AB110103

Anti-CCR5 antibody [T21/8]

Recombinant
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(5 Publications)

Mouse Recombinant Monoclonal CCR5 antibody. Suitable for Flow Cyt, I-ELISA and reacts with Human samples. Cited in 5 publications.

View Alternative Names

CD195, CMKBR5, CCR5, C-C chemokine receptor type 5, C-C CKR-5, CC-CKR-5, CCR-5, CHEMR13, HIV-1 fusion coreceptor

2 Images

Flow Cytometry - Anti-CCR5 antibody [T21/8] (AB110103)

Flow Cyt

Lab

Flow Cytometry - Anti-CCR5 antibody [T21/8] (AB110103)

Flow cytometric analysis of Human peripheral blood mononuclear cells (PBMC) cells labelling CCR5 with ab110103 at 1/100 dilution (0.1&micor;g)/Right followed by a secondary antibody Goat anti-Mouse IgG (Alexa Fluor^® 488, ab150113) used at a 1/2000 dilution compared with isotype control Mouse monoclonal IgG / Left.

Cells were stained with mouse IgG or ab110103. Then stained with anti-CD3 conjugated to APC (the conjugation was performed by using ab240074 and APC Conjugation Kit - Lightning-Link^® (ab201807)).
Gated on viable lymphocytes.

Indirect ELISA - Anti-CCR5 antibody [T21/8] (AB110103)

I-ELISA

Lab

Indirect ELISA - Anti-CCR5 antibody [T21/8] (AB110103)

Indirect ELISA analysis of ab110103 used at a range 0-2000 ng/ml. Antigen used was Human CCR5 at 1000 ng/ml. The Secondary antibody used was Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Mouse IgG (H+L) at 1/1000 dilution dilution.

660 APC

APC Anti-CCR5 antibody [T21/8]
519 Alexa Fluor® 488

Alexa Fluor® 488 Anti-CCR5 antibody [T21/8]
387 mFluor™ UV375

mFluor™ UV375 Anti-CD195 antibody [T21/8]
454 mFluor™ Violet 450

mFluor™ Violet 450 Anti-CD195 antibody [T21/8]

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

T21/8

Isotype

IgG1

Light chain type

kappa

Carrier free

Reacts with

Human

Applications

I-ELISA, Flow Cyt

applications

Epitope

The antibody reacts with the amino terminus of the protein. The exact amino acids that comprise the epitope recognized by the antibody have not been determined.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "IELISA" : {"fullname" : "Indirect ELISA", "shortname":"I-ELISA"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "20 µg/mL", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1/100", "FlowCyt-species-notes": "<a href='/en-us/products/primary-antibodies/mouse-igg1-kappa-monoclonal-15-6e10a7-isotype-control-ab170190'>ab170190</a> - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.", "IELISA-species-checked": "testedAndGuaranteed", "IELISA-species-dilution-info": "", "IELISA-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Mouse": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "20 µg/mL", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "IELISA-species-checked": "notRecommended", "IELISA-species-dilution-info": "", "IELISA-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "" }, "Rat": { "IHCP-species-checked": "notRecommended", "IHCP-species-dilution-info": "20 µg/mL", "IHCP-species-notes": "", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "IELISA-species-checked": "notRecommended", "IELISA-species-dilution-info": "", "IELISA-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "" } } }

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Product details

The production method has been switched from hybridoma to recombinant on 06th June 2025.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

- High batch-to-batch consistency and reproducibility
- Improved sensitivity and specificity
- Long-term security of supply
- Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein A

Storage buffer

pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CCR5 also known as the C-C chemokine receptor type 5 is a protein that functions as a receptor on the surface of cells predominantly immune cells like T-cells macrophages and dendritic cells. The approximate molecular weight of CCR5 is 40 kDa. This protein belongs to the G-protein-coupled receptor family and it mainly binds to β-chemokines. CCR5 is abundantly expressed in immune tissues such as the spleen thymus and lymph nodes. Researchers often use assays like the CCR5 ELISA kit to detect or quantify its presence in various samples.

Biological function summary

CCR5 acts as a receptor for various chemokines specifically CCL3 CCL4 and CCL5 which are involved in leukocyte trafficking and immune response modulation. It plays an important role in the migration of immune cells to sites of inflammation or injury. This protein does not function in isolation; it sometimes forms part of larger complexes with other cell surface proteins to mediate responses to external signals. For example anti-CCR5 antibodies like 12D1 can modulate its activity and influence immune cell behavior.

