Rabbit Recombinant Monoclonal CCR6 antibody. Carrier free. Suitable for I-ELISA, IP, WB, IHC-P and reacts with Synthetic peptide - Human, Human samples. Cited in 2 publications.
Images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CCR6 antibody [EPR22259] - BSA and Azide free (AB243852), expandable thumbnail](https://content.abcam.com/products/images/ccr6-antibody-epr22259-bsa-and-azide-free-ab243852--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img48166.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CCR6 antibody [EPR22259] - BSA and Azide free (AB243852)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CCR6 antibody [EPR22259] - BSA and Azide free (AB243852), expandable thumbnail](https://content.abcam.com/products/images/ccr6-antibody-epr22259-bsa-and-azide-free-ab243852--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img81213.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CCR6 antibody [EPR22259] - BSA and Azide free (AB243852)")
![Immunoprecipitation - Anti-CCR6 antibody [EPR22259] - BSA and Azide free (AB243852), expandable thumbnail](https://content.abcam.com/products/images/ccr6-antibody-epr22259-bsa-and-azide-free-ab243852--immunoprecipitation-img11835.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-CCR6 antibody [EPR22259] - BSA and Azide free (AB243852)")
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CCR6 antibody [EPR22259] - BSA and Azide free (AB243852), expandable thumbnail](https://content.abcam.com/products/images/ccr6-antibody-epr22259-bsa-and-azide-free-ab243852--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img423091.jpg/_jcr_content/renditions/webp-475.webp "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CCR6 antibody [EPR22259] - BSA and Azide free (AB243852)")
![Indirect ELISA - Anti-CCR6 antibody [EPR22259] - BSA and Azide free (AB243852), expandable thumbnail](https://content.abcam.com/products/images/ccr6-antibody-epr22259-bsa-and-azide-free-ab243852--indirect-elisa-img417708.jpg/_jcr_content/renditions/webp-475.webp "Indirect ELISA - Anti-CCR6 antibody [EPR22259] - BSA and Azide free (AB243852)")
Publications
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- Frontiers in immunology 14:11626692023Naproxen chemoprevention induces proliferation of cytotoxic lymphocytes in Lynch Syndrome colorectal mucosa.Applications:Unspecified applicationReactive species:Unspecified reactive speciesCharles M Bowen,Nan Deng,Laura Reyes-Uribe,Edwin Roger Parra,Pedro Rocha,Luisa M Solis,Ignacio I Wistuba,Valerie O Sepeda,Lana Vornik,Marjorie Perloff,Eva Szabo,Asad Umar,Krishna M Sinha,Powel H Brown,Eduardo VilarPubMed 37207208
- Cell 184:4996-5014.e262021Antigen dominance hierarchies shape TCF1 progenitor CD8 T cell phenotypes in tumors.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMegan L Burger,Amanda M Cruz,Grace E Crossland,Giorgio Gaglia,Cecily C Ritch,Sarah E Blatt,Arjun Bhutkar,David Canner,Tamina Kienka,Sara Z Tavana,Alexia L Barandiaran,Andrea Garmilla,Jason M Schenkel,Michelle Hillman,Izumi de Los Rios Kobara,Amy Li,Alex M Jaeger,William L Hwang,Peter M K Westcott,Michael P Manos,Marta M Holovatska,F Stephen Hodi,Aviv Regev,Sandro Santagata,Tyler JacksPubMed 34534464
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| I-ELISA | IP | Flow Cyt | WB | ICC/IF | IHC-P | |
|---|---|---|---|---|---|---|
| Human | Expected | Tested | Not recommended | Expected | Not recommended | Tested |
| Synthetic peptide - Human | Tested | Not recommended | Not recommended | Not recommended | Not recommended | Not recommended |
I-ELISA
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Synthetic peptide - Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Synthetic peptide - Human | Dilution info - | Notes - |
Flow Cyt
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Synthetic peptide - Human | Dilution info - | Notes - |
WB
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Synthetic peptide - Human | Dilution info - | Notes - |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Synthetic peptide - Human | Dilution info - | Notes - |
IHC-P
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes Perform heat-mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Synthetic peptide - Human | Dilution info - | Notes - |
Associated Products
Select an associated product type
3 products for Alternative Product
Target data
Function
Receptor for the C-C type chemokine CCL20 (PubMed:9169459). Binds to CCL20 and subsequently transduces a signal by increasing the intracellular calcium ion levels (PubMed:20068036). Although CCL20 is its major ligand it can also act as a receptor for non-chemokine ligands such as beta-defensins (PubMed:25585877). Binds to defensin DEFB1 leading to increase in intracellular calcium ions and cAMP levels. Its binding to DEFB1 is essential for the function of DEFB1 in regulating sperm motility and bactericidal activity (PubMed:25122636). Binds to defensins DEFB4 and DEFB4A/B and mediates their chemotactic effects (PubMed:20068036). The ligand-receptor pair CCL20-CCR6 is responsible for the chemotaxis of dendritic cells (DC), effector/ memory T-cells and B-cells and plays an important role at skin and mucosal surfaces under homeostatic and inflammatory conditions, as well as in pathology, including cancer and various autoimmune diseases. CCR6-mediated signals are essential for immune responses to microbes in the intestinal mucosa and in the modulation of inflammatory responses initiated by tissue insult and trauma (PubMed:21376174). CCR6 is essential for the recruitment of both the pro-inflammatory IL17 producing helper T-cells (Th17) and the regulatory T-cells (Treg) to sites of inflammation. Required for the normal migration of Th17 cells in Peyers-patches and other related tissue sites of the intestine and plays a role in regulating effector T-cell balance and distribution in inflamed intestine. Plays an important role in the coordination of early thymocyte precursor migration events important for normal subsequent thymocyte precursor development, but is not required for the formation of normal thymic natural regulatory T-cells (nTregs). Required for optimal differentiation of DN2 and DN3 thymocyte precursors. Essential for B-cell localization in the subepithelial dome of Peyers-patches and for efficient B-cell isotype switching to IgA in the Peyers-patches. Essential for appropriate anatomical distribution of memory B-cells in the spleen and for the secondary recall response of memory B-cells (By similarity). Positively regulates sperm motility and chemotaxis via its binding to CCL20 (PubMed:23765988).
