles in its activities.

Pathways

CCL4 participates in essential signaling cascades that mediate immune responses. It is notably part of the chemokine signaling pathway which is instrumental in directing cell movement. CCL4 also engages with proteins like CCR5 in the inflammatory and immune response pathways. This interaction enhances communication between cells required for effective pathogen defense.

CCL4 plays roles in immune-related conditions. It is strongly linked to inflammatory diseases such as rheumatoid arthritis and HIV infection. In rheumatoid arthritis CCL4 levels are often elevated correlating with inflammation severity. During HIV infection CCL4 interacts with proteins like CCR5 a co-receptor the virus uses to enter host cells. This involvement makes MIP-1 beta a target for studies seeking therapeutic interventions in these diseases.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Monokine with inflammatory and chemokinetic properties.

See full target information CCL4

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 9:389 PubMed29374163

2018

Mitochondrial levels determine variability in cell death by modulating apoptotic gene expression.

Applications

Unspecified application

Species

Unspecified reactive species

Silvia Márquez-Jurado,Juan Díaz-Colunga,Ricardo Pires das Neves,Antonio Martinez-Lorente,Fernando Almazán,Raúl Guantes,Francisco J Iborra

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-CCL4/MIP-1 beta antibody

Western blot - Anti-CCL4/MIP-1 beta antibody (AB106548)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

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