Rabbit Recombinant Monoclonal CCL4/MIP-1 beta antibody. Carrier free. Suitable for ICC/IF, Dot, Flow Cyt (Intra) and reacts with Human, Mouse, Synthetic peptide samples.
Images
![Flow Cytometry (Intracellular) - Anti-CCL4/MIP-1 beta antibody [EPR23610-40] - BSA and Azide free (AB275399), expandable thumbnail](https://content.abcam.com/products/images/ccl4-mip-1-beta-antibody-epr23610-40-bsa-and-azide-free-ab275399--flow-cytometry-intracellular-img78572.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry (Intracellular) - Anti-CCL4/MIP-1 beta antibody [EPR23610-40] - BSA and Azide free (AB275399)")
![Immunocytochemistry/ Immunofluorescence - Anti-CCL4/MIP-1 beta antibody [EPR23610-40] - BSA and Azide free (AB275399), expandable thumbnail](https://content.abcam.com/products/images/ccl4-mip-1-beta-antibody-epr23610-40-bsa-and-azide-free-ab275399--immunocytochemistry-immunofluorescence-img92956.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-CCL4/MIP-1 beta antibody [EPR23610-40] - BSA and Azide free (AB275399)")
![Dot Blot - Anti-CCL4/MIP-1 beta antibody [EPR23610-40] - BSA and Azide free (AB275399), expandable thumbnail](https://content.abcam.com/products/images/ccl4-mip-1-beta-antibody-epr23610-40-bsa-and-azide-free-ab275399--dot-blot-img4551.jpg/_jcr_content/renditions/webp-475.webp "Dot Blot - Anti-CCL4/MIP-1 beta antibody [EPR23610-40] - BSA and Azide free (AB275399)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Constituents: 100% PBS
- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| ICC/IF | IP | Dot | WB | IHC-P | Flow Cyt (Intra) | |
|---|---|---|---|---|---|---|
| Human | Tested | Not recommended | Expected | Not recommended | Not recommended | Tested |
| Mouse | Expected | Not recommended | Expected | Not recommended | Not recommended | Tested |
| Synthetic peptide | Not recommended | Not recommended | Tested | Not recommended | Not recommended | Not recommended |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Synthetic peptide | Dilution info - | Notes - |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Human, Synthetic peptide | Dilution info - | Notes - |
Dot
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Synthetic peptide | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
WB
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Human, Synthetic peptide | Dilution info - | Notes - |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Human, Synthetic peptide | Dilution info - | Notes - |
Flow Cyt (Intra)
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Human | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Synthetic peptide | Dilution info - | Notes - |
Associated Products
Select an associated product type
2 products for Alternative Version
Target data
Function
Monokine with inflammatory and chemokinetic properties. Binds to CCR5. One of the major HIV-suppressive factors produced by CD8+ T-cells. Recombinant MIP-1-beta induces a dose-dependent inhibition of different strains of HIV-1, HIV-2, and simian immunodeficiency virus (SIV). The processed form MIP-1-beta(3-69) retains the abilities to induce down-modulation of surface expression of the chemokine receptor CCR5 and to inhibit the CCR5-mediated entry of HIV-1 in T-cells. MIP-1-beta(3-69) is also a ligand for CCR1 and CCR2 isoform B.
Alternative names
LAG1, MIP1B, SCYA4, CCL4, C-C motif chemokine 4, G-26 T-lymphocyte-secreted protein, HC21, Lymphocyte activation gene 1 protein, MIP-1-beta(1-69), Macrophage inflammatory protein 1-beta, PAT 744, Protein H400, SIS-gamma, Small-inducible cytokine A4, T-cell activation protein 2, LAG-1, MIP-1-beta, ACT-2
Recommended products
Rabbit Recombinant Monoclonal CCL4/MIP-1 beta antibody. Carrier free. Suitable for ICC/IF, Dot, Flow Cyt (Intra) and reacts with Human, Mouse, Synthetic peptide samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Constituents: 100% PBS
- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR23610-40
- Purification technique
- Affinity purification Protein A
- Specificity
This antibody has cross-reactivity with CCL4L1. There is about 97% homology between CCL4/MIP-1 beta and CCL4L1 in terms of the immunogen designed from CCL4/MIP-1 beta.
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
Notes
ab275399 is the carrier-free version of Anti-CCL4/MIP-1 beta antibody [EPR23610-40] ab254371.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
CCL4 also known as MIP-1 beta or MIP-1b has a mechanical role as a chemokine involved in immune response regulation. The protein has a mass of about 7.8 kDa. It expresses in various tissues including leukocytes where it mainly functions as a chemoattractant. MIP-1 beta helps recruit immune cells like natural killer cells and monocytes to sites of inflammation or injury. Researchers frequently measure its levels using assays like MIP-1 beta ELISA or CCL4 ELISA.
- Biological function summary
CCL4 is critical in immune system modulation. It coordinates leukocyte activation and trafficking contributing to inflammation and immune surveillance. It does not function in isolation but rather interacts with other chemokines and receptors. CCL4 alongside its sibling chemokines forms a dynamic network that ensures a balanced immune response. It connects to receptors such as CCR5 playing substantial roles in its activities.