Alternative names
CD196, CKRL3, CMKBR6, GPR29, STRL22, CCR6, C-C chemokine receptor type 6, C-C CKR-6, CC-CKR-6, CCR-6, Chemokine receptor-like 3, DRY6, G-protein coupled receptor 29, GPR-CY4, LARC receptor, CKR-L3, GPRCY4
Recommended products
Rabbit Recombinant Monoclonal CCR6 antibody. Carrier free. Suitable for I-ELISA, IP, WB, IHC-P and reacts with Synthetic peptide - Human, Human samples. Cited in 2 publications.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- EPR22259
- Purification technique
- Affinity purification Protein A
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab243852 is the carrier-free version of Anti-CCR6 antibody [EPR22259] ab227036.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
CCR6 also known as CD196 is a chemokine receptor with a molecular mass of approximately 44 kDa. This protein is primarily expressed on the surface of various immune cells including T cells B cells and dendritic cells. Through its interaction with its only known ligand CCL20 also known as MIP-3α CCR6 mediates chemotaxis which guides the targeted migration of cells to sites of inflammation or injury. In murine models (mouse) CCR6 expression helps researchers examine immune responses in vivo and its presence is detected using recombinant proteins or antibodies specific for CCR6 such as 'CCR6 antibodies' with techniques including flow cytometry utilizing labels like CCR6-APC or CCR6-fluorochrome.
- Biological function summary
CCR6 plays an essential role in the regulation of immune responses and maintaining mucosal immunity. It is involved in the recruitment of Th17 cells and dendritic cells to inflamed tissues which plays a critical role in initiating and sustaining immune responses. While CCR6 operates primarily as a standalone receptor its engagement influences diverse cellular functions linked to immune surveillance and tolerance. This receptor's expression on lymphoid tissue inducer cells indicates its relevance in the formation of secondary lymphoid organs and in dynamic interactions within the immune system.
- Pathways
CCR6 is a significant component in pathways related to immune cell trafficking and inflammatory responses particularly the MAPK/ERK pathway and the JAK/STAT pathway. In these pathways CCR6 often interacts with related proteins such as CCL20 and other chemokine receptors facilitating cellular migration and activation processes. These interactions underpin the migration of regulatory T cells and B cells which modulate inflammation and affect pathogen clearance ensuring a controlled immune response.
- Associated diseases and disorders
CCR6 has links to conditions such as autoimmune diseases with rheumatoid arthritis and inflammatory bowel disease being notable examples. In rheumatoid arthritis the dysregulated expression of CCR6 attracts Th17 cells into synovial tissues promoting inflammation. Meanwhile inflammatory bowel disease shows a similar pattern of CCR6-mediated chemotaxis that encourages Th17 cell recruitment. In these contexts CCR6 cooperates with other immune mediators like CCL20 to perpetuate disease-related inflammatory processes highlighting its potential as a therapeutic target in immune modulation.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
5 product images
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CCR6 antibody [EPR22259] - BSA and Azide free (ab243852), expandable thumbnail](https://content.abcam.com/products/images/ccr6-antibody-epr22259-bsa-and-azide-free-ab243852--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img48166.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CCR6 antibody [EPR22259] - BSA and Azide free (ab243852)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CCR6 antibody [EPR22259] - BSA and Azide free (ab243852)
Immunohistochemical analysis of paraffin-embedded human tonsil tissue labeling CCR6 with Anti-CCR6 antibody [EPR22259] ab227036 at 1/1000 dilution, followed by the ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Membranous staining on human tonsil (PMID: 12514792) is observed. Counterstained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is the ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
The section was incubated with Anti-CCR6 antibody [EPR22259] ab227036 for 30 mins at 37°C. The immunostaining was performed on a Leica Biosystems BOND® RX instrument.
