Rabbit Recombinant Monoclonal CCR3 antibody. Carrier free. Suitable for Dot, ICC/IF, Flow Cyt and reacts with Synthetic peptide - Mouse, Mouse samples.
Images
![Flow Cytometry - Anti-CCR3 antibody [EPR27419-30] - BSA and Azide free (AB318267), expandable thumbnail](https://content.abcam.com/products/images/ccr3-antibody-epr27419-30-bsa-and-azide-free-ab318267--flow-cytometry-img433122.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry - Anti-CCR3 antibody [EPR27419-30] - BSA and Azide free (AB318267)")
![Flow Cytometry - Anti-CCR3 antibody [EPR27419-30] - BSA and Azide free (AB318267), expandable thumbnail](https://content.abcam.com/products/images/ccr3-antibody-epr27419-30-bsa-and-azide-free-ab318267--flow-cytometry-img433121.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry - Anti-CCR3 antibody [EPR27419-30] - BSA and Azide free (AB318267)")
![Flow Cytometry - Anti-CCR3 antibody [EPR27419-30] - BSA and Azide free (AB318267), expandable thumbnail](https://content.abcam.com/products/images/ccr3-antibody-epr27419-30-bsa-and-azide-free-ab318267--flow-cytometry-img433120.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry - Anti-CCR3 antibody [EPR27419-30] - BSA and Azide free (AB318267)")
![Immunocytochemistry/ Immunofluorescence - Anti-CCR3 antibody [EPR27419-30] - BSA and Azide free (AB318267), expandable thumbnail](https://content.abcam.com/products/images/ccr3-antibody-epr27419-30-bsa-and-azide-free-ab318267--immunocytochemistry-immunofluorescence-img433063.jpg/_jcr_content/renditions/webp-475.webp "Immunocytochemistry/ Immunofluorescence - Anti-CCR3 antibody [EPR27419-30] - BSA and Azide free (AB318267)")
![Dot Blot - Anti-CCR3 antibody [EPR27419-30] - BSA and Azide free (AB318267), expandable thumbnail](https://content.abcam.com/products/images/ccr3-antibody-epr27419-30-bsa-and-azide-free-ab318267--dot-blot-img433140.jpg/_jcr_content/renditions/webp-475.webp "Dot Blot - Anti-CCR3 antibody [EPR27419-30] - BSA and Azide free (AB318267)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| Dot | ICC/IF | Flow Cyt | WB | |
|---|---|---|---|---|
| Human | Not recommended | Not recommended | Not recommended | Not recommended |
| Mouse | Expected | Tested | Tested | Not recommended |
| Rat | Not recommended | Not recommended | Not recommended | Not recommended |
| Synthetic peptide - Mouse | Tested | Not recommended | Not recommended | Not recommended |
Dot
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Synthetic peptide - Mouse | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info Use at an assay dependent concentration. | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Rat | Dilution info - | Notes - |
ICC/IF
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Synthetic peptide - Mouse, Human, Rat | Dilution info - | Notes - |
Flow Cyt
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Synthetic peptide - Mouse, Human, Rat | Dilution info - | Notes - |
WB
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Rat, Mouse, Synthetic peptide - Mouse | Dilution info - | Notes - |
Target data
Function
Receptor for C-C type chemokine. Binds and responds to a variety of chemokines, including CCL11, CCL26, CCL7, CCL13, RANTES(CCL5) and CCL15. Subsequently transduces a signal by increasing the intracellular calcium ions level. In addition acts as a possible functional receptor for NARS1.