- Pathways
CCL4 participates in essential signaling cascades that mediate immune responses. It is notably part of the chemokine signaling pathway which is instrumental in directing cell movement. CCL4 also engages with proteins like CCR5 in the inflammatory and immune response pathways. This interaction enhances communication between cells required for effective pathogen defense.
- Associated diseases and disorders
CCL4 plays roles in immune-related conditions. It is strongly linked to inflammatory diseases such as rheumatoid arthritis and HIV infection. In rheumatoid arthritis CCL4 levels are often elevated correlating with inflammation severity. During HIV infection CCL4 interacts with proteins like CCR5 a co-receptor the virus uses to enter host cells. This involvement makes MIP-1 beta a target for studies seeking therapeutic interventions in these diseases.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
![Flow Cytometry (Intracellular) - Anti-CCL4/MIP-1 beta antibody [EPR23610-40] - BSA and Azide free (ab275399), expandable thumbnail](https://content.abcam.com/products/images/ccl4-mip-1-beta-antibody-epr23610-40-bsa-and-azide-free-ab275399--flow-cytometry-intracellular-img78572.jpg "Flow Cytometry (Intracellular) - Anti-CCL4/MIP-1 beta antibody [EPR23610-40] - BSA and Azide free (ab275399)")
Flow Cytometry (Intracellular) - Anti-CCL4/MIP-1 beta antibody [EPR23610-40] - BSA and Azide free (ab275399)
This data was developed using Anti-CCL4/MIP-1 beta antibody [EPR23610-40] ab254371, the same antibody clone in a different buffer formulation.
Intracellular flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol-permeabilized RAW 264.7 (mouse Abelson murine leukemia virus-induced tumor macrophage) (treated with 100 ng/ml lipopolysaccharide (LPS) for 4 hours and 1 ug/ml Brefeldin A (BFA) for another 3 hours) (Red) /Untreated control (Green) cells labelling CCL4/MIP-1 beta with Anti-CCL4/MIP-1 beta antibody [EPR23610-40] ab254371 at 1/50 dilution (1ug)/ compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) isotype control (Black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).
A Goat anti-rabbit IgG (Alexa Fluor®488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/2000 dilution was used as the secondary antibody.
The expression pattern is consistent with what is described in the literature (PMID:32230927).
![Immunocytochemistry/ Immunofluorescence - Anti-CCL4/MIP-1 beta antibody [EPR23610-40] - BSA and Azide free (ab275399), expandable thumbnail](https://content.abcam.com/products/images/ccl4-mip-1-beta-antibody-epr23610-40-bsa-and-azide-free-ab275399--immunocytochemistry-immunofluorescence-img92956.jpg "Immunocytochemistry/ Immunofluorescence - Anti-CCL4/MIP-1 beta antibody [EPR23610-40] - BSA and Azide free (ab275399)")
Immunocytochemistry/ Immunofluorescence - Anti-CCL4/MIP-1 beta antibody [EPR23610-40] - BSA and Azide free (ab275399)
This data was developed using Anti-CCL4/MIP-1 beta antibody [EPR23610-40] ab254371, the same antibody clone in a different buffer formulation.
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized THP-1 cells labelling CCL4/MIP-1 beta with Anti-CCL4/MIP-1 beta antibody [EPR23610-40] ab254371 at 1/100 (5.45 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 dilution (Green). Confocal image showing cytoplasmic staining in THP-1 cells treated with phorbol 12-myristate 13-acetate (PMA, 100nM) for 16 hours, then with lipopolysaccharide (LPS, 100 ng/ml) for 4 hours and with BFA (1 ug/ml) for another 3 hours. Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 (Red). The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution.
![Dot Blot - Anti-CCL4/MIP-1 beta antibody [EPR23610-40] - BSA and Azide free (ab275399), expandable thumbnail](https://content.abcam.com/products/images/ccl4-mip-1-beta-antibody-epr23610-40-bsa-and-azide-free-ab275399--dot-blot-img4551.jpg "Dot Blot - Anti-CCL4/MIP-1 beta antibody [EPR23610-40] - BSA and Azide free (ab275399)")
Dot Blot - Anti-CCL4/MIP-1 beta antibody [EPR23610-40] - BSA and Azide free (ab275399)
This data was developed using Anti-CCL4/MIP-1 beta antibody [EPR23610-40] ab254371, the same antibody clone in a different buffer formulation.
Dot blot analysis of CCL4/MIP-1 beta using Anti-CCL4/MIP-1 beta antibody [EPR23610-40] ab254371 at 1/1000 (0.545ug/ml) dilution followed by a Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20,000 dilution.
Lane 1: Human CCL4/MIP-1 beta immunogen recombinant protein
Lane 2: Human CCL4L1 recombinant protein
Lane 3: Bovine serum albumin (BSA)
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
Exposure time: 114 seconds
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