AB40857

Anti-CCL4/MIP-1 beta + CCL4L1 antibody [EPR494]

RabMAb
Recombinant
What is this?

Be the first to review this product! Submit a review

(2 Publications)

Rabbit Recombinant Monoclonal CCL4/MIP-1 beta antibody. Suitable for IP, WB, ICC/IF and reacts with Human, Synthetic peptide - Human samples. Cited in 2 publications.

View Alternative Names

LAG1, MIP1B, SCYA4, CCL4, C-C motif chemokine 4, G-26 T-lymphocyte-secreted protein, HC21, Lymphocyte activation gene 1 protein, MIP-1-beta(1-69), Macrophage inflammatory protein 1-beta, PAT 744, Protein H400, SIS-gamma, Small-inducible cytokine A4, T-cell activation protein 2, LAG-1, MIP-1-beta, ACT-2

4 Images

Immunocytochemistry/ Immunofluorescence - Anti-CCL4/MIP-1 beta + CCL4L1 antibody [EPR494] (AB40857)

ICC/IF

Lab

Immunocytochemistry/ Immunofluorescence - Anti-CCL4/MIP-1 beta + CCL4L1 antibody [EPR494] (AB40857)

Immunocytochemistry/Immunofluorescence analysis of THP-1 (Human monocytic leukemia cell line) labeling CCL4/MIP-1 beta/CCL4 + CCL4L with ab40857 at 1/100. Cells were fixed with 4% Paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150077, Alexa Fluor^® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody. Cells were counter-stained with ab195889, Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor^® 594) at 1/200. Nuclei were counterstained with DAPI (blue).

The expression increased after treatment with Lipopolysaccharides (LPS), 100 ng/mL for 4 hours, followed by addition of Brefeldin A (1 μg/mL) for 3 hours.

Unknown

Immunoprecipitation - Anti-CCL4/MIP-1 beta + CCL4L1 antibody [EPR494] (AB40857)

ab40857 at 1/50 immunoprecipitating CCL4/MIP-1 beta/CCL4 + CCL4L in THP-1 (Human monocytic leukemia cell line) whole cell lysate observed at 12 KDa (lanes 1 and 2).

Lane 1 (input) : THP-1 treated with 100 nM PMA overnight, then treated with 100 ng/mL LPS for 7 hours and 1 μg/mL Brefeldin A was added for the last 3 hours whole cell lysate, 10μg

Lane 2 (+) : ab40857 + THP-1 treated with 100 nM PMA overnight, then treated with 100 ng/mL LPS for 7 hours and 1μg/mL Brefeldin A was added for the last 3 hours whole cell lysate

Lane 3 (-) : Rabbit monoclonal IgG (ab172730) instead of ab40857 in THP-1 treated with 100 nM PMA overnight, then treated with 100 ng/mL LPS for 7 hours and 1 μg/mL Brefeldin A was added for the last 3 hours whole cell lysate

For western blotting : ab40857 at 1/1000 followed by ab131366 VeriBlot for IP Detection Reagent (HRP) for detection at 1/000.

Blocking buffer and concentration : 5% NFDM/TBST.
Diluting buffer and concentration : 5% NFDM /TBST.

All lanes:

Immunoprecipitation - Anti-CCL4/MIP-1 beta + CCL4L1 antibody [EPR494] (ab40857)

false

Exposure time: 10s

Lab

Western blot - Anti-CCL4/MIP-1 beta + CCL4L1 antibody [EPR494] (AB40857)

Blocking/Diluting buffer 5% NFDM /TBST.

CCL4/MIP-1 beta (CCL4) is induced in macrophages following exposure to bacterial LPS (PMID : 9848081).

All lanes:

Western blot - Anti-CCL4/MIP-1 beta + CCL4L1 antibody [EPR494] (ab40857) at 1/1000 dilution

Lane 1:

Untreated THP-1 (human acute monocytic leukemia) lysate at 10 µg

Lane 2:

THP-1 (human acute monocytic leukemia) treated with 100 nM Phorbol-12-myristate-13-acetate(PMA) overnight, then treated with Lipopolysaccharides (LPS) 100 ng/mL for 7 hours and then 1 μg/mL Brefeldin A was added for the last 3 hours, lysate at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 12 kDa

false

Exposure time: 15s

Lab

Western blot - Anti-CCL4/MIP-1 beta + CCL4L1 antibody [EPR494] (AB40857)

Blocking/Diluting buffer 5% NFDM /TBST

All lanes:

Western blot - Anti-CCL4/MIP-1 beta + CCL4L1 antibody [EPR494] (ab40857) at 1/10000 dilution

Lane 1:

Untagged human CCL4 recombinant protein (aa24-92) at 10 µg

Lane 2:

Untagged human CCL4L recombinant protein (aa24-92) at 10 µg

Lane 3:

GST-tagged human CCL3 recombinant protein (aa27-92) at 10 µg

Lane 4:

GST-tagged human CCL3L recombinant protein 2*(aa28-93) at 10 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/100000 dilution

Observed band size: 12 kDa

false

Exposure time: 3s

Carrier free

Anti-CCL4/MIP-1 beta + CCL4L1 antibody [EPR494] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR494

Isotype

IgG

Carrier free

Reacts with

Human

Applications

IP, ICC/IF, WB

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

style

left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "IHC" : {"fullname" : "Immunohistochemistry", "shortname":"IHC"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "1/50", "IP-species-notes": "", "IHC-species-checked": "notRecommended", "IHC-species-dilution-info": "", "IHC-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/500000", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "" }, "Synthetic peptide - Human": { "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "IHC-species-checked": "notRecommended", "IHC-species-dilution-info": "", "IHC-species-notes": "", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/1000 - 1/500000", "WB-species-notes": "", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

style

left-column

Product details

Patented technology
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

style

left-column

Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

style

left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CCL4 also known as MIP-1 beta and CCL4L1 are chemokines involved in immune responses. These proteins weigh approximately 7.8 kDa. CCL4 and CCL4L1 are expressed in various tissues including lymphoid organs activated T cells and macrophages. Their expression occurs in response to inflammatory signals contributing to the attraction and activation of specific immune cells.

Biological function summary

The chemokines CCL4 and its homolog CCL4L1 play important roles in immune cell trafficking and coordination of the inflammatory response. They are involved in recruiting monocytes natural killer cells and various types of T cells to sites of inflammation. CCL4 and CCL4L1 function as part of a larger chemokine system interacting with receptors such as CCR5 to modulate immune responses effectively.

Pathways

The chemokines CCL4 and CCL4L1 feature prominently in the immune signaling pathway and the MAPK pathway. These pathways regulate immune responses and cell survival. Their interaction with receptor CCR5 links them to other chemokines like CCL3 which also utilizes CCR5. This signaling cascade influences cellular responses essential for proper immune system functioning.

CCL4 and CCL4L1 play roles in HIV infection and rheumatoid arthritis. They affect HIV progression by binding to the CCR5 receptor which the virus uses to enter host cells. In rheumatoid arthritis they contribute to synovial inflammation through recruitment of inflammatory cells. These interactions highlight their connection with other proteins like HIV-1 gp120 and CCR5 in HIV and TNF-alpha in rheumatoid arthritis.

style

left-column

style

left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

style

left-column

Target data

Monokine with inflammatory and chemokinetic properties. Binds to CCR5. One of the major HIV-suppressive factors produced by CD8+ T-cells. Recombinant MIP-1-beta induces a dose-dependent inhibition of different strains of HIV-1, HIV-2, and simian immunodeficiency virus (SIV). The processed form MIP-1-beta(3-69) retains the abilities to induce down-modulation of surface expression of the chemokine receptor CCR5 and to inhibit the CCR5-mediated entry of HIV-1 in T-cells. MIP-1-beta(3-69) is also a ligand for CCR1 and CCR2 isoform B.

See full target information CCL4

Additional targets

CCL4L1

style

left-column

Publications (2)

Recent publications for all applications. Explore the full list and refine your search

BMC nephrology 26:526 PubMed41013408

2025

INHBA knockdown inhibits renal fibrosis in mice following ischemia-reperfusion injury by suppressing activation of the TGF-β/Smad signaling pathway.

Applications

Unspecified application

Species

Unspecified reactive species

Yifei Wang,Qiao Tang,Qian Sun

PloS one 18:e0287829 PubMed37910521

2023

Interleukin-27-induced HIV-resistant dendritic cells suppress reveres transcription following virus entry in an SPTBN1, autophagy, and YB-1 independent manner.

Applications

Unspecified application

Species

Unspecified reactive species

Tomozumi Imamichi,Qian Chen,Bharatwaj Sowrirajan,Jun Yang,Sylvain Laverdure,Mayra Marquez,Anthony R Mele,Catherine Watkins,Joseph W Adelsberger,Jeanette Higgins,Hongyan Sui

View all publications

style

left-column

style

left-column

style

right-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-CCL4/MIP-1 beta + CCL4L1 antibody [EPR494]

Immunocytochemistry/ Immunofluorescence - Anti-CCL4/MIP-1 beta + CCL4L1 antibody [EPR494] (AB40857)

Immunoprecipitation - Anti-CCL4/MIP-1 beta + CCL4L1 antibody [EPR494] (AB40857)

Western blot - Anti-CCL4/MIP-1 beta + CCL4L1 antibody [EPR494] (AB40857)

Western blot - Anti-CCL4/MIP-1 beta + CCL4L1 antibody [EPR494] (AB40857)

Related conjugates and formulations (1)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Additional targets

Publications (2)

Alternative Products

Proteins & Peptides

Primary Antibodies

Primary Antibodies

Primary Antibodies

Complementary Products

Assay Kits

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Product promise