Alternative names
CD193, Cmkbr1l2, Cmkbr3, Ccr3, Probable C-C chemokine receptor type 3, C-C CKR-3, CC-CKR-3, CCR-3, CCR3, CKR3, Macrophage inflammatory protein 1-alpha receptor-like 2, MIP-1 alpha RL2
Recommended products
Rabbit Recombinant Monoclonal CCR3 antibody. Carrier free. Suitable for Dot, ICC/IF, Flow Cyt and reacts with Synthetic peptide - Mouse, Mouse samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: 100% PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR27419-30
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
Notes
ab318267 is the carrier-free version of Anti-CCR3 antibody [EPR27419-30] ab318266.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
CCR3 also known as C-C chemokine receptor type 3 is a protein that acts as a receptor for chemokines. The molecular weight of CCR3 is approximately 43 kDa. This protein is predominantly expressed in eosinophils and basophils and is also found in specific subsets of T cells. The expression of CCR3 in these cells plays an important role in directing their migration and activity. This receptor is part of the G protein-coupled receptor family which transduce signals across the cell membrane.
- Biological function summary
CCR3 facilitates chemokine-directed migration of white blood cells an essential function for immune responses. It binds to several chemokines like eotaxin eotaxin-2 and RANTES which mediates the recruitment of eosinophils and other cells to sites of inflammation. CCR3 does not form part of a protein complex instead it interacts directly with its chemokine ligands. This activity enhances the body's ability to respond to allergic reactions and inflammatory processes.
- Pathways
CCR3 plays a role in the signaling pathways that orchestrate immune cell trafficking and activation. It is involved in the chemokine signaling pathway which regulates cell movement and positioning and the calcium mobilization pathway through its ligand interaction. CCR3 along with other receptors like CCR5 and CXCR4 facilitates the communication between immune cells aiding in directed cell movement towards the inflammation site.
- Associated diseases and disorders
CCR3 is notably involved in allergic disorders and asthma. Its expression influences the recruitment of eosinophils which are key players in these conditions. This receptor is linked to heightened inflammatory responses leading to tissue damage in chronic allergic diseases. The interaction of CCR3 with chemokines like eotaxin is significant in the pathophysiology of these diseases making it a potential target for therapeutic intervention to mitigate symptoms of chronic allergic responses.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
5 product images
![Flow Cytometry - Anti-CCR3 antibody [EPR27419-30] - BSA and Azide free (ab318267), expandable thumbnail](https://content.abcam.com/products/images/ccr3-antibody-epr27419-30-bsa-and-azide-free-ab318267--flow-cytometry-img433122.jpg "Flow Cytometry - Anti-CCR3 antibody [EPR27419-30] - BSA and Azide free (ab318267)")
Flow Cytometry - Anti-CCR3 antibody [EPR27419-30] - BSA and Azide free (ab318267)
This data was developed using Anti-CCR3 antibody [EPR27419-30] ab318266, the same antibody clone in a different buffer formulation.
Flow cytometric analysis of Mouse bone marrow cells labelling CCR3 with Anti-CCR3 antibody [EPR27419-30] ab318266 at 1/50 dilution (1ug) / Right compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).
Goat anti-Rabbit IgG (Alexa Fluor® 647, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 647) preadsorbed ab150083) at 1/5000 dilution was used as the secondary antibody.
Cells were co-stained with anti-CD11b conjugated to Brilliant Violet 421.
Gated on viable cells.![Flow Cytometry - Anti-CCR3 antibody [EPR27419-30] - BSA and Azide free (ab318267), expandable thumbnail](https://content.abcam.com/products/images/ccr3-antibody-epr27419-30-bsa-and-azide-free-ab318267--flow-cytometry-img433121.jpg "Flow Cytometry - Anti-CCR3 antibody [EPR27419-30] - BSA and Azide free (ab318267)")
Flow Cytometry - Anti-CCR3 antibody [EPR27419-30] - BSA and Azide free (ab318267)
This data was developed using Anti-CCR3 antibody [EPR27419-30] ab318266, the same antibody clone in a different buffer formulation.
Flow cytometric analysis of Mouse bone marrow cells labelling CCR3 with Anti-CCR3 antibody [EPR27419-30] ab318266 at 1/50 dilution (1ug) / Right compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).
