Anti-CCR5 antibody [HL3650] - BSA and Azide free

• ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-CCR5 antibody [HL3650] - BSA and Azide free (AB325843)

ab325843 detects CCR5 protein by immunofluorescent analysis. Sample : THP-1 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green : CCR5 stained by ab325843 diluted at 1 : 100. Blue : Fluoroshield with DAPI.

• WB

Supplier Data

Western blot - Anti-CCR5 antibody [HL3650] - BSA and Azide free (AB325843)

Rat tissue extract (50 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with ab325843 diluted at 1 : 1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

All lanes:

Western blot - Anti-CCR5 antibody [HL3650] - BSA and Azide free (ab325843) at 1/1000 dilution

All lanes:

Rat spleen tissue extract at 50 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

false

• WB

Supplier Data

Western blot - Anti-CCR5 antibody [HL3650] - BSA and Azide free (AB325843)

Unboiled non-transfected (–) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with ab325843 diluted at 1 : 5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

All lanes:

Western blot - Anti-CCR5 antibody [HL3650] - BSA and Azide free (ab325843) at 1/5000 dilution

Lanes 1, 3, 5, 7, 9, 11 and 13:

Unboiled non-transfected (–) 293T whole cell extracts at 30 µg

Lane 2:

Unboiled CCR1 transfected (+) 293T whole cell extracts at 30 µg

Lane 4:

Unboiled CCR2 transfected (+) 293T whole cell extracts at 30 µg

Lane 6:

Unboiled CCR4 transfected (+) 293T whole cell extracts at 30 µg

Lane 8:

Unboiled CCR5 transfected (+) 293T whole cell extracts at 30 µg

Lane 10:

Unboiled CCR7 transfected (+) 293T whole cell extracts at 30 µg

Lane 12:

Unboiled CCR9 transfected (+) 293T whole cell extracts at 30 µg

Lane 14:

Unboiled CCR10 transfected (+) 293T whole cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

false

• WB

Supplier Data

Western blot - Anti-CCR5 antibody [HL3650] - BSA and Azide free (AB325843)

Non-transfected (–) and transfected (+) boiled and unboiled 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with ab325843 diluted at 1 : 5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

All lanes:

Western blot - Anti-CCR5 antibody [HL3650] - BSA and Azide free (ab325843) at 1/5000 dilution

Lane 1:

Non-transfected (–) boiled 293T whole cell extracts at 30 µg

Lane 2:

DDDDK-tagged CCR5 transfected (+) boiled 293T whole cell extracts at 30 µg

Lane 3:

Non-transfected (–) unboiled 293T whole cell extracts at 30 µg

Lane 4:

DDDDK-tagged CCR5 transfected (+) unboiled 293T whole cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

false