Anti-CCR1 antibody [HL2840] - BSA and Azide free

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-CCR1 antibody [HL2840] - BSA and Azide free (AB325842)

ab325842 detects CCR1 protein by immunofluorescent analysis. Sample : THP-1 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green : CCR1 stained by ab325842 diluted at 1 : 100. Blue : Fluoroshield with DAPI.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CCR1 antibody [HL2840] - BSA and Azide free (AB325842)

ab325842 detects CCR1 protein by immunohistochemical analysis. Sample : Paraffin-embedded human tissues. CCR1 stained by ab325842diluted at 1 : 100. Antigen Retrieval : Citrate buffer, pH 6.0, 15 min. Corresponding RNA expression data for the same tissues are based on NCBI.

• WB

Supplier Data

Western blot - Anti-CCR1 antibody [HL2840] - BSA and Azide free (AB325842)

Whole cell extract (30 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with ab325842 diluted at 1 : 1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

All lanes:

Western blot - Anti-CCR1 antibody [HL2840] - BSA and Azide free (ab325842) at 1/1000 dilution

All lanes:

PG-4 whole cell extract at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

true

• WB

Supplier Data

Western blot - Anti-CCR1 antibody [HL2840] - BSA and Azide free (AB325842)

Non-transfected (–) and transfected (+) boiled and unboiled 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with ab325842 diluted at 1 : 5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

All lanes:

Western blot - Anti-CCR1 antibody [HL2840] - BSA and Azide free (ab325842) at 1/5000 dilution

Lanes 1 and 3:

Non-transfected (–) boiled 293T whole cell extracts at 30 µg

Lane 2:

DDDDK-tagged CCR1 transfected (+) boiled 293T whole cell extracts at 30 µg

Lane 4:

DDDDK-tagged CCR1 transfected (+) unboiled 293T whole cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

false