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AB275400

Anti-CCR4 antibody [EPR23502-85] - BSA and Azide free

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Rabbit Recombinant Monoclonal CCR4 antibody. Carrier free. Suitable for Flow Cyt and reacts with Human, Transfected cell line - Human samples.

View Alternative Names

CD194, CMKBR4, CCR4, C-C chemokine receptor type 4, C-C CKR-4, CC-CKR-4, CCR-4, K5-5

4 Images
Flow Cytometry - Anti-CCR4 antibody [EPR23502-85] - BSA and Azide free (AB275400)
  • Flow Cyt

Supplier Data

Flow Cytometry - Anti-CCR4 antibody [EPR23502-85] - BSA and Azide free (AB275400)

This data was developed using ab254376, the same antibody clone in a different buffer formulation.

Flow cytometric analysis of human peripheral blood mononuclear cell (PBMC) cells labeling CCR4 with ab254376 at 1/500 dilution (Right) compared with a Rabbit monoclonal IgG (ab172730) isotype control (Left).

Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody.

Gated on viable lymphocytes

Flow Cytometry - Anti-CCR4 antibody [EPR23502-85] - BSA and Azide free (AB275400)
  • Flow Cyt

Supplier Data

Flow Cytometry - Anti-CCR4 antibody [EPR23502-85] - BSA and Azide free (AB275400)

This data was developed using ab254376, the same antibody clone in a different buffer formulation.

Flow cytometric analysis of HEK-293 (human embryonic kidney epithelial cell, Left) / HDLM-2 (human Hodgkin lymphoma, Right) cells labeling CCR4 with ab254376 at 1/500 dilution (Red) compared with a Rabbit monoclonal IgG (ab172730) isotype control (Black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

Goat anti rabbit IgG (Alexa Fluor® 647, ab150079) at 1/2000 dilution was used as the secondary antibody.

Negative control : HEK-293 (PMID : 22973452).

Gated on viable cells.

Flow Cytometry - Anti-CCR4 antibody [EPR23502-85] - BSA and Azide free (AB275400)
  • Flow Cyt

Supplier Data

Flow Cytometry - Anti-CCR4 antibody [EPR23502-85] - BSA and Azide free (AB275400)

This data was developed using ab254376, the same antibody clone in a different buffer formulation.

Flow cytometric analysis of HEK-293T (human embryonic kidney epithelial cell) (transfected with GFP tagged CCR4 overexpression vector) cells labeling CCR4 with ab254376 at 1/500 dilution (Right) compared with a Rabbit monoclonal IgG (ab172730) isotype control (Left).

Goat anti rabbit IgG (Alexa Fluor® 647, ab150079) at 1/2000 dilution was used as the secondary antibody.

Gated on viable cells.

Flow Cytometry - Anti-CCR4 antibody [EPR23502-85] - BSA and Azide free (AB275400)
  • Flow Cyt

Lab

Flow Cytometry - Anti-CCR4 antibody [EPR23502-85] - BSA and Azide free (AB275400)

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab254376).

Flow cytometry staining of human peripheral blood mononuclear cells (PBMCs) with ab254376 (right) or Recombinant Rabbit IgG, monoclonal [EPR25A] - Isotype Control (left). PBMCs were incubated for 30 mins on ice in 1x PBS containing 10 µg/ml human IgG and 10 % normal goat serum to block FC receptors and non-specific protein-protein interaction followed by the antibody ab254376 or Recombinant Rabbit IgG, monoclonal [EPR25A] - Isotype Control (1x 106 in 100 µl at 5.0 μg/ml (1/2,300 dilution)) for 30 mins on ice. The cells were simultaneously stained with CD3.

The secondary antibody Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed was incubated at 1/4000 dilution for 30 mins on ice

Acquisition of >30000 events were collected using a 50 mW Blue laser (488nm) and 525/40 bandpass filter. Events were gated on CD3+.

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR23502-85

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

Flow Cyt

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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Product details

ab275400 is the carrier-free version of ab254376.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CCR4 also known as C-C chemokine receptor type 4 is a G protein-coupled receptor with the molecular mass of approximately 41 kDa. It is predominantly expressed on the surface of T-helper 2 cells regulatory T cells and certain dendritic cells. CCR4 functions by binding chemokines such as CCL17 and CCL22 facilitating chemotaxis of immune cells to sites of inflammation. Researchers often refer to antibodies targeting CCR4 like mogamulizumab when studying immune responses.
Biological function summary

CCR4 plays an important role in the trafficking of immune cells to inflammatory sites therefore regulating immune response and tolerance. It is part of the chemokine receptor family which is integral to the immune system's function. CCR4 helps maintain the balance between effector and regulatory T cells contributing to immunological homeostasis. Its involvement in migration and positioning of immune cells within lymphoid and peripheral tissues highlights its importance in maintaining immune surveillance.

Pathways

CCR4 significantly influences the chemokine signaling pathway which is pivotal in directing the movement and positioning of immune cells. This receptor interacts with key proteins like CCL17 and CCL22 to mediate cell chemotaxis in these pathways. Additionally CCR4 is associated with the T-cell receptor signaling pathway where it coordinates with other receptors and proteins to modulate immune responses.

Increased CCR4 expression has connections with various conditions such as adult T-cell leukemia/lymphoma and cutaneous T-cell lymphomas. In these diseases CCR4 assists tumor cells in evading the immune system fueling disease progression. Proteins like TARC (thymus and activation-regulated chemokine) and MDC (macrophage-derived chemokine) ligands for CCR4 often facilitate tumor cell migration and proliferation. Therapeutic approaches targeting CCR4 such as the anti-CCR4 antibody mogamulizumab have shown potential in treating these malignancies by disrupting these interactions.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

High affinity receptor for the C-C type chemokines CCL17/TARC, CCL22/MDC and CKLF isoform 1/CKLF1. The activity of this receptor is mediated by G(i) proteins which activate a phosphatidylinositol-calcium second messenger system. Can function as a chemoattractant homing receptor on circulating memory lymphocytes and as a coreceptor for some primary HIV-2 isolates. In the CNS, could mediate hippocampal-neuron survival.
See full target information CCR4
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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