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AB276049

Anti-CCR1 antibody [002] - BSA and Azide free

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Rabbit Recombinant Monoclonal CCR1 antibody. Carrier free. Suitable for IP, WB and reacts with Human samples. Immunogen corresponding to Synthetic Peptide within Human CCR1.

View Alternative Names

CD191, CMKBR1, CMKR1, SCYAR1, CCR1, C-C chemokine receptor type 1, C-C CKR-1, CC-CKR-1, CCR-1, HM145, LD78 receptor, Macrophage inflammatory protein 1-alpha receptor, RANTES-R, MIP-1alpha-R

2 Images
Immunoprecipitation - Anti-CCR1 antibody [002] - BSA and Azide free (AB276049)
  • IP

Unknown

Immunoprecipitation - Anti-CCR1 antibody [002] - BSA and Azide free (AB276049)

CCR1/CD191 was immunoprecipitated from 0.5 mg of HeLa (human epithelial cell line from cervix adenocarcinoma) (lane A) and HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) (lane B) whole cell lysate with 2 μL ab276049 and 15 μl of 50% Protein G agarose. Western blot was performed from the immunoprecipitate using ab276049 at 1/100 dilution. Rabbit IgG (H+L) Dylight 800-labeled antibody, was used as secondary antibodyat 1/5000 dilution.

Developed using the odssey technique.
Performed under reducing conditions.

All lanes:

Immunoprecipitation - Anti-CCR1 antibody [002] - BSA and Azide free (ab276049)

Predicted band size: 41 kDa

false

Western blot - Anti-CCR1 antibody [002] - BSA and Azide free (AB276049)
  • WB

Unknown

Western blot - Anti-CCR1 antibody [002] - BSA and Azide free (AB276049)

Developed using the Odyssey technique.
Performed under reducing conditions.

All lanes:

Western blot - Anti-CCR1 antibody [002] - BSA and Azide free (ab276049) at 1/500 dilution

Lane 1:

HEK-293 (human epithelial cell line from embryonic kidney) whole cell lysate at 30 µg

Lane 2:

HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 30 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution

Predicted band size: 41 kDa

false

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

002

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

IP, WB

applications

Immunogen

Synthetic Peptide within Human CCR1. The exact immunogen used to generate this antibody is proprietary information.

P32246

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IP-species-checked": "testedAndGuaranteed", "IP-species-dilution-info": "", "IP-species-notes": "<p>1-4 μL/mg of lysate</p>", "WB-species-checked": "testedAndGuaranteed", "WB-species-dilution-info": "1/500 - 1/2000", "WB-species-notes": "<p></p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The target CCR1 also known as C-C chemokine receptor type 1 plays a mechanical role in mediating cellular responses to chemokines. It is a protein with a molecular mass of approximately 45 kDa. CCR1 expresses on a variety of cells including immune cells like monocytes macrophages and certain lymphocytes as well as some non-hematopoietic cells. This receptor resides on the cell membrane and contributes to the directed movement of these cells in response to chemokine gradients.
Biological function summary

CCR1 involves itself in processes such as leukocyte migration and inflammatory response. The receptor interacts with specific chemokines of the C-C motif like CCL3 and CCL5. CCR1 does not operate within a higher-order protein complex but binds directly to these chemokines leading to signal transduction that alters cell functions. By modulating immune cell behaviors CCR1 helps orchestrate immune surveillance and inflammation.

Pathways

CCR1 plays a significant part in immune-related pathways such as chemokine signaling and the inflammatory response. It transduces signals via G-protein coupled receptor pathways influencing downstream signaling molecules including PI3K and AKT. Apart from its direct chemokine interactions CCR1 regulates functions in concert with proteins like CCR5 another chemokine receptor coordinating the migration and activation of immune cells across tissues.

CCR1 contributes to conditions such as rheumatoid arthritis and multiple sclerosis. Its role in directing immune cell trafficking makes it a potential player in autoimmune inflammation observed in these diseases. CCR1's interaction with other proteins such as CCR5 and their combined roles in these conditions suggest a target for therapeutic intervention. Research continues to explore how modulating CCR1 activity might impact disease progression and symptom severity in affected individuals.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Chemokine receptor that plays a crucial role in regulating immune cell migration, inflammation, and immune responses (PubMed : 14991608). Contributes to the inflammatory response by recruiting immune cells, such as monocytes, macrophages, T-cells, and dendritic cells, to sites of inflammation for the clearance of pathogens and the resolution of tissue damage. When activated by its ligands including CCL3, CCL5-9, CCL13-16 and CCL23, triggers a signaling cascade within immune cells, leading to their migration towards the source of the chemokine (PubMed : 15905581, PubMed : 38417634). For example, mediates neutrophil migration after activation by CCL3 leading to the sequential release of TNF and leukotriene B4 (By similarity). Also mediates monocyte migration upon CXCL4 binding (PubMed : 29930254). Activation by CCL5 results in neuroinflammation through the ERK1/2 signaling pathway (By similarity).
See full target information CCR1
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Product promise

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