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Rabbit Recombinant Monoclonal CCR4 antibody. Suitable for Flow Cyt, ICC/IF and reacts with Transfected cell line - Mouse, Mouse samples.

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Images

Flow Cytometry - Anti-CCR4 antibody [EPR27421-23] (AB318958), expandable thumbnail
  • Immunocytochemistry/ Immunofluorescence - Anti-CCR4 antibody [EPR27421-23] (AB318958), expandable thumbnail
  • Flow Cytometry - Anti-CCR4 antibody [EPR27421-23] (AB318958), expandable thumbnail
  • Flow Cytometry - Anti-CCR4 antibody [EPR27421-23] (AB318958), expandable thumbnail

Key facts

Isotype

IgG

Host species

Rabbit

Storage buffer

pH: 7.2 - 7.4
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Form

Liquid

Clonality

Monoclonal

Immunogen

  • The exact immunogen used to generate this antibody is proprietary information.

Reactivity data

Select an application
Product promiseTestedExpectedPredictedNot recommended
Flow CytICC/IFWB
Human
Not recommended
Not recommended
Not recommended
Mouse
Tested
Tested
Not recommended
Rat
Not recommended
Not recommended
Not recommended
Transfected cell line - Mouse
Tested
Not recommended
Not recommended

Tested
Tested

Species

Transfected cell line - Mouse

Dilution info

1/5000

Notes

-

Species

Mouse

Dilution info

1/50

Notes

-

Not recommended
Not recommended

Species

Human, Rat

Dilution info

-

Notes

-

Tested
Tested

Species

Mouse

Dilution info

1/50

Notes

-

Not recommended
Not recommended

Species

Transfected cell line - Mouse, Human, Rat

Dilution info

-

Notes

-

Not recommended
Not recommended

Species

Human, Mouse, Rat, Transfected cell line - Mouse

Dilution info

-

Notes

-

Target data

Function

High affinity receptor for the C-C type chemokines CCL17/TARC and CCL22/MDC. The activity of this receptor is mediated by G(i) proteins which activate a phosphatidylinositol-calcium second messenger system. Could play a role in lipopolysaccharide (LPS)-induced endotoxic shock. In the CNS, could mediate hippocampal-neuron survival.

Alternative names

C-C chemokine receptor type 4, C-C CKR-4, CC-CKR-4, CCR-4, CCR4

Recommended products

Rabbit Recombinant Monoclonal CCR4 antibody. Suitable for Flow Cyt, ICC/IF and reacts with Transfected cell line - Mouse, Mouse samples.

Alternative names

C-C chemokine receptor type 4, C-C CKR-4, CC-CKR-4, CCR-4, CCR4

Key facts

Isotype

IgG

Form

Liquid

Clonality

Monoclonal

Immunogen
  • The exact immunogen used to generate this antibody is proprietary information.
Clone number

EPR27421-23

Purification technique

Affinity purification Protein A

Concentration
Loading...

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Notes

Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

CCR4 also known as C-C chemokine receptor type 4 is a G protein-coupled receptor with the molecular mass of approximately 41 kDa. It is predominantly expressed on the surface of T-helper 2 cells regulatory T cells and certain dendritic cells. CCR4 functions by binding chemokines such as CCL17 and CCL22 facilitating chemotaxis of immune cells to sites of inflammation. Researchers often refer to antibodies targeting CCR4 like mogamulizumab when studying immune responses.

Biological function summary

CCR4 plays an important role in the trafficking of immune cells to inflammatory sites therefore regulating immune response and tolerance. It is part of the chemokine receptor family which is integral to the immune system's function. CCR4 helps maintain the balance between effector and regulatory T cells contributing to immunological homeostasis. Its involvement in migration and positioning of immune cells within lymphoid and peripheral tissues highlights its importance in maintaining immune surveillance.

Pathways

CCR4 significantly influences the chemokine signaling pathway which is pivotal in directing the movement and positioning of immune cells. This receptor interacts with key proteins like CCL17 and CCL22 to mediate cell chemotaxis in these pathways. Additionally CCR4 is associated with the T-cell receptor signaling pathway where it coordinates with other receptors and proteins to modulate immune responses.

Associated diseases and disorders

Increased CCR4 expression has connections with various conditions such as adult T-cell leukemia/lymphoma and cutaneous T-cell lymphomas. In these diseases CCR4 assists tumor cells in evading the immune system fueling disease progression. Proteins like TARC (thymus and activation-regulated chemokine) and MDC (macrophage-derived chemokine) ligands for CCR4 often facilitate tumor cell migration and proliferation. Therapeutic approaches targeting CCR4 such as the anti-CCR4 antibody mogamulizumab have shown potential in treating these malignancies by disrupting these interactions.

Product promise

We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.

In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

4 product images

  • Flow Cytometry - Anti-CCR4 antibody [EPR27421-23] (ab318958), expandable thumbnail

    Flow Cytometry - Anti-CCR4 antibody [EPR27421-23] (ab318958)

    Flow cytometric analysis of 2% paraformaldehyde fixed 0.1% Tween-20 permeabilized Isotype (Left) / 293T cells transfected with a mouse CCR4 expression vector containing a myc-His-tag® (Middle) / 293T cells transfected with an empty expression vector containing a myc-His-tag® (Right) cells labelling CCR4 with ab318958 at 1/5000 dilution (0.01 ug)/Middle and Right (Red) compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

    Goat Anti-Rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/5000 dilution was used as the secondary antibody.

    Cells were surface stained with isotype control (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) or ab318958. Then fixed with 2% PFA for 10min followed by intracellularly stained with anti-myc tag conjugated to Alexa Fluor® 647.

  • Immunocytochemistry/ Immunofluorescence - Anti-CCR4 antibody [EPR27421-23] (ab318958), expandable thumbnail

    Immunocytochemistry/ Immunofluorescence - Anti-CCR4 antibody [EPR27421-23] (ab318958)

    Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized Mouse thymocyte cells labelling CCR4 with ab318958 at 1/50 (10.28 ug/ml) dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2 ug/ml dilution (Green).

    Confocal image showing cytoplasmic staining in subsets of mouse thymocytes (shown in green). The counterstain was observed in magenta. Nuclear DNA was labeled with DAPI (shown in blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

    Alexa Fluor® 594 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

    Secondary antibody only control: Secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/ml dilution.

  • Flow Cytometry - Anti-CCR4 antibody [EPR27421-23] (ab318958), expandable thumbnail

    Flow Cytometry - Anti-CCR4 antibody [EPR27421-23] (ab318958)

    Flow cytometric analysis of Mouse thymocyte cells labelling CCR4 with ab318958 at 1/50 dilution (1 ug)/Right compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

    Goat Anti-Rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/5000 dilution was used as the secondary antibody.

    Mouse thymocyte are co-stained with CD4 conjugated APC/Fire™ 750 (780/60BP).

    Gated on viable cell.

  • Flow Cytometry - Anti-CCR4 antibody [EPR27421-23] (ab318958), expandable thumbnail

    Flow Cytometry - Anti-CCR4 antibody [EPR27421-23] (ab318958)

    Flow cytometric analysis of Mouse thymocyte cells labelling CCR4 with ab318958 at 1/50 dilution (1 ug)/Right compared with a Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

    Goat Anti-Rabbit IgG (Alexa Fluor® 488, Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed ab150081) at 1/5000 dilution was used as the secondary antibody.

    Mouse thymocyte are co-stained with NK1.1 conjugated Alexa Fluor®647 (670/14BP).

    Gated on viable cell.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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