Anti-CCR2 antibody [EPR20844]

• Flow Cyt

Supplier Data

Flow Cytometry - Anti-CCR2 antibody [EPR20844] (AB216863)

Flow cytometric analysis of HEK-293T (human embryonic kidney epithelial cell) transfected with GFP-tagged CCR2 expression vector, cell line labelling CCR2 with ab216863 at 1/600 dilution (Right) and an isotype control Rabbit monoclonal IgG (ab172730) (Left). Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) at 1/2000 was used as the secondary antibody. Gated on viable cells. Only GFP(+) population give a positive signal.

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-CCR2 antibody [EPR20844] (AB216863)

Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HEK-293T (human embryonic kidney epithelial cell) cells labelling CCR2 with ab216863 at 1/100 dilution, followed by Alexa Fluor^® 647 Goat anti-Rabbit secondary (ab150079) secondary antibody at 1/1000 dilution (green). Confocal image showing membranous staining in 293T cells transfected with GFP-tagged CCR2 expression vector. DAPI was used as the nuclear countertsain (Blue).

Secondary antibody only control :
Control 1 : Alexa Fluor^® 647 Goat anti-Rabbit secondary (ab150079) secondary antibody in GFP transfected HEK-293T cells.
Control 2 : Alexa Fluor^® 647 Goat anti-Rabbit secondary (ab150079) secondary antibody in CCR2-GFP transfected cells.

• Flow Cyt

Lab

Flow Cytometry - Anti-CCR2 antibody [EPR20844] (AB216863)

Flow cytometry staining of C57 BL/6 mouse splenocytes with ab216863 (right) or Rabbit monoclonal IgG (ab172730) (left). Cells were incubated for 30 min on ice in 1x PBS containing 10μg/ml anti CD16/CD32 and 10% normal goat serum to block FC receptors and non-specific protein-protein interaction followed by the antibody ab216863 or Rabbit monoclonal IgG (ab172730) (1x 10^6 in 100 μl at 1.0 μg/ml (1/644)) for 30min on ice. The cells were simultaneously stained with Ly6G. The secondary antibody Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed was incubated at 1/4000 for 30min on ice. Acquisition of >30000 events were collected using a 50 mW Blue laser (488nm) and 525/40 bandpass filter. Events were gated on viable cells.

• Flow Cyt

Lab

Flow Cytometry - Anti-CCR2 antibody [EPR20844] (AB216863)

Flow cytometry staining of C57 BL/6 mouse splenocytes with ab216863 (right) or Rabbit monoclonal IgG (ab172730) (left). Cells were incubated for 30 min on ice in 1x PBS containing 10μg/ml anti CD16/CD32 and 10% normal goat serum to block FC receptors and non-specific protein-protein interaction followed by the antibody ab216863 or Rabbit monoclonal IgG (ab172730) (1x 10^6 in 100 μl at 1.0 μg/ml (1/644)) for 30min on ice. The cells were simultaneously stained with CD11b.

The secondary antibody Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed was incubated at 1/4000 for 30min on ice.

Acquisition of >30000 events were collected using a 50 mW Blue laser (488nm) and 525/40 bandpass filter. Events were gated on viable cells.

• Flow Cyt

Lab

Flow Cytometry - Anti-CCR2 antibody [EPR20844] (AB216863)

Flow cytometry staining of C57 BL/6 mouse bone marrow cells with ab216863 (right) or Rabbit monoclonal IgG (ab172730) (left). Cells were incubated for 30 min on ice in 1x PBS containing 10μg/ml anti CD16/CD32 and 10% normal goat serum to block FC receptors and non-specific protein-protein interaction followed by the antibody ab216863 or Rabbit monoclonal IgG (ab172730) (1x 10^6 in 100 μl at 1.0 μg/ml (1/624)) for 30min on ice. The cells were simultaneously stained with Ly6G.

The secondary antibody Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed was incubated at 1/4000 for 30min on ice.

Acquisition of >30000 events were collected using a 50 mW Blue laser (488nm) and 525/40 bandpass filter. Events were gated on viable cells.

• Flow Cyt

Lab

Flow Cytometry - Anti-CCR2 antibody [EPR20844] (AB216863)

Flow cytometry staining of C57 BL/6 mouse bone marrow cells with ab216863 (right) or Recombinant Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (left). Cells were incubated for 30 mins on ice in 1x PBS containing 10μg/ml anti CD16/CD32 and 10% normal goat serum to block FC receptors and non-specific protein-protein interaction followed by the antibody ab216863 or Recombinant Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (1x 10⁶ in 100 µl at 0.2 μg/ml (1/11250 dilution)) for 30 mins on ice. The cells were simultaneously stained with CD11b.

The secondary antibody Goat Anti-Rabbit IgG H&L (Alexa Fluor^® 488) preadsorbed was incubated at 1/4000 dilution for 30 mins on ice

Acquisition of >30000 events were collected using a 50 mW Blue laser (488nm) and 525/40 bandpass filter. Events were gated on live cells.