AB129386

Anti-CCM2 antibody

Name: Anti-CCM2 antibody (ab129386) | Abcam
Brand: Abcam Limited
SKU: AB129386
Price: 520 USD
Availability: InStock

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(1 Publication)

Rabbit Polyclonal CCM2 antibody. Carrier free. Suitable for WB, ICC/IF and reacts with Transfected cell lysate, Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human CCM2.

View Alternative Names

C7orf22, PP10187, CCM2, Cerebral cavernous malformations 2 protein, Malcavernin

3 Images

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Western blot - Anti-CCM2 antibody (AB129386)

All lanes:

Western blot - Anti-CCM2 antibody (ab129386) at 5 µg/mL

Lane 1:

Mwt marker

Lane 2:

CCM2 knockout

Lane 3:

CCM2 wildtype

Lane 4:

CCM2 overexpressed in C0S1 cells

Predicted band size: 49 kDa

false

Unknown

Western blot - Anti-CCM2 antibody (AB129386)

All lanes:

Western blot - Anti-CCM2 antibody (ab129386) at 5 µg/mL

Lane 1:

Mwt marker

Lane 2:

CCM2 knockout

Lane 3:

CCM2 wildtype

Lane 4:

CCM2 overexpressed in C0S1 cells

Predicted band size: 49 kDa

false

Immunocytochemistry/ Immunofluorescence - Anti-CCM2 antibody (AB129386)

ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-CCM2 antibody (AB129386)

ab129386, at a 1/50 dilution, staining CCM2 (green) in a cryo-section of unfixed Human foreskin tissue by Immunofluorescence.
A) Note specific staining in the wall of microvessels.
B) Negative control of a consecutive section.
Note non-specific fluorescence in elastic fibres in the adventitia of an arteriol.
Nuclei counter-stained with Dapi (blue).

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

WB, ICC/IF

applications

Immunogen

Recombinant Full Length Protein corresponding to Human CCM2.

Q9BSQ5

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/200", "ICCIF-species-notes": "<p></p>" }, "Transfected cell lysate": { "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "notRecommended", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Purification notes

Endotoxin level: <0.1 EU/1μg of ab129386 (LAL)

Storage buffer

Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CCM2 also known as Malcavernin functions in the stability of cell-cell junctions in blood vessels. It has an approximate molecular mass of 50 kDa. The protein is largely expressed in vascular endothelial cells which are cells lining the inside of the blood vessels. CCM2 forms part of a protein scaffold that guides the cellular responses to mechanical stress maintaining vascular integrity.

Biological function summary

It interacts with several proteins to form a well-coordinated complex including KRIT1 and CCM3 contributing to the maintenance of vascular structure. The CCM2 protein is essential for regulating the cellular pathways that respond to oxidative stress. It facilitates actin cytoskeleton organization and modulates endothelial barrier function providing a structural basis for cellular processes involving vascular homeostasis.

Pathways

The protein is an important component in the RhoA signaling and MAPK pathways which influence cellular shape and proliferation. Within these pathways CCM2 influences various proteins including ROCK and MEK to execute its function. It ensures proper signal transduction that is necessary for maintaining cellular responses to environmental changes which can be important for normal vascular function.

CCM2 mutations associate closely with cerebral cavernous malformations (CCM) which trigger abnormal blood vessel formation in the brain that leads to seizures or stroke. During this condition the protein's interaction with KRIT1 exhibits significant influence since defects in the gene encoding CCM2 or KRIT1 can lead to disease phenotypes. Researchers study these interactions to better understand and develop therapeutics for vascular malformation-related diseases.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Component of the CCM signaling pathway which is a crucial regulator of heart and vessel formation and integrity. May act through the stabilization of endothelial cell junctions (By similarity). May function as a scaffold protein for MAP2K3-MAP3K3 signaling. Seems to play a major role in the modulation of MAP3K3-dependent p38 activation induced by hyperosmotic shock (By similarity).

See full target information CCM2

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Pigment cell & melanoma research 30:317-327 PubMed28140525

2017

Nanobodies against surface biomarkers enable the analysis of tumor genetic heterogeneity in uveal melanoma patient-derived xenografts.

Applications

Unspecified application

Species

Unspecified reactive species

Ronan Crépin,David Gentien,Angeline Duché,Audrey Rapinat,Cecile Reyes,Fariba Némati,Gérald Massonnet,Didier Decaudin,Selma Djender,Sandrine Moutel,Klervi Desrumeaux,Nathalie Cassoux,Sophie Piperno-Neumann,Sebastian Amigorena,Franck Perez,Sergio Roman-Roman,Ario de Marco

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-CCM2 antibody

Western blot - Anti-CCM2 antibody (AB129386)

Western blot - Anti-CCM2 antibody (AB129386)

Immunocytochemistry/ Immunofluorescence - Anti-CCM2 antibody (AB129386)

Key facts

Host species

Clonality

Isotype

Carrier free

Reacts with

Applications

Immunogen

Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

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