AB212302

Anti-CCR5 antibody [12D1] - BSA and Azide free

Name: Anti-CCR5 antibody [12D1] - BSA and Azide free (ab212302) | Abcam
Brand: Abcam Limited
SKU: AB212302
Price: 905 USD
Availability: InStock

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(1 Publication)

Mouse Monoclonal CCR5 antibody. Carrier free. Suitable for IHC-Fr and reacts with Human samples. Cited in 1 publication. Immunogen corresponding to Native Full Length Protein corresponding to Human CCR5.

View Alternative Names

CD195, CMKBR5, CCR5, C-C chemokine receptor type 5, C-C CKR-5, CC-CKR-5, CCR-5, CHEMR13, HIV-1 fusion coreceptor

1 Images

Immunohistochemistry (Frozen sections) - Anti-CCR5 antibody [12D1] - BSA and Azide free (AB212302)

IHC-Fr

Supplier Data

Immunohistochemistry (Frozen sections) - Anti-CCR5 antibody [12D1] - BSA and Azide free (AB212302)

Frozen human stomach tissue stained for CCR5 using ab212302 at 1 μg/ml in immnohistochemical analysis.

This IHC-Fr data was generated using the same anti-CCR5 antibody clone, 12D1, in a different buffer formulation.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

12D1

Isotype

IgG2a

Light chain type

kappa

Carrier free

Yes

Reacts with

Human

Applications

IHC-Fr

applications

Immunogen

Native Full Length Protein corresponding to Human CCR5.

P51681

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCFr-species-checked": "testedAndGuaranteed", "IHCFr-species-dilution-info": "0.5-1 µg/mL", "IHCFr-species-notes": "<p>(Primary incubation for 30 minutes at RT).</p>" }, "Chimpanzee": { "IHCFr-species-checked": "predicted", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" }, "Cynomolgus monkey": { "IHCFr-species-checked": "predicted", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" }, "Gorilla": { "IHCFr-species-checked": "predicted", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" }, "Orangutan": { "IHCFr-species-checked": "predicted", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" }, "Rhesus monkey": { "IHCFr-species-checked": "predicted", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A/G

Purification notes

ab212302 was purified from bioreactor concentrate by protein A/G.

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CCR5 also known as the C-C chemokine receptor type 5 is a protein that functions as a receptor on the surface of cells predominantly immune cells like T-cells macrophages and dendritic cells. The approximate molecular weight of CCR5 is 40 kDa. This protein belongs to the G-protein-coupled receptor family and it mainly binds to β-chemokines. CCR5 is abundantly expressed in immune tissues such as the spleen thymus and lymph nodes. Researchers often use assays like the CCR5 ELISA kit to detect or quantify its presence in various samples.

Biological function summary

CCR5 acts as a receptor for various chemokines specifically CCL3 CCL4 and CCL5 which are involved in leukocyte trafficking and immune response modulation. It plays an important role in the migration of immune cells to sites of inflammation or injury. This protein does not function in isolation; it sometimes forms part of larger complexes with other cell surface proteins to mediate responses to external signals. For example anti-CCR5 antibodies like 12D1 can modulate its activity and influence immune cell behavior.

Pathways

CCR5 is intricately involved in chemokine signaling pathways and inflammatory response pathways. Through these pathways it interacts with proteins such as CXCR4 and CCR1. CCR5 and CXCR4 another chemokine receptor are well-documented for their role in HIV entry into host cells. These pathways are critical for orchestrating immune cell movement and activating signaling cascades that promote immune defense mechanisms.

CCR5 has a significant association with HIV infection and is a known co-receptor facilitating the virus's entry into immune cells. The presence of mutations like the Δ32 allele in the CCR5 gene provides resistance against HIV infection highlighting its importance in disease dynamics. CCR5 also plays a role in inflammatory diseases such as rheumatoid arthritis where it contributes to the migration of immune cells into joints exacerbating inflammation. In these contexts CCR5 interacts with other inflammatory mediators offering potential therapeutic targets for drug development.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Receptor for a number of inflammatory CC-chemokines including CCL3/MIP-1-alpha, CCL4/MIP-1-beta and RANTES and subsequently transduces a signal by increasing the intracellular calcium ion level. May play a role in the control of granulocytic lineage proliferation or differentiation. Participates in T-lymphocyte migration to the infection site by acting as a chemotactic receptor (PubMed : 30713770).. (Microbial infection) Acts as a coreceptor (CD4 being the primary receptor) of human immunodeficiency virus-1/HIV-1.

See full target information CCR5

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

International journal of molecular sciences 20: PubMed31035427

2019

Mapping Semaphorins and Netrins in the Pathogenesis of Human Thoracic Aortic Aneurysms.

Applications

Unspecified application

Species

Unspecified reactive species

Dornazsadat Alebrahim,Mangala Nayak,Alison Ward,Patricia Ursomanno,Rebecca Shams,Annanina Corsica,Rayan Sleiman,Kissinger Hyppolite Fils,Michele Silvestro,Ludovic Boytard,Tarik Hadi,Bruce Gelb,Bhama Ramkhelawon

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-CCR5 antibody [12D1] - BSA and Azide free

Immunohistochemistry (Frozen sections) - Anti-CCR5 antibody [12D1] - BSA and Azide free (AB212302)

Key facts

Host species

Clonality

Clone number

Isotype

Light chain type

Carrier free

Reacts with

Applications

Immunogen

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Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Product promise