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Rabbit Recombinant Monoclonal CCL4/MIP-1 beta antibody. Suitable for IP, WB, ICC/IF and reacts with Human, Synthetic peptide - Human samples. Cited in 1 publication.

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Images

Immunoprecipitation - Anti-CCL4/MIP-1 beta + CCL4L1 antibody [EPR494] (AB40857), expandable thumbnail
  • Western blot - Anti-CCL4/MIP-1 beta + CCL4L1 antibody [EPR494] (AB40857), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-CCL4/MIP-1 beta + CCL4L1 antibody [EPR494] (AB40857), expandable thumbnail
  • Western blot - Anti-CCL4/MIP-1 beta + CCL4L1 antibody [EPR494] (AB40857), expandable thumbnail

Publications

Key facts

Isotype
IgG
Host species
Rabbit
Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form
Liquid
Clonality
Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
IPIHCWBICC/IF
Human
Tested
Not recommended
Tested
Tested
Synthetic peptide - Human
Not recommended
Not recommended
Tested
Not recommended

Tested
Tested

Species
Human
Dilution info
1/50
Notes

-

Not recommended
Not recommended

Species
Synthetic peptide - Human
Dilution info
-
Notes

-

Not recommended
Not recommended

Species
Human, Synthetic peptide - Human
Dilution info
-
Notes

-

Tested
Tested

Species
Synthetic peptide - Human
Dilution info
1/1000 - 1/500000
Notes

-

Species
Human
Dilution info
1/1000 - 1/500000
Notes

-

Tested
Tested

Species
Human
Dilution info
1/100
Notes

-

Not recommended
Not recommended

Species
Synthetic peptide - Human
Dilution info
-
Notes

-

Associated Products

Select an associated product type

4 products for Alternative Product

Target data

Function

Monokine with inflammatory and chemokinetic properties. Binds to CCR5. One of the major HIV-suppressive factors produced by CD8+ T-cells. Recombinant MIP-1-beta induces a dose-dependent inhibition of different strains of HIV-1, HIV-2, and simian immunodeficiency virus (SIV). The processed form MIP-1-beta(3-69) retains the abilities to induce down-modulation of surface expression of the chemokine receptor CCR5 and to inhibit the CCR5-mediated entry of HIV-1 in T-cells. MIP-1-beta(3-69) is also a ligand for CCR1 and CCR2 isoform B.

Additional Targets

CCL4L1

Alternative names

LAG1, MIP1B, SCYA4, CCL4, C-C motif chemokine 4, G-26 T-lymphocyte-secreted protein, HC21, Lymphocyte activation gene 1 protein, MIP-1-beta(1-69), Macrophage inflammatory protein 1-beta, PAT 744, Protein H400, SIS-gamma, Small-inducible cytokine A4, T-cell activation protein 2, LAG-1, MIP-1-beta, ACT-2

Recommended products

Rabbit Recombinant Monoclonal CCL4/MIP-1 beta antibody. Suitable for IP, WB, ICC/IF and reacts with Human, Synthetic peptide - Human samples. Cited in 1 publication.

Key facts

Isotype
IgG
Form
Liquid
Clonality
Monoclonal
Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number
EPR494
Purification technique
Affinity purification Protein A
Concentration
Loading...

Storage

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.
Activity summary

CCL4 also known as MIP-1 beta and CCL4L1 are chemokines involved in immune responses. These proteins weigh approximately 7.8 kDa. CCL4 and CCL4L1 are expressed in various tissues including lymphoid organs activated T cells and macrophages. Their expression occurs in response to inflammatory signals contributing to the attraction and activation of specific immune cells.

Biological function summary

The chemokines CCL4 and its homolog CCL4L1 play important roles in immune cell trafficking and coordination of the inflammatory response. They are involved in recruiting monocytes natural killer cells and various types of T cells to sites of inflammation. CCL4 and CCL4L1 function as part of a larger chemokine system interacting with receptors such as CCR5 to modulate immune responses effectively.

Pathways

The chemokines CCL4 and CCL4L1 feature prominently in the immune signaling pathway and the MAPK pathway. These pathways regulate immune responses and cell survival. Their interaction with receptor CCR5 links them to other chemokines like CCL3 which also utilizes CCR5. This signaling cascade influences cellular responses essential for proper immune system functioning.

