Rabbit Recombinant Monoclonal CCR4 antibody. Suitable for WB, Flow Cyt and reacts with Human samples.
Images
![Western blot - Anti-CCR4 antibody [EPR26522-156] (AB315446), expandable thumbnail](https://content.abcam.com/products/images/ccr4-antibody-epr26522-156-ab315446--western-blot-img425121.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-CCR4 antibody [EPR26522-156] (AB315446)")
![Western blot - Anti-CCR4 antibody [EPR26522-156] (AB315446), expandable thumbnail](https://content.abcam.com/products/images/ccr4-antibody-epr26522-156-ab315446--western-blot-img425120.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-CCR4 antibody [EPR26522-156] (AB315446)")
![Flow Cytometry - Anti-CCR4 antibody [EPR26522-156] (AB315446), expandable thumbnail](https://content.abcam.com/products/images/ccr4-antibody-epr26522-156-ab315446--flow-cytometry-img425243.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry - Anti-CCR4 antibody [EPR26522-156] (AB315446)")
![Flow Cytometry - Anti-CCR4 antibody [EPR26522-156] (AB315446), expandable thumbnail](https://content.abcam.com/products/images/ccr4-antibody-epr26522-156-ab315446--flow-cytometry-img425242.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry - Anti-CCR4 antibody [EPR26522-156] (AB315446)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| WB | IP | IHC-P | ICC/IF | Flow Cyt | |
|---|---|---|---|---|---|
| Human | Tested | Not recommended | Not recommended | Not recommended | Tested |
| Mouse | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
| Rat | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/1000 | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
IHC-P
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
Flow Cyt
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info 1/50 | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse, Rat | Dilution info - | Notes - |
Associated Products
Select an associated product type
1 product for Alternative Version
Target data
Function
High affinity receptor for the C-C type chemokines CCL17/TARC, CCL22/MDC and CKLF isoform 1/CKLF1. The activity of this receptor is mediated by G(i) proteins which activate a phosphatidylinositol-calcium second messenger system. Can function as a chemoattractant homing receptor on circulating memory lymphocytes and as a coreceptor for some primary HIV-2 isolates. In the CNS, could mediate hippocampal-neuron survival.
Alternative names
CD194, CMKBR4, CCR4, C-C chemokine receptor type 4, C-C CKR-4, CC-CKR-4, CCR-4, K5-5
Recommended products
Rabbit Recombinant Monoclonal CCR4 antibody. Suitable for WB, Flow Cyt and reacts with Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Clone number
- EPR26522-156
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
CCR4 also known as C-C chemokine receptor type 4 is a G protein-coupled receptor with the molecular mass of approximately 41 kDa. It is predominantly expressed on the surface of T-helper 2 cells regulatory T cells and certain dendritic cells. CCR4 functions by binding chemokines such as CCL17 and CCL22 facilitating chemotaxis of immune cells to sites of inflammation. Researchers often refer to antibodies targeting CCR4 like mogamulizumab when studying immune responses.
- Biological function summary
CCR4 plays an important role in the trafficking of immune cells to inflammatory sites therefore regulating immune response and tolerance. It is part of the chemokine receptor family which is integral to the immune system's function. CCR4 helps maintain the balance between effector and regulatory T cells contributing to immunological homeostasis. Its involvement in migration and positioning of immune cells within lymphoid and peripheral tissues highlights its importance in maintaining immune surveillance.
- Pathways
CCR4 significantly influences the chemokine signaling pathway which is pivotal in directing the movement and positioning of immune cells. This receptor interacts with key proteins like CCL17 and CCL22 to mediate cell chemotaxis in these pathways. Additionally CCR4 is associated with the T-cell receptor signaling pathway where it coordinates with other receptors and proteins to modulate immune responses.
