Anti-CCR4 antibody [EPR27421-23] - BSA and Azide free

• Flow Cyt

Supplier Data

Flow Cytometry - Anti-CCR4 antibody [EPR27421-23] - BSA and Azide free (AB318959)

This data was developed using ab318958, the same antibody clone in a different buffer formulation.

Flow cytometric analysis of 2% paraformaldehyde fixed 0.1% Tween-20 permeabilized Isotype (Left) / 293T cells transfected with a mouse CCR4 expression vector containing a myc-His-tag® (Middle) / 293T cells transfected with an empty expression vector containing a myc-His-tag® (Right) cells labelling CCR4 with ab318958 at 1/5000 dilution (0.01 ug)/Middle and Right (Red) compared with a Rabbit monoclonal IgG (ab172730) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/5000 dilution was used as the secondary antibody.

Cells were surface stained with isotype control (ab172730) or ab318958. Then fixed with 2% PFA for 10min followed by intracellularly stained with anti-myc tag conjugated to Alexa Fluor® 647.

• Flow Cyt

Supplier Data

Flow Cytometry - Anti-CCR4 antibody [EPR27421-23] - BSA and Azide free (AB318959)

This data was developed using ab318958, the same antibody clone in a different buffer formulation.

Flow cytometric analysis of Mouse thymocyte cells labelling CCR4 with ab318958 at 1/50 dilution (1 ug)/Right compared with a Rabbit monoclonal IgG (ab172730) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/5000 dilution was used as the secondary antibody.

Mouse thymocyte are co-stained with CD4 conjugated APC/Fire™ 750 (780/60BP).
Gated on viable cell.

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-CCR4 antibody [EPR27421-23] - BSA and Azide free (AB318959)

This data was developed using ab318958, the same antibody clone in a different buffer formulation.

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized Mouse thymocyte cells labelling CCR4 with ab318958 at 1/50 (10.28 ug/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2 ug/ml dilution (Green).

Confocal image showing cytoplasmic staining in subsets of mouse thymocytes (shown in green). The counterstain was observed in magenta. Nuclear DNA was labeled with DAPI (shown in blue). Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).

ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Magenta). The Nuclear counterstain was DAPI (Blue).

Secondary antibody only control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2 ug/ml dilution.

• Flow Cyt

Supplier Data

Flow Cytometry - Anti-CCR4 antibody [EPR27421-23] - BSA and Azide free (AB318959)

This data was developed using ab318958, the same antibody clone in a different buffer formulation.

Flow cytometric analysis of Mouse thymocyte cells labelling CCR4 with ab318958 at 1/50 dilution (1 ug)/Right compared with a Rabbit monoclonal IgG (ab172730) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue).

Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/5000 dilution was used as the secondary antibody.

Mouse thymocyte are co-stained with NK1.1 conjugated Alexa Fluor®647 (670/14BP).
Gated on viable cell.