Rabbit Recombinant Monoclonal CCR2 antibody. Carrier free. Suitable for Flow Cyt, I-ELISA and reacts with Human, Recombinant fragment - Human samples.
IgG
Rabbit
pH: 7.2 - 7.4
Constituents: PBS
Liquid
Monoclonal
Flow Cyt | I-ELISA | WB | IHC-P | ICC/IF | |
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Human | Tested | Expected | Not recommended | Not recommended | Not recommended |
Recombinant fragment - Human | Not recommended | Tested | Not recommended | Not recommended | Not recommended |
Species | Dilution info | Notes |
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Species Human | Dilution info - | Notes - |
Species | Dilution info | Notes |
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Species Recombinant fragment - Human | Dilution info - | Notes - |
Species | Dilution info | Notes |
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Species Recombinant fragment - Human | Dilution info - | Notes - |
Species | Dilution info | Notes |
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Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
Species | Dilution info | Notes |
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Species Human, Recombinant fragment - Human | Dilution info - | Notes - |
Species | Dilution info | Notes |
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Species Human, Recombinant fragment - Human | Dilution info - | Notes - |
Species | Dilution info | Notes |
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Species Human, Recombinant fragment - Human | Dilution info - | Notes - |
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Key functional receptor for CCL2 but can also bind CCL7, and CCL12 (PubMed:23408426, PubMed:38157855, PubMed:8048929, PubMed:8146186). Also transduces signaling mediated by CCL13 (PubMed:38157855). Its binding with CCL2 on monocytes and macrophages mediates chemotaxis and migration induction through the activation of the PI3K cascade, the small G protein Rac and lamellipodium protrusion (PubMed:38157855). Also acts as a receptor for the beta-defensin DEFB106A/DEFB106B (PubMed:23938203). Regulates the expression of T-cell inflammatory cytokines and T-cell differentiation, promoting the differentiation of T-cells into T-helper 17 cells (Th17) during inflammation (By similarity). Facilitates the export of mature thymocytes by enhancing directional movement of thymocytes to sphingosine-1-phosphate stimulation and up-regulation of S1P1R expression; signals through the JAK-STAT pathway to regulate FOXO1 activity leading to an increased expression of S1P1R (By similarity). Plays an important role in mediating peripheral nerve injury-induced neuropathic pain (By similarity). Increases NMDA-mediated synaptic transmission in both dopamine D1 and D2 receptor-containing neurons, which may be caused by MAPK/ERK-dependent phosphorylation of GRIN2B/NMDAR2B (By similarity). Mediates the recruitment of macrophages and monocytes to the injury site following brain injury (By similarity).(Microbial infection) Alternative coreceptor with CD4 for HIV-1 infection.
CMKBR2, CCR2, CMKBR2, C-C chemokine receptor type 2, C-C CKR-2, CC-CKR-2, CCR-2, CCR2, Monocyte chemoattractant protein 1 receptor, MCP-1-R
Rabbit Recombinant Monoclonal CCR2 antibody. Carrier free. Suitable for Flow Cyt, I-ELISA and reacts with Human, Recombinant fragment - Human samples.
IgG
Rabbit
pH: 7.2 - 7.4
Constituents: PBS
Liquid
Monoclonal
Yes
EPR22191
Affinity purification Protein A
Blue Ice
+4°C
+4°C
Do Not Freeze
ab243292 is the carrier-free version of Anti-CCR2 antibody [EPR22191] ab227236.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This supplementary information is collated from multiple sources and compiled automatically.
CCR2 also known as C-C chemokine receptor type 2 is a protein involved in immune cell trafficking. It is a member of the G-protein-coupled receptor family and has a molecular mass of approximately 43 kDa. CCR2 is primarily expressed on monocytes dendritic cells and certain subsets of T cells. It functions as a receptor for monocyte chemoattractant proteins (MCPs) with MCP-1 (CCL2) being the most well-known ligand. The interaction between CCR2 and its ligands directs the movement of these immune cells to sites of inflammation or tissue injury.
CCR2 plays an important role in mediating leukocyte migration. It acts in the immune system to guide monocytes from the bloodstream into tissues contributing to immune surveillance and response. CCR2 operates not as part of a larger receptor complex but it does interact closely with other chemokine receptors which may influence its signaling. The receptor's activity has critical implications for inflammatory processes and lies at the heart of many immune responses.
CCR2 is integrally involved in the chemokine signaling pathway and the inflammatory response pathway. Its function in these pathways highlights its role in modulating immune cell infiltration during immune challenges. The receptor also interfaces with other important signaling proteins such as CCR5 which like CCR2 is another chemokine receptor involved in mediating immune cell movement. These interactions overlap and complement each other offering nuanced regulation of immune cell dynamics.
CCR2 has connections to conditions such as atherosclerosis and rheumatoid arthritis. In atherosclerosis the receptor's involvement in monocyte recruitment to the arterial wall is an important step in plaque formation. Its role in rheumatoid arthritis centers on the promotion of leukocyte infiltration into the joint tissues contributing to inflammation and joint damage. CCR2's connection to such disorders often aligns with a similar role played by other chemokine receptors like CCR5 highlighting its relevance in inflammation-related pathologies.
We have tested this species and application combination and it works. It is covered by our product promise.
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
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Flow cytometric analysis of human peripheral blood mononuclear cell (PBMC) labeling CCR2 with Anti-CCR2 antibody [EPR22191] ab227236 at 1/500 dilution (right panel) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (left panel). Goat Anti-Rabbit IgG H&L (Dylight® 488) (ab98462) at 1/2000 dilution was used as the secondary antibody.
Cells were stained with Alexa Fluor® 647-conjugated CD14 and rabbit IgG (Left) or CCR2 (Right). Data shown were gated on viable cells (PMID:10811011; PMID:22403612).
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol and sodium azide (Anti-CCR2 antibody [EPR22191] ab227236).
This data was developed using Anti-CCR2 antibody [EPR22191] ab227236, the same antibody clone in a different buffer formulation.ELISA analysis of Human CCR2 Protein recombinant protein at 1000 ng/ml with Anti-CCR2 antibody [EPR22191] ab227236. An Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) at 1/2500 dilution was used as the secondary antibody.
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