Rabbit Recombinant Monoclonal CCR4 antibody. Carrier free. Suitable for Flow Cyt and reacts with Human, Transfected cell line - Human samples.
Images
![Flow Cytometry - Anti-CCR4 antibody [EPR23502-85] - BSA and Azide free (AB275400), expandable thumbnail](https://content.abcam.com/products/images/ccr4-antibody-epr23502-85-bsa-and-azide-free-ab275400--flow-cytometry-img37376.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry - Anti-CCR4 antibody [EPR23502-85] - BSA and Azide free (AB275400)")
![Flow Cytometry - Anti-CCR4 antibody [EPR23502-85] - BSA and Azide free (AB275400), expandable thumbnail](https://content.abcam.com/products/images/ccr4-antibody-epr23502-85-bsa-and-azide-free-ab275400--flow-cytometry-img13068.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry - Anti-CCR4 antibody [EPR23502-85] - BSA and Azide free (AB275400)")
![Flow Cytometry - Anti-CCR4 antibody [EPR23502-85] - BSA and Azide free (AB275400), expandable thumbnail](https://content.abcam.com/products/images/ccr4-antibody-epr23502-85-bsa-and-azide-free-ab275400--flow-cytometry-img13376.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry - Anti-CCR4 antibody [EPR23502-85] - BSA and Azide free (AB275400)")
![Flow Cytometry - Anti-CCR4 antibody [EPR23502-85] - BSA and Azide free (AB275400), expandable thumbnail](https://content.abcam.com/products/images/ccr4-antibody-epr23502-85-bsa-and-azide-free-ab275400--flow-cytometry-img439604.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry - Anti-CCR4 antibody [EPR23502-85] - BSA and Azide free (AB275400)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Constituents: 100% PBS
- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| ICC/IF | IP | Flow Cyt | WB | IHC-P | |
|---|---|---|---|---|---|
| Human | Not recommended | Not recommended | Tested | Not recommended | Not recommended |
| Mouse | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
| Rat | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
| Transfected cell line - Human | Not recommended | Not recommended | Tested | Not recommended | Not recommended |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Transfected cell line - Human, Mouse, Rat | Dilution info - | Notes - |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Transfected cell line - Human, Mouse, Rat | Dilution info - | Notes - |
Flow Cyt
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Transfected cell line - Human | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
WB
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat, Transfected cell line - Human | Dilution info - | Notes - |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat, Transfected cell line - Human | Dilution info - | Notes - |
Associated Products
Select an associated product type
1 product for Alternative Version
Target data
Function
High affinity receptor for the C-C type chemokines CCL17/TARC, CCL22/MDC and CKLF isoform 1/CKLF1. The activity of this receptor is mediated by G(i) proteins which activate a phosphatidylinositol-calcium second messenger system. Can function as a chemoattractant homing receptor on circulating memory lymphocytes and as a coreceptor for some primary HIV-2 isolates. In the CNS, could mediate hippocampal-neuron survival.
Alternative names
CD194, CMKBR4, CCR4, C-C chemokine receptor type 4, C-C CKR-4, CC-CKR-4, CCR-4, K5-5
Recommended products
Rabbit Recombinant Monoclonal CCR4 antibody. Carrier free. Suitable for Flow Cyt and reacts with Human, Transfected cell line - Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
Constituents: 100% PBS
- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR23502-85
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
Notes
ab275400 is the carrier-free version of Anti-CCR4 antibody [EPR23502-85] ab254376.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
CCR4 also known as C-C chemokine receptor type 4 is a G protein-coupled receptor with the molecular mass of approximately 41 kDa. It is predominantly expressed on the surface of T-helper 2 cells regulatory T cells and certain dendritic cells. CCR4 functions by binding chemokines such as CCL17 and CCL22 facilitating chemotaxis of immune cells to sites of inflammation. Researchers often refer to antibodies targeting CCR4 like mogamulizumab when studying immune responses.
- Biological function summary
CCR4 plays an important role in the trafficking of immune cells to inflammatory sites therefore regulating immune response and tolerance. It is part of the chemokine receptor family which is integral to the immune system's function. CCR4 helps maintain the balance between effector and regulatory T cells contributing to immunological homeostasis. Its involvement in migration and positioning of immune cells within lymphoid and peripheral tissues highlights its importance in maintaining immune surveillance.
- Pathways
CCR4 significantly influences the chemokine signaling pathway which is pivotal in directing the movement and positioning of immune cells. This receptor interacts with key proteins like CCL17 and CCL22 to mediate cell chemotaxis in these pathways. Additionally CCR4 is associated with the T-cell receptor signaling pathway where it coordinates with other receptors and proteins to modulate immune responses.
