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AB123930

Anti-CCM2 antibody [OTI2E4]

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(1 Publication)

Mouse Monoclonal CCM2 antibody. Suitable for IHC-P, WB, ICC/IF and reacts with Human, African green monkey samples. Cited in 1 publication. Immunogen corresponding to Recombinant Full Length Protein corresponding to Human CCM2.

View Alternative Names

C7orf22, PP10187, CCM2, Cerebral cavernous malformations 2 protein, Malcavernin

3 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CCM2 antibody [OTI2E4] (AB123930)
  • IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CCM2 antibody [OTI2E4] (AB123930)

Paraffin-embedded human kidney tissue stained for CCM2 with ab123930 at a 1/150 dilution in immunohistochemical analysis. Heat-induced epitope retrieval by 10 mM citric buffer, pH 6.0, 100°C for 10 minutes.

Immunocytochemistry/ Immunofluorescence - Anti-CCM2 antibody [OTI2E4] (AB123930)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-CCM2 antibody [OTI2E4] (AB123930)

pCMV6-ENTRY CCM2 cDNA transfected COS-7 (African green monkey kidney fibroblast-like cell line) cells stained for CCM2 using ab123930 at a 1/100 dilution in ICC/IF.

Western blot - Anti-CCM2 antibody [OTI2E4] (AB123930)
  • WB

Supplier Data

Western blot - Anti-CCM2 antibody [OTI2E4] (AB123930)

All lanes:

Western blot - Anti-CCM2 antibody [OTI2E4] (ab123930) at 1/2000 dilution

Lane 1:

HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) cells transfected with pCMV6-ENTRY control cDNA at 5 µg

Lane 2:

HEK-293T cells transfected with pCMV6-ENTRY CCM2 cDNA at 5 µg

Predicted band size: 49 kDa

false

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

OTI2E4

Isotype

IgG1

Carrier free

No

Reacts with

Human, African green monkey

Applications

ICC/IF, IHC-P, WB

applications

Immunogen

Recombinant Full Length Protein corresponding to Human CCM2.

Q9BSQ5

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/150", "IHCP-species-notes": "<p></p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/2000", "WB-species-notes": "<p></p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "African green monkey": { "IHCP-species-checked": "guaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "", "WB-species-checked": "guaranteed", "WB-species-dilution-info": "", "WB-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/100", "ICCIF-species-notes": "<p></p>" } } }
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Product details

Clone OTI2E4 (formerly 2E4).
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Purification notes
Purified from cell culture supernatant by affinity chromatography.
Storage buffer
pH: 7.3 Preservative: 0.02% Sodium azide Constituents: PBS, 50% Glycerol (glycerin, glycerine), 1% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CCM2 also known as Malcavernin functions in the stability of cell-cell junctions in blood vessels. It has an approximate molecular mass of 50 kDa. The protein is largely expressed in vascular endothelial cells which are cells lining the inside of the blood vessels. CCM2 forms part of a protein scaffold that guides the cellular responses to mechanical stress maintaining vascular integrity.
Biological function summary

It interacts with several proteins to form a well-coordinated complex including KRIT1 and CCM3 contributing to the maintenance of vascular structure. The CCM2 protein is essential for regulating the cellular pathways that respond to oxidative stress. It facilitates actin cytoskeleton organization and modulates endothelial barrier function providing a structural basis for cellular processes involving vascular homeostasis.

Pathways

The protein is an important component in the RhoA signaling and MAPK pathways which influence cellular shape and proliferation. Within these pathways CCM2 influences various proteins including ROCK and MEK to execute its function. It ensures proper signal transduction that is necessary for maintaining cellular responses to environmental changes which can be important for normal vascular function.

CCM2 mutations associate closely with cerebral cavernous malformations (CCM) which trigger abnormal blood vessel formation in the brain that leads to seizures or stroke. During this condition the protein's interaction with KRIT1 exhibits significant influence since defects in the gene encoding CCM2 or KRIT1 can lead to disease phenotypes. Researchers study these interactions to better understand and develop therapeutics for vascular malformation-related diseases.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Component of the CCM signaling pathway which is a crucial regulator of heart and vessel formation and integrity. May act through the stabilization of endothelial cell junctions (By similarity). May function as a scaffold protein for MAP2K3-MAP3K3 signaling. Seems to play a major role in the modulation of MAP3K3-dependent p38 activation induced by hyperosmotic shock (By similarity).
See full target information CCM2
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

The Journal of biological chemistry 287:29285-9 PubMed22782892

2012

STK25 protein mediates TrkA and CCM2 protein-dependent death in pediatric tumor cells of neural origin.

Applications

Unspecified application

Species

Unspecified reactive species

Barbara Costa,Michelle J Kean,Volker Ast,James D R Knight,Alice Mett,Zehava Levy,Derek F Ceccarelli,Beatriz Gonzalez Badillo,Roland Eils,Rainer König,Anne-Claude Gingras,Mike Fainzilber
View all publications
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Product promise

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