- Associated diseases and disorders
Increased CCR4 expression has connections with various conditions such as adult T-cell leukemia/lymphoma and cutaneous T-cell lymphomas. In these diseases CCR4 assists tumor cells in evading the immune system fueling disease progression. Proteins like TARC (thymus and activation-regulated chemokine) and MDC (macrophage-derived chemokine) ligands for CCR4 often facilitate tumor cell migration and proliferation. Therapeutic approaches targeting CCR4 such as the anti-CCR4 antibody mogamulizumab have shown potential in treating these malignancies by disrupting these interactions.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
4 product images
![Western blot - Anti-CCR4 antibody [EPR26522-156] (ab315446), expandable thumbnail](https://content.abcam.com/products/images/ccr4-antibody-epr26522-156-ab315446--western-blot-img425121.jpg "Western blot - Anti-CCR4 antibody [EPR26522-156] (ab315446)")
Western blot - Anti-CCR4 antibody [EPR26522-156] (ab315446)
CCR4 Western blot staining using rabbit Anti-CCR4 antibody
Low expression: HT-29 (PMID: 28645139).
Samples are non-boiled as boiling may cause protein aggregation.
In Western blot, Anti-Vinculin antibody [EPR8185] (Anti-Vinculin antibody [EPR8185] - Loading Control ab129002) staining at 1/10000 dilution.
All lanes: Western blot - Anti-CCR4 antibody [EPR26522-156] (ab315446) at 1/1000 dilution
Lane 1: HuT-78 (human Sezary syndrome cutaneous T lymphocyte) whole cell lysate at 20 µg
Lane 2: HT-29 (human colorectal adenocarcinoma epithelial cell) whole cell lysate at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Observed band size: 37 kDa, 124 kDa
Exposure time: 48s
![Western blot - Anti-CCR4 antibody [EPR26522-156] (ab315446), expandable thumbnail](https://content.abcam.com/products/images/ccr4-antibody-epr26522-156-ab315446--western-blot-img425120.jpg "Western blot - Anti-CCR4 antibody [EPR26522-156] (ab315446)")
Western blot - Anti-CCR4 antibody [EPR26522-156] (ab315446)
CCR4 Western blot staining using rabbit Anti-CCR4 antibody
Negative control: 293T(PMID: 22973452).
Samples are non-boiled as boiling may cause protein aggregation.
In Western blot, Anti-Vinculin antibody [EPR8185] (Anti-Vinculin antibody [EPR8185] - Loading Control ab129002) staining at 1/10000 dilution.
All lanes: Western blot - Anti-CCR4 antibody [EPR26522-156] (ab315446) at 1/1000 dilution
Lane 1: HDLM-2 (human Hodgkin lymphoma cell) whole cell lysate at 20 µg
Lane 2: 293T (human embryonic kidney epithelial cell) whole cell lysate at 20 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
Observed band size: 37 kDa, 124 kDa
Exposure time: 48s
![Flow Cytometry - Anti-CCR4 antibody [EPR26522-156] (ab315446), expandable thumbnail](https://content.abcam.com/products/images/ccr4-antibody-epr26522-156-ab315446--flow-cytometry-img425243.jpg "Flow Cytometry - Anti-CCR4 antibody [EPR26522-156] (ab315446)")
Flow Cytometry - Anti-CCR4 antibody [EPR26522-156] (ab315446)
Flow cytometric analysis of Human PBMC (human peripheral blood mononuclear cell) cells labelling CCR4 with ab315446 at 1/50 dilution (1 ug)/Right compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) / Left isotype control.
Goat Anti-Rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/5000 dilution was used as the secondary antibody.
Gated on viable cells. Cells are co-stained with CD3 conjugated to Alexa Fluor®647.
![Flow Cytometry - Anti-CCR4 antibody [EPR26522-156] (ab315446), expandable thumbnail](https://content.abcam.com/products/images/ccr4-antibody-epr26522-156-ab315446--flow-cytometry-img425242.jpg "Flow Cytometry - Anti-CCR4 antibody [EPR26522-156] (ab315446)")
Flow Cytometry - Anti-CCR4 antibody [EPR26522-156] (ab315446)
Flow cytometric analysis of 293T (human embryonic kidney epithelial cell, Left) / HDLM-2 (human Hodgkin lymphoma cell, Right) cells labelling CCR4 with ab315446 at 1/50 dilution (1 ug)/Red compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).
Goat Anti-Rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/5000 dilution was used as the secondary antibody.
Negative control: 293T. Gated on viable cells.
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