Rabbit Recombinant Monoclonal CCR3 antibody. Carrier free. Suitable for IHC-P and reacts with Human samples. Cited in 12 publications.
Images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CCR3 antibody [Y31] - Low endotoxin, Azide free (AB228341), expandable thumbnail](https://content.abcam.com/products/images/ccr3-antibody-y31-low-endotoxin-azide-free-ab228341--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img58514.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CCR3 antibody [Y31] - Low endotoxin, Azide free (AB228341)")
Publications
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- Frontiers in pharmacology 8:2072017CCR3 Is Associated with the Death of a Photoreceptor Cell-line Induced by Light Exposure.
- The Journal of biological chemistry 291:19532-442016CC-Chemokine Ligand 2 (CCL2) Suppresses High Density Lipoprotein (HDL) Internalization and Cholesterol Efflux via CC-Chemokine Receptor 2 (CCR2) Induction and p42/44 Mitogen-activated Protein Kinase (MAPK) Activation in Human Endothelial Cells.
- Oncotarget 7:36842-368532016Crosstalk between CCL7 and CCR3 promotes metastasis of colon cancer cells via ERK-JNK signaling pathways.Applications:Flow CytReactive species:Unspecified reactive speciesYeo Song Lee,So-Young Kim,Su Jeong Song,Hye Kyung Hong,Yura Lee,Bo Young Oh,Woo Yong Lee,Yong Beom ChoPubMed 27167205
- Nature communications 7:102302016Periprostatic adipocytes act as a driving force for prostate cancer progression in obesity.Applications:Unspecified applicationReactive species:Unspecified reactive speciesVictor Laurent et. al.PubMed 26756352
- Cell reports 10:1173-862015ROCK-isoform-specific polarization of macrophages associated with age-related macular degeneration.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSouska Zandi,Shintaro Nakao,Kwang-Hoon Chun,Paolo Fiorina,Dawei Sun,Ryoichi Arita,Ming Zhao,Enoch Kim,Olivier Schueller,Stewart Campbell,Mahdi Taher,Mark Ivan Melhorn,Alexander Schering,Francesca Gatti,Sara Tezza,Fang Xie,Andrea Vergani,Shigeo Yoshida,Keijiro Ishikawa,Muneo Yamaguchi,Fumiyuki Sasaki,Ruth Schmidt-Ullrich,Yasuaki Hata,Hiroshi Enaida,Mitsuko Yuzawa,Takehiko Yokomizo,Young-Bum Kim,Paul Sweetnam,Tatsuro Ishibashi,Ali Hafezi-MoghadamPubMed 25704819
- Biomedical optics express 5:3521-402014Probing the immune and healing response of murine intestinal mucosa by time-lapse 2-photon microscopy of laser-induced lesions with real-time dosimetry.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRegina Orzekowsky-Schroeder et. al.PubMed 25360369
- Human reproduction (Oxford, England) 28:1497-5072013Control of extravillous trophoblast function by the eotaxins CCL11, CCL24 and CCL26.
- Journal of allergy 2012:8191762012Immunolocalization of NLRP3 Inflammasome in Normal Murine Airway Epithelium and Changes following Induction of Ovalbumin-Induced Airway Inflammation.Applications:IHC-PReactive species:MouseHai B Tran,Martin D Lewis,Lor Wai Tan,Susan E Lester,Leonie M Baker,Jia Ng,Monica A Hamilton-Bruce,Catherine L Hill,Simon A Koblar,Maureen Rischmueller,Richard E Ruffin,Peter J Wormald,Peter D Zalewski,Carol J LangPubMed 22523501
- Annals of dermatology 22:412-72010Chemokine Receptor CCR3 Expression in Malignant Cutaneous Tumors.