Goat anti-Rabbit IgG (Alexa Fluor® 647, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 647) preadsorbed ab150083) at 1/5000 dilution was used as the secondary antibody.
Cells were co-stained with anti-CD3 cojugated to Alexa Fluor®488.
Gated on viable cells.![Flow Cytometry - Anti-CCR3 antibody [EPR27419-30] - BSA and Azide free (ab318267), expandable thumbnail](https://content.abcam.com/products/images/ccr3-antibody-epr27419-30-bsa-and-azide-free-ab318267--flow-cytometry-img433120.jpg "Flow Cytometry - Anti-CCR3 antibody [EPR27419-30] - BSA and Azide free (ab318267)")
Flow Cytometry - Anti-CCR3 antibody [EPR27419-30] - BSA and Azide free (ab318267)
This data was developed using Anti-CCR3 antibody [EPR27419-30] ab318266, the same antibody clone in a different buffer formulation.
Flow cytometric analysis of J774A.1 (mouse reticulum cell sarcoma monocyte/macrophage) (Right) /
A20 (mouse reticulum sarcoma B lymphocyte) (Left) cells labelling CCR3 with Anti-CCR3 antibody [EPR27419-30] ab318266 at 1/50 dilution (1ug) / Magenta compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).Goat anti-Rabbit IgG (Alexa Fluor® 647, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 647) preadsorbed ab150083) at 1/5000 dilution was used as the secondary antibody.
Gated on viable cells.
Low expression: A20.![Immunocytochemistry/ Immunofluorescence - Anti-CCR3 antibody [EPR27419-30] - BSA and Azide free (ab318267), expandable thumbnail](https://content.abcam.com/products/images/ccr3-antibody-epr27419-30-bsa-and-azide-free-ab318267--immunocytochemistry-immunofluorescence-img433063.jpg "Immunocytochemistry/ Immunofluorescence - Anti-CCR3 antibody [EPR27419-30] - BSA and Azide free (ab318267)")
Immunocytochemistry/ Immunofluorescence - Anti-CCR3 antibody [EPR27419-30] - BSA and Azide free (ab318267)
This data was developed using Anti-CCR3 antibody [EPR27419-30] ab318266, the same antibody clone in a different buffer formulation.
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized J774A.1(mouse reticulum cell sarcoma monocyte/macrophage) cells labelling CCR3 with Anti-CCR3 antibody [EPR27419-30] ab318266 at 1/50 (10.1 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2 ug/ml dilution (Green).
Confocal image showing cytoplasmic staining in J774A.1 cell line (shown in green). The counterstain was observed in magenta. Nuclear DNA was labelled with DAPI (shown in blue).
Low expression: A20.
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Magenta). The Nuclear counterstain was DAPI (Blue).
Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/ml dilution.
![Dot Blot - Anti-CCR3 antibody [EPR27419-30] - BSA and Azide free (ab318267), expandable thumbnail](https://content.abcam.com/products/images/ccr3-antibody-epr27419-30-bsa-and-azide-free-ab318267--dot-blot-img433140.jpg "Dot Blot - Anti-CCR3 antibody [EPR27419-30] - BSA and Azide free (ab318267)")
Dot Blot - Anti-CCR3 antibody [EPR27419-30] - BSA and Azide free (ab318267)
This data was developed using Anti-CCR3 antibody [EPR27419-30] ab318266, the same antibody clone in a different buffer formulation.
Dot blot analysis of CCR3 using Anti-CCR3 antibody [EPR27419-30] ab318266 at 1:1000 (0.505 ug/ml) followed by a Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1:100,000 dilution.
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
All lanes: Dot Blot - Anti-CCR3 antibody [EPR27419-30] (Anti-CCR3 antibody [EPR27419-30] ab318266) at 1/1000 dilution
All lanes: Mouse CCR3 peptide
SecondaryAll lanes: Dot Blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Exposure time: 180s
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com