Rabbit Polyclonal CCNDBP1 antibody. Suitable for IHC-P, WB and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human CCNDBP1 aa 250 to C-terminus.
Images
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine- Form
Liquid
- Clonality
Polyclonal
Immunogen
- Synthetic Peptide within Human CCNDBP1 aa 250 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link O95273
Reactivity data
Select an application| IHC-P | WB | |
|---|---|---|
| Human | Tested | Tested |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/100.00000 - 1/1000.00000 | Notes Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/500.00000 - 1/3000.00000 | Notes - |
Associated Products
Select an associated product type
1 products for Alternative Product
Target data
Function
May negatively regulate cell cycle progression. May act at least in part via inhibition of the cyclin-D1/CDK4 complex, thereby preventing phosphorylation of RB1 and blocking E2F-dependent transcription.
Alternative names
Cyclin-D1-binding protein 1, Grap2 and cyclin-D-interacting protein, Human homolog of Maid, HHM, GCIP, DIP1, CCNDBP1
Recommended products
Rabbit Polyclonal CCNDBP1 antibody. Suitable for IHC-P, WB and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human CCNDBP1 aa 250 to C-terminus.
Alternative names
Cyclin-D1-binding protein 1, Grap2 and cyclin-D-interacting protein, Human homolog of Maid, HHM, GCIP, DIP1, CCNDBP1
Key facts
- Isotype
IgG
- Host species
Rabbit
- Storage buffer
pH: 7
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 10% Glycerol (glycerin, glycerine), 1.21% Tris, 0.75% Glycine- Form
Liquid
- Clonality
Polyclonal
- Immunogen
- Synthetic Peptide within Human CCNDBP1 aa 250 to C-terminus. The exact immunogen used to generate this antibody is proprietary information. Database link O95273
- Purification technique
Affinity purification Immunogen
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate long-term storage conditions
-20°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Notes
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
CCNDBP1 also known as Cyclin D1 binding protein 1 is a protein that has a molecular mass of approximately 38 kDa. It performs critical mechanical functions by binding to cyclin D1 and affects the regulation of the cell cycle. This protein is widely expressed in multiple tissues but is especially found in organs such as the liver kidney and heart. Its presence in these organs indicates its important role across various functional domains within the body.
- Biological function summary
CCNDBP1 shows involvement in cell cycle regulation and modulation of transcription activities. It functions as a negative regulator of cell proliferation. Despite not being part of any large protein complexes it does influence gene expression through its interaction with cyclin D1 which in turn adjusts the progression of the cell cycle. Its activity suggests a role in controlling the timely and orderly progression of various cell cycle phases.
- Pathways
CCNDBP1 is an important player in cell cycle and transcription regulation. It significantly influences the Cyclin D1-CDK4/6 pathway which is important for cell cycle G1/S transition. The interaction with cyclin D1 links CCNDBP1 to the MAPK/ERK signaling pathway highlighting its importance in signal transduction processes and stress response. These connections suggest it indirectly interacts with other proteins such as CDK4 and CDK6 through its regulatory effects on cyclin D1.
- Associated diseases and disorders
Researchers have linked CCNDBP1 to certain cancers like hepatocellular carcinoma and breast cancer. Its role as a negative regulator of cyclin D1 impacts tumor progression and proliferation. In hepatocellular carcinoma CCNDBP1 exhibits downregulated expression. Similarly in breast cancer disruptions in cyclin D1 modulation caused by CCNDBP1 can lead to uncontrolled cell growth. This connection suggests that CCNDBP1 through cyclin D1 plays a potential role in cancer biology making it a target for therapeutic investigations.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
Western blot - Anti-CCNDBP1 antibody - C-terminal (ab153964)
12% SDS PAGE
All lanes: Western blot - Anti-CCNDBP1 antibody - C-terminal (ab153964) at 1/500 dilution
Lane 1: 293T lysate, non-transfected
Lane 2: 293T lysate, transfected with CCNDBP1
Predicted band size: 40 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CCNDBP1 antibody - C-terminal (ab153964)
Immunohistochemical analysis of paraffin-embedded Human endomitrial tissue labeling CCNDBP1 with ab153964 at 1/100 dilution.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com