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-CCR6 antibody [EPR22259] ab227036).
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CCR6 antibody [EPR22259] - BSA and Azide free (ab243852), expandable thumbnail](https://content.abcam.com/products/images/ccr6-antibody-epr22259-bsa-and-azide-free-ab243852--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img81213.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CCR6 antibody [EPR22259] - BSA and Azide free (ab243852)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CCR6 antibody [EPR22259] - BSA and Azide free (ab243852)
Immunohistochemical analysis of paraffin-embedded human Hodgkin's lymphoma tissue labeling CCR6 with Anti-CCR6 antibody [EPR22259] ab227036 at 1/1000 dilution, followed by the ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). Membranous staining on human Hodgkin's lymphoma (PMID: 12514792) is observed. Counterstained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is the ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.
The section was incubated with Anti-CCR6 antibody [EPR22259] ab227036 for 30 mins at 37°C. The immunostaining was performed on a Leica Biosystems BOND® RX instrument.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-CCR6 antibody [EPR22259] ab227036).
![Immunoprecipitation - Anti-CCR6 antibody [EPR22259] - BSA and Azide free (ab243852), expandable thumbnail](https://content.abcam.com/products/images/ccr6-antibody-epr22259-bsa-and-azide-free-ab243852--immunoprecipitation-img11835.jpg "Immunoprecipitation - Anti-CCR6 antibody [EPR22259] - BSA and Azide free (ab243852)")
Immunoprecipitation - Anti-CCR6 antibody [EPR22259] - BSA and Azide free (ab243852)
CCR6 was immunoprecipitated from 0.35 mg HL-60 (Human acute promyelocytic leukemia promyeloblast) whole cell lysate with Anti-CCR6 antibody [EPR22259] ab227036 at 1/30 dilution. Western blot was performed on the immunoprecipitate using Anti-CCR6 antibody [EPR22259] ab227036 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) was used for detection at 1/1000 dilution.
Lane 1: HL-60 whole cell lysate 10 μg (input).
Lane 2: Anti-CCR6 antibody [EPR22259] ab227036 IP in HL-60 whole cell lysate.
Lane 3: Rabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-CCR6 antibody [EPR22259] ab227036 in HL-60 whole cell lysate.Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 1 second.This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-CCR6 antibody [EPR22259] ab227036).
All lanes: Immunoprecipitation - Anti-CCR6 antibody [EPR22259] (Anti-CCR6 antibody [EPR22259] ab227036)
Predicted band size: 42 kDa
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CCR6 antibody [EPR22259] - BSA and Azide free (ab243852), expandable thumbnail](https://content.abcam.com/products/images/ccr6-antibody-epr22259-bsa-and-azide-free-ab243852--immunohistochemistry-formalin-pfa-fixed-paraffin-embedded-sections-img423091.jpg "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CCR6 antibody [EPR22259] - BSA and Azide free (ab243852)")
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CCR6 antibody [EPR22259] - BSA and Azide free (ab243852)
Tissue Microarrays stained for "Anti-CCR6 antibody [EPR22259]" using "Anti-CCR6 antibody [EPR22259] ab227036"in immunohistochemical analysis. This table provides a detailed overview of positive (tick mark) and negative (cross mark) staining per sample type tested. The sections were pre-treated using Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0) for 20 minutes. The sections were incubated with Anti-CCR6 antibody [EPR22259] ab227036 for 30 mins at 37? followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (Rabbit specific IHC polymer detection kit HRP/DAB ab209101). The immunostaining was performed on a Leica Biosystems BOND® RX instrument.
![Indirect ELISA - Anti-CCR6 antibody [EPR22259] - BSA and Azide free (ab243852), expandable thumbnail](https://content.abcam.com/products/images/ccr6-antibody-epr22259-bsa-and-azide-free-ab243852--indirect-elisa-img417708.jpg "Indirect ELISA - Anti-CCR6 antibody [EPR22259] - BSA and Azide free (ab243852)")
Indirect ELISA - Anti-CCR6 antibody [EPR22259] - BSA and Azide free (ab243852)
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (Anti-CCR6 antibody [EPR22259] ab227036).
Indirect ELISA showing primary antibody Anti-CCR6 antibody [EPR22259] ab227036 binding to Human CCR6. Antigen concentration is 1000 ng/ml.
Binding of Anti-CCR6 antibody [EPR22259] ab227036 was assessed in a serial dilution range 1000-0 ng/ml.
Binding was detected using the secondary antibody, Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at 1/2500 dilution.
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