Anti-CCR6 antibody [EPR22259] - BSA and Azide free

• IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CCR6 antibody [EPR22259] - BSA and Azide free (AB243852)

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227036).

Tissue Microarrays stained for CCR6 using ab227036 in immunohistochemical analysis. This table provides a detailed overview of positive (tick mark) and negative (cross mark) staining per sample type tested. The sections were pre-treated using Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0) for 20 minutes. The sections were incubated with ab227036 for 30 mins at 37°C followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). The immunostaining was performed on a Leica Biosystems BOND® RX instrument.

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CCR6 antibody [EPR22259] - BSA and Azide free (AB243852)

Immunohistochemical analysis of paraffin-embedded human Hodgkin's lymphoma tissue labeling CCR6 with ab227036 at 1/1000 dilution, followed by the ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Membranous staining on human Hodgkin's lymphoma (PMID : 12514792) is observed. Counterstained with hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is the ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

The section was incubated with ab227036 for 30 mins at 37°C. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227036).

• IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CCR6 antibody [EPR22259] - BSA and Azide free (AB243852)

Immunohistochemical analysis of paraffin-embedded human tonsil tissue labeling CCR6 with ab227036 at 1/1000 dilution, followed by the ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Membranous staining on human tonsil (PMID : 12514792) is observed. Counterstained with hematoxylin.

Secondary antibody only control : Used PBS instead of primary antibody, secondary antibody is the ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

The section was incubated with ab227036 for 30 mins at 37°C. The immunostaining was performed on a Leica Biosystems BOND^® RX instrument.

Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227036).

• IP

Supplier Data

Immunoprecipitation - Anti-CCR6 antibody [EPR22259] - BSA and Azide free (AB243852)

CCR6 was immunoprecipitated from 0.35 mg HL-60 (Human acute promyelocytic leukemia promyeloblast) whole cell lysate with ab227036 at 1/30 dilution. Western blot was performed on the immunoprecipitate using ab227036 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366) was used for detection at 1/1000 dilution.

Lane 1 : HL-60 whole cell lysate 10 μg (input).
Lane 2 : ab227036 IP in HL-60 whole cell lysate.
Lane 3 : Rabbit monoclonal IgG (ab172730) instead of ab227036 in HL-60 whole cell lysate.

Blocking and dilution buffer and concentration : 5% NFDM/TBST.
Exposure time : 1 second.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227036).

All lanes:

Immunoprecipitation - Anti-CCR6 antibody [EPR22259] (<a href='/en-us/products/primary-antibodies/ccr6-antibody-epr22259-ab227036'>ab227036</a>)

Predicted band size: 42 kDa

false

• I-ELISA

Lab

Indirect ELISA - Anti-CCR6 antibody [EPR22259] - BSA and Azide free (AB243852)

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab227036). Indirect ELISA showing primary antibody ab227036 binding to Human CCR6. Antigen concentration is 1000 ng/ml. Binding of ab227036 was assessed in a serial dilution range 1000-0 ng/ml. Binding was detected using the secondary antibody, Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at 1/2500 dilution.