Anti-CCR5 antibody [EPR29865-504]

• WB

Lab

Western blot - Anti-CCR5 antibody [EPR29865-504] (AB326722)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

This blot was developed using a high-sensitivity ECL substrate, allowing for the detection of proteins in the mid-femtogram range.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) (1 : 200000) (36KDa).

All lanes:

Western blot - Anti-CCR5 antibody [EPR29865-504] (ab326722) at 1/1000 dilution

Lane 1:

Human tonsil tissue lysate at 20 µg

Lane 2:

Human lymph node tissue lysate at 20 µg

Secondary

All lanes:

Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

Observed band size: 41-70 kDa,36 kDa

true

Exposure time: 180s

• WB

Lab

Western blot - Anti-CCR5 antibody [EPR29865-504] (AB326722)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

This antibody does not cross-react with overexpressed human CCR2 by western blot.

In Western blot, Anti-6X His tag® antibody [EPR20547] - ChIP Grade (ab213204) (1 : 5000) (41KDa); Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) (1 : 200000) (36KDa).

All lanes:

Western blot - Anti-CCR5 antibody [EPR29865-504] (ab326722) at 1/1000 dilution

Lane 1:

293T (human embryonic kidney epithelial cell) cells transfected with an empty vector containing a myc-His-tag® whole cell lysate at 20 µg

Lane 2:

293T cells transfected with human CCR5 expression vector containing a myc-His-tag® whole cell lysate at 20 µg

Lane 3:

293T cells transfected with human CCR2 expression vector containing a myc-His-tag® whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 41-70 kDa,41 kDa,36 kDa

false

Exposure time: 180s

• WB

Lab

Western blot - Anti-CCR5 antibody [EPR29865-504] (AB326722)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Low expression : A375.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) (1 : 200000) (36KDa).

All lanes:

Western blot - Anti-CCR5 antibody [EPR29865-504] (ab326722) at 1/1000 dilution

Lane 1:

HuT-78 (human sezary syndrome cutaneous t lymphocyte) whole cell lysate at 20 µg

Lane 2:

THP-1 (human monocytic leukemia monocyte) whole cell lysate at 20 µg

Lane 3:

A375 (human malignant melanoma epithelial cell) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 41-70 kDa,36 kDa

false

Exposure time: 180s

• WB

Lab

Western blot - Anti-CCR5 antibody [EPR29865-504] (AB326722)

Blocking and diluting buffer and concentration : 5% NFDM/TBST.

Low expression : HepG2, HeLa, A431.

CCR5 is a glycoprotein of approximately 70 kDa and detected as a 41kDa band after treated with Protein Deglycosylation MIX II.

In Western blot, Anti-GAPDH antibody [EPR16891] - Loading Control (ab181602) (1 : 200000) (36KDa).

All lanes:

Western blot - Anti-CCR5 antibody [EPR29865-504] (ab326722) at 1/1000 dilution

Lane 1:

Untreated KG-1a (human bone marrow myeloblast) whole cell lysate at 20 µg

Lane 2:

KG-1a whole cell lysate treated with Protein Deglycosylation Mix II at 20 µg

Lane 3:

HepG2 (human hepatocellular carcinoma epithelial cell) whole cell lysate at 20 µg

Lane 4:

HeLa (human cervical adenocarcinoma epithelial cell) whole cell lysate at 20 µg

Lane 5:

A431 (human epidermoid carcinoma epithelial cell) whole cell lysate at 20 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-ab97051'>ab97051</a>) at 1/20000 dilution

Observed band size: 41-70 kDa,36 kDa

false

Exposure time: 180s