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AB244512

Anti-CCP1 antibody

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(1 Publication)

Rabbit Polyclonal CCP1 antibody. Suitable for IHC-P, ICC/IF and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Recombinant Fragment Protein within Human AGTPBP1 aa 1000 to C-terminus.

View Alternative Names

CCP1, KIAA1035, NNA1, AGTPBP1, Cytosolic carboxypeptidase 1, ATP/GTP-binding protein 1, Nervous system nuclear protein induced by axotomy protein 1 homolog, Protein deglutamylase CCP1

2 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CCP1 antibody (AB244512)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CCP1 antibody (AB244512)

Paraffin-embedded human testis tissue stained for CCP1 using ab244512 at 1/200 dilution in immunohistochemical analysis.

Immunocytochemistry/ Immunofluorescence - Anti-CCP1 antibody (AB244512)
  • ICC/IF

Unknown

Immunocytochemistry/ Immunofluorescence - Anti-CCP1 antibody (AB244512)

PFA-fixed, Triton X-100 permeabilized U-2 OS (human bone osteosarcoma epithelial cell line) cells stained for CCP1 (green) using ab244512 at 4 μg/ml in ICC/IF.

Key facts

Host species

Rabbit

Clonality

Polyclonal

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

IHC-P, ICC/IF

applications

Immunogen

Recombinant Fragment Protein within Human AGTPBP1 aa 1000 to C-terminus. The exact immunogen used to generate this antibody is proprietary information.

Q9UPW5

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/200 - 1/500", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "0.25-2 µg/mL", "ICCIF-species-notes": "<p>Fixation/Permeabilization: PFA/Triton X-100.</p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Immunogen
Storage buffer
pH: 7.2 Preservative: 0.02% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine)
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CCP1 also known as Cytosolic Carboxypeptidase 1 is an enzymatic protein important in the modulation of microtubule function. This protein is approximately 76 kDa in mass and is mainly expressed in the brain and testes but it can be found in other tissues as well. CCP1 acts by trimming the C-terminal amino acids of tubulin and other substrates impacting their stability and function. This modification plays a role in determining microtubule dynamics which are important for cell shape division and intracellular transport.
Biological function summary

CCP1 contributes to maintaining cellular functions by removing glutamates from the C-terminal of tubulin impacting microtubule behavior. It forms part of the tubulin tyrosination-detyrosination cycle and interacts with other proteins within the cycle to regulate microtubule length and stability. This regulation is essential for the proper operation of neuronal cells and can influence processes like axonal transport and signaling.

Pathways

CCP1's enzymatic activity links to microtubule and cellular cytoskeleton organization pathways. It influences the dynamic instability of microtubules essential in cell division and intracellular traffic. CCP1 interacts with other tubulin-modifying enzymes like CCP5 which together modify microtubule attributes through polyglutamylation and serve roles in cell motility and structural integrity.

CCP1 alterations or mutations have associations with neurodegenerative disorders and certain forms of cancer. Its dysfunction can lead to the progressive degeneration of neurons connected to Ataxia a condition affecting motor control. Additionally CCP1 mutations or disruptions can link to tubulin-binding proteins contributing to unregulated cell proliferation seen in some cancers. Understanding CCP1's role in these conditions can guide therapeutic strategies targeting these proteins and pathways.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Metallocarboxypeptidase that mediates protein deglutamylation of tubulin and non-tubulin target proteins (PubMed : 22170066, PubMed : 24022482, PubMed : 30420557). Catalyzes the removal of polyglutamate side chains present on the gamma-carboxyl group of glutamate residues within the C-terminal tail of alpha- and beta-tubulin (PubMed : 22170066, PubMed : 24022482, PubMed : 30420557). Specifically cleaves tubulin long-side-chains, while it is not able to remove the branching point glutamate (PubMed : 24022482). Also catalyzes the removal of polyglutamate residues from the carboxy-terminus of alpha-tubulin as well as non-tubulin proteins such as MYLK (PubMed : 22170066). Involved in KLF4 deglutamylation which promotes KLF4 proteasome-mediated degradation, thereby negatively regulating cell pluripotency maintenance and embryogenesis (PubMed : 29593216).
See full target information AGTPBP1
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Viruses 17: PubMed40431628

2025

The COPII Transport Complex Participates in HPV16 Infection.

Applications

Unspecified application

Species

Unspecified reactive species

Patricia M Day,Cynthia D Thompson,Andrea S Weisberg,John T Schiller
View all publications
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Product promise

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