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AB303672

Anti-CCR6 antibody [EPR24085-145]

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Rabbit Recombinant Monoclonal CCR6 antibody. Suitable for ICC/IF, Flow Cyt and reacts with Human samples.

View Alternative Names

CD196, CKRL3, CMKBR6, GPR29, STRL22, CCR6, C-C chemokine receptor type 6, C-C CKR-6, CC-CKR-6, CCR-6, Chemokine receptor-like 3, DRY6, G-protein coupled receptor 29, GPR-CY4, LARC receptor, CKR-L3, GPRCY4

3 Images
Immunocytochemistry/ Immunofluorescence - Anti-CCR6 antibody [EPR24085-145] (AB303672)
  • ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-CCR6 antibody [EPR24085-145] (AB303672)

Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized Human PBMC (human primary peripheral blood mononuclear cell) cells labelling CCR6 with ab303672 at 1/50 (10.68 ug/ml) dilution, followed by ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed antibody at 1/1000 2ug/ml dilution (Green). Confocal image showing cytoplasmic staining in subsets of human PBMCs. Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8). is observed. ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Red). The Nuclear counterstain was DAPI (Blue). Secondary antibody only control : Secondary antibody is ab150081 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 2ug/ml dilution.

Flow Cytometry - Anti-CCR6 antibody [EPR24085-145] (AB303672)
  • Flow Cyt

Supplier Data

Flow Cytometry - Anti-CCR6 antibody [EPR24085-145] (AB303672)

Flow cytometric analysis of / fixed / permeabilized Human peripheral blood mononuclear cell (PBMC) cells labelling CCR6 with ab303672 at 1/500 dilution (0.1ug)/ Right (Red) compared with a Rabbit monoclonal IgG (ab172730) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/2000 dilution was used as the secondary antibody. Cells were stained with rabbit IgG or ab303672. Then stained with anti-CD14 conjugated to BV510.Gated on viable cells.

Flow Cytometry - Anti-CCR6 antibody [EPR24085-145] (AB303672)
  • Flow Cyt

Supplier Data

Flow Cytometry - Anti-CCR6 antibody [EPR24085-145] (AB303672)

Flow cytometric analysis of / fixed / permeabilized Human peripheral blood mononuclear cell (PBMC) cells labelling CCR6 with ab303672 at 1/500 dilution (0.1ug)/ Right (Red) compared with a Rabbit monoclonal IgG (ab172730) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/2000 dilution was used as the secondary antibody. Cells were stained with rabbit IgG or ab303672. Then stained with anti-CD19 conjugated to Alexa Fluor® 647.Gated on viable lymphocytes.

  • Carrier free

    Anti-CCR6 antibody [EPR24085-145] - BSA and Azide free

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR24085-145

Isotype

IgG

Carrier free

No

Reacts with

Human

Applications

Flow Cyt, ICC/IF

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

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Product details

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CCR6 also known as CD196 is a chemokine receptor with a molecular mass of approximately 44 kDa. This protein is primarily expressed on the surface of various immune cells including T cells B cells and dendritic cells. Through its interaction with its only known ligand CCL20 also known as MIP-3α CCR6 mediates chemotaxis which guides the targeted migration of cells to sites of inflammation or injury. In murine models (mouse) CCR6 expression helps researchers examine immune responses in vivo and its presence is detected using recombinant proteins or antibodies specific for CCR6 such as 'CCR6 antibodies' with techniques including flow cytometry utilizing labels like CCR6-APC or CCR6-fluorochrome.
Biological function summary

CCR6 plays an essential role in the regulation of immune responses and maintaining mucosal immunity. It is involved in the recruitment of Th17 cells and dendritic cells to inflamed tissues which plays a critical role in initiating and sustaining immune responses. While CCR6 operates primarily as a standalone receptor its engagement influences diverse cellular functions linked to immune surveillance and tolerance. This receptor's expression on lymphoid tissue inducer cells indicates its