Associated diseases and disorders

CCL4 and CCL4L1 play roles in HIV infection and rheumatoid arthritis. They affect HIV progression by binding to the CCR5 receptor which the virus uses to enter host cells. In rheumatoid arthritis they contribute to synovial inflammation through recruitment of inflammatory cells. These interactions highlight their connection with other proteins like HIV-1 gp120 and CCR5 in HIV and TNF-alpha in rheumatoid arthritis.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

4 product images

  • Immunoprecipitation - Anti-CCL4/MIP-1 beta + CCL4L1 antibody [EPR494] (ab40857), expandable thumbnail

    Immunoprecipitation - Anti-CCL4/MIP-1 beta + CCL4L1 antibody [EPR494] (ab40857)

    ab40857 at 1/50 immunoprecipitating CCL4/MIP-1 beta/CCL4 + CCL4L in THP-1 (Human monocytic leukemia cell line) whole cell lysate observed at 12 KDa (lanes 1 and 2).

    Lane 1 (input): THP-1 treated with 100 nM PMA overnight, then treated with 100 ng/mL LPS for 7 hours and 1 μg/mL Brefeldin A was added for the last 3 hours whole cell lysate, 10μg

    Lane 2 (+): ab40857 + THP-1 treated with 100 nM PMA overnight, then treated with 100 ng/mL LPS for 7 hours and 1μg/mL Brefeldin A was added for the last 3 hours whole cell lysate

    Lane 3 (-): Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of ab40857 in THP-1 treated with 100 nM PMA overnight, then treated with 100 ng/mL LPS for 7 hours and 1 μg/mL Brefeldin A was added for the last 3 hours whole cell lysate

    For western blotting: ab40857 at 1/1000 followed by VeriBlot for IP Detection Reagent (HRP) ab131366 VeriBlot for IP Detection Reagent (HRP) for detection at 1/000.

    Blocking buffer and concentration: 5% NFDM/TBST.
    Diluting buffer and concentration: 5% NFDM /TBST.

    All lanes: Immunoprecipitation - Anti-CCL4/MIP-1 beta + CCL4L1 antibody [EPR494] (ab40857)

    Exposure time: 10s

  • Western blot - Anti-CCL4/MIP-1 beta + CCL4L1 antibody [EPR494] (ab40857), expandable thumbnail

    Western blot - Anti-CCL4/MIP-1 beta + CCL4L1 antibody [EPR494] (ab40857)

    Blocking/Diluting buffer 5% NFDM /TBST.

    CCL4/MIP-1 beta (CCL4) is induced in macrophages following exposure to bacterial LPS (PMID: 9848081).

    All lanes: Western blot - Anti-CCL4/MIP-1 beta + CCL4L1 antibody [EPR494] (ab40857) at 1/1000 dilution

    Lane 1: Untreated THP-1 (human acute monocytic leukemia) lysate at 10 µg

    Lane 2: THP-1 (human acute monocytic leukemia) treated with 100 nM Phorbol-12-myristate-13-acetate(PMA) overnight, then treated with Lipopolysaccharides (LPS) 100 ng/mL for 7 hours and then 1 μg/mL Brefeldin A was added for the last 3 hours, lysate at 10 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Observed band size: 12 kDa

    Exposure time: 15s

  • Immunocytochemistry/ Immunofluorescence - Anti-CCL4/MIP-1 beta + CCL4L1 antibody [EPR494] (ab40857), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-CCL4/MIP-1 beta + CCL4L1 antibody [EPR494] (ab40857)

    Immunocytochemistry/Immunofluorescence analysis of THP-1 (Human monocytic leukemia cell line) labeling CCL4/MIP-1 beta/CCL4 + CCL4L with ab40857 at 1/100. Cells were fixed with 4% Paraformaldehyde and permeabilized with 0.1% Triton X-100. Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077, Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody. Cells were counter-stained with Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889, Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) at 1/200. Nuclei were counterstained with DAPI (blue).

    The expression increased after treatment with Lipopolysaccharides (LPS), 100 ng/mL for 4 hours, followed by addition of Brefeldin A (1 μg/mL) for 3 hours.

  • Western blot - Anti-CCL4/MIP-1 beta + CCL4L1 antibody [EPR494] (ab40857), expandable thumbnail

    Western blot - Anti-CCL4/MIP-1 beta + CCL4L1 antibody [EPR494] (ab40857)

    Blocking/Diluting buffer 5% NFDM /TBST

    All lanes: Western blot - Anti-CCL4/MIP-1 beta + CCL4L1 antibody [EPR494] (ab40857) at 1/10000 dilution

    Lane 1: Untagged human CCL4 recombinant protein (aa24-92) at 10 µg

    Lane 2: Untagged human CCL4L recombinant protein (aa24-92) at 10 µg

    Lane 3: GST-tagged human CCL3 recombinant protein (aa27-92) at 10 µg

    Lane 4: GST-tagged human CCL3L recombinant protein 2*(aa28-93) at 10 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution

    Observed band size: 12 kDa

    Exposure time: 3s

Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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