- American journal of clinical pathology 133:281-902010Expression of master regulators of helper T-cell differentiation in peripheral T-cell lymphoma, not otherwise specified, by immunohistochemical analysis.Applications:Unspecified applicationReactive species:Unspecified reactive speciesYosuke Matsumoto,Shigeo Horiike,Muneo Ohshiro,Mio Yamamoto,Nana Sasaki,Yasuhiko Tsutsumi,Tsutomu Kobayashi,Daisuke Shimizu,Hitoji Uchiyama,Junya Kuroda,Kenichi Nomura,Chihiro Shimazaki,Masafumi TaniwakiPubMed 20093238
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IHC-P | IP | Flow Cyt | WB | ICC/IF | |
|---|---|---|---|---|---|
| Human | Tested | Not recommended | Not recommended | Not recommended | Not recommended |
| Mouse | Predicted | Not recommended | Not recommended | Not recommended | Not recommended |
| Rat | Predicted | Not recommended | Not recommended | Not recommended | Not recommended |
| Macaque monkey | Predicted | Not recommended | Not recommended | Not recommended | Not recommended |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval before commencing with IHC staining protocol. |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat, Macaque monkey | Dilution info - | Notes - |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat, Macaque monkey | Dilution info - | Notes - |
Flow Cyt
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat, Macaque monkey | Dilution info - | Notes - |
WB
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat, Macaque monkey | Dilution info - | Notes - |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat, Macaque monkey | Dilution info - | Notes - |
Associated Products
Select an associated product type
6 products for Alternative Product
1 product for Alternative Version
Target data
Function
Receptor for C-C type chemokine. Binds and responds to a variety of chemokines, including CCL11, CCL26, CCL7, CCL13, RANTES(CCL5) and CCL15 (PubMed:7622448, PubMed:8642344, PubMed:8676064). Subsequently transduces a signal by increasing the intracellular calcium ions level (PubMed:8676064). In addition acts as a possible functional receptor for NARS1 (PubMed:30171954). (Microbial infection) Alternative coreceptor with CD4 for HIV-1 infection.
Alternative names
CD193, CMKBR3, CCR3, C-C chemokine receptor type 3, C C CKR3, C-C CKR-3, CC-CKR-3, CCR-3, CKR 3, CKR3, Eosinophil eotaxin receptor
Recommended products
Rabbit Recombinant Monoclonal CCR3 antibody. Carrier free. Suitable for IHC-P and reacts with Human samples. Cited in 12 publications.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- Y31
- Purification technique
- Affinity purification Protein A
- Specificity
The mouse and rat recommendation is for WB use only. We do not guarantee IHC-P for mouse and rat.
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab228341 is the carrier-free version of Anti-CCR3 antibody [Y31] ab32512.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Our Low endotoxin, azide-free formats have low endotoxin level (1 EU/mg, determined by the TAL assay) and are free from azide, to achieve consistent experimental results in functional assays.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
CCR3 also known as C-C chemokine receptor type 3 is a protein that acts as a receptor for chemokines. The molecular weight of CCR3 is approximately 43 kDa. This protein is predominantly expressed in eosinophils and basophils and is also found in specific subsets of T cells. The expression of CCR3 in these cells plays an important role in directing their migration and activity. This receptor is part of the G protein-coupled receptor family which transduce signals across the cell membrane.
- Biological function summary
CCR3 facilitates chemokine-directed migration of white blood cells an essential function for immune responses. It binds to several chemokines like eotaxin eotaxin-2 and RANTES which mediates the recruitment of eosinophils and other cells to sites of inflammation. CCR3 does not form part of a protein complex instead it interacts directly with its chemokine ligands. This activity enhances the body's ability to respond to allergic reactions and inflammatory processes.
- Pathways
CCR3 plays a role in the signaling pathways that orchestrate immune cell trafficking and activation. It is involved in the chemokine signaling pathway which regulates cell movement and positioning and the calcium mobilization pathway through its ligand interaction. CCR3 along with other receptors like CCR5 and CXCR4 facilitates the communication between immune cells aiding in directed cell movement towards the inflammation site.
- Associated diseases and disorders
CCR3 is notably involved in allergic disorders and asthma. Its expression influences the recruitment of eosinophils which are key players in these conditions. This receptor is linked to heightened inflammatory responses leading to tissue damage in chronic allergic diseases. The interaction of CCR3 with chemokines like eotaxin is significant in the pathophysiology of these diseases making it a potential target for therapeutic intervention to mitigate symptoms of chronic allergic responses.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
1 product image
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CCR3 antibody [Y31] - Low endotoxin, Azide free (ab228341), expandable thumbnail](https://content.abcam.com/products/images/ccr3-antibody-y31-low-endotoxin-azide-free-ab228341--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img58514.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CCR3 antibody [Y31] - Low endotoxin, Azide free (ab228341)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CCR3 antibody [Y31] - Low endotoxin, Azide free (ab228341)
This IHC data was generated using the same anti-CCR3 antibody clone, Y31, in a different buffer formulation (cat# Anti-VEGF Receptor 1 antibody [Y103] ab32152).
Immunohistochemical analysis of paraffin-embedded human liver tissue using Anti-CCR3 antibody [Y31] ab32512 at 1/100 dilution.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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