Pathways

CCR5 is intricately involved in chemokine signaling pathways and inflammatory response pathways. Through these pathways it interacts with proteins such as CXCR4 and CCR1. CCR5 and CXCR4 another chemokine receptor are well-documented for their role in HIV entry into host cells. These pathways are critical for orchestrating immune cell movement and activating signaling cascades that promote immune defense mechanisms.

CCR5 has a significant association with HIV infection and is a known co-receptor facilitating the virus's entry into immune cells. The presence of mutations like the Δ32 allele in the CCR5 gene provides resistance against HIV infection highlighting its importance in disease dynamics. CCR5 also plays a role in inflammatory diseases such as rheumatoid arthritis where it contributes to the migration of immune cells into joints exacerbating inflammation. In these contexts CCR5 interacts with other inflammatory mediators offering potential therapeutic targets for drug development.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Receptor for a number of inflammatory CC-chemokines including CCL3/MIP-1-alpha, CCL4/MIP-1-beta and RANTES and subsequently transduces a signal by increasing the intracellular calcium ion level. May play a role in the control of granulocytic lineage proliferation or differentiation. Participates in T-lymphocyte migration to the infection site by acting as a chemotactic receptor (PubMed : 30713770).. (Microbial infection) Acts as a coreceptor (CD4 being the primary receptor) of human immunodeficiency virus-1/HIV-1.

See full target information CCR5

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Publications (5)

Recent publications for all applications. Explore the full list and refine your search

Advanced science (Weinheim, Baden-Wurttemberg, Germany) 10:e2302527 PubMed37867250

2023

Enabling Survival of Transplanted Neural Precursor Cells in the Ischemic Brain.

Applications

Unspecified application

Species

Unspecified reactive species

Zhifu Wang,Danyi Zheng,Ye Sing Tan,Qiang Yuan,Fang Yuan,Su-Chun Zhang

Journal for immunotherapy of cancer 11: PubMed37185233

2023

Chordoma recruits and polarizes tumor-associated macrophages via secreting CCL5 to promote malignant progression.

Applications

Unspecified application

Species

Unspecified reactive species

Jiuhui Xu,Qianyu Shi,Jingbing Lou,Boyang Wang,Wei Wang,Jianfang Niu,Lei Guo,Chenglong Chen,Yiyang Yu,Yi Huang,Wei Guo,Jianqiang Lan,Yu Zhu,Tingting Ren,Xiaodong Tang

Frontiers in pharmacology 12:551839 PubMed33953665

2021

miR-4456/CCL3/CCR5 Pathway in the Pathogenesis of Tight Junction Impairment in Chronic Obstructive Pulmonary Disease.

Applications

Unspecified application

Species

Unspecified reactive species

Weiwei Yu,Ting Ye,Jie Ding,Yi Huang,Yang Peng,Qin Xia,Zhang Cuntai

Experimental dermatology 28:816-821 PubMed31006151

2019

Expression and biological functions of the CCL5-CCR5 axis in oral lichen planus.

Applications

Unspecified application

Species

Unspecified reactive species

Jing Shan,Shan Li,Chen Wang,Lin Liu,Xuewei Wang,Dandan Zhu,Yuan Fan,Juanyong Xu

Oncology letters 13:3379-3386 PubMed28521443

2017

Loss of Fas expression and high expression of HLA-E promoting the immune escape of early colorectal cancer cells.

Applications

Unspecified application

Species

Unspecified reactive species

Renxiang Huang,Dongyang Zhang,Feng Li,Zili Xiao,Meiling Wu,Dongyun Shi,Ping Xiang,Zhijun Bao

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-CCR5 antibody [T21/8]

Flow Cytometry - Anti-CCR5 antibody [T21/8] (AB110103)

Indirect ELISA - Anti-CCR5 antibody [T21/8] (AB110103)

Related conjugates and formulations (4)

Key facts

Host species

Clonality

Clone number

Isotype

Light chain type

Carrier free

Reacts with

Applications

Epitope

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Reactivity data

Product details

Properties and storage information

Form

Purification technique

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (5)

Product promise