- Associated diseases and disorders
Increased CCR4 expression has connections with various conditions such as adult T-cell leukemia/lymphoma and cutaneous T-cell lymphomas. In these diseases CCR4 assists tumor cells in evading the immune system fueling disease progression. Proteins like TARC (thymus and activation-regulated chemokine) and MDC (macrophage-derived chemokine) ligands for CCR4 often facilitate tumor cell migration and proliferation. Therapeutic approaches targeting CCR4 such as the anti-CCR4 antibody mogamulizumab have shown potential in treating these malignancies by disrupting these interactions.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
4 product images
![Flow Cytometry - Anti-CCR4 antibody [EPR23502-85] - BSA and Azide free (ab275400), expandable thumbnail](https://content.abcam.com/products/images/ccr4-antibody-epr23502-85-bsa-and-azide-free-ab275400--flow-cytometry-img37376.jpg "Flow Cytometry - Anti-CCR4 antibody [EPR23502-85] - BSA and Azide free (ab275400)")
Flow Cytometry - Anti-CCR4 antibody [EPR23502-85] - BSA and Azide free (ab275400)
This data was developed using Anti-CCR4 antibody [EPR23502-85] ab254376, the same antibody clone in a different buffer formulation.
Flow cytometric analysis of human peripheral blood mononuclear cell (PBMC) cells labeling CCR4 with Anti-CCR4 antibody [EPR23502-85] ab254376 at 1/500 dilution (Right) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) isotype control (Left).
Goat anti rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) ab150077) at 1/2000 dilution was used as the secondary antibody.
Gated on viable lymphocytes
![Flow Cytometry - Anti-CCR4 antibody [EPR23502-85] - BSA and Azide free (ab275400), expandable thumbnail](https://content.abcam.com/products/images/ccr4-antibody-epr23502-85-bsa-and-azide-free-ab275400--flow-cytometry-img13068.jpg "Flow Cytometry - Anti-CCR4 antibody [EPR23502-85] - BSA and Azide free (ab275400)")
Flow Cytometry - Anti-CCR4 antibody [EPR23502-85] - BSA and Azide free (ab275400)
This data was developed using Anti-CCR4 antibody [EPR23502-85] ab254376, the same antibody clone in a different buffer formulation.
Flow cytometric analysis of HEK-293T (human embryonic kidney epithelial cell) (transfected with GFP tagged CCR4 overexpression vector) cells labeling CCR4 with Anti-CCR4 antibody [EPR23502-85] ab254376 at 1/500 dilution (Right) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) isotype control (Left).
Goat anti rabbit IgG (Alexa Fluor® 647, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 647) ab150079) at 1/2000 dilution was used as the secondary antibody.
Gated on viable cells.
![Flow Cytometry - Anti-CCR4 antibody [EPR23502-85] - BSA and Azide free (ab275400), expandable thumbnail](https://content.abcam.com/products/images/ccr4-antibody-epr23502-85-bsa-and-azide-free-ab275400--flow-cytometry-img13376.jpg "Flow Cytometry - Anti-CCR4 antibody [EPR23502-85] - BSA and Azide free (ab275400)")
Flow Cytometry - Anti-CCR4 antibody [EPR23502-85] - BSA and Azide free (ab275400)
This data was developed using Anti-CCR4 antibody [EPR23502-85] ab254376, the same antibody clone in a different buffer formulation.
Flow cytometric analysis of HEK-293 (human embryonic kidney epithelial cell, Left) / HDLM-2 (human Hodgkin lymphoma, Right) cells labeling CCR4 with Anti-CCR4 antibody [EPR23502-85] ab254376 at 1/500 dilution (Red) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) isotype control (Black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).
Goat anti rabbit IgG (Alexa Fluor® 647, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 647) ab150079) at 1/2000 dilution was used as the secondary antibody.
Negative control: HEK-293 (PMID: 22973452).
Gated on viable cells.
![Flow Cytometry - Anti-CCR4 antibody [EPR23502-85] - BSA and Azide free (ab275400), expandable thumbnail](https://content.abcam.com/products/images/ccr4-antibody-epr23502-85-bsa-and-azide-free-ab275400--flow-cytometry-img439604.jpg "Flow Cytometry - Anti-CCR4 antibody [EPR23502-85] - BSA and Azide free (ab275400)")
Flow Cytometry - Anti-CCR4 antibody [EPR23502-85] - BSA and Azide free (ab275400)
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-CCR4 antibody [EPR23502-85] ab254376).
Flow cytometry staining of human peripheral blood mononuclear cells (PBMCs) with Anti-CCR4 antibody [EPR23502-85] ab254376 (right) or Recombinant Rabbit IgG, monoclonal [EPR25A] - Isotype Control (left). PBMCs were incubated for 30 mins on ice in 1x PBS containing 10 µg/ml human IgG and 10 % normal goat serum to block FC receptors and non-specific protein-protein interaction followed by the antibody Anti-CCR4 antibody [EPR23502-85] ab254376 or Recombinant Rabbit IgG, monoclonal [EPR25A] - Isotype Control (1x 106 in 100 µl at 5.0 μg/ml (1/2,300 dilution)) for 30 mins on ice. The cells were simultaneously stained with CD3.
The secondary antibody Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed was incubated at 1/4000 dilution for 30 mins on ice
Acquisition of >30000 events were collected using a 50 mW Blue laser (488nm) and 525/40 bandpass filter. Events were gated on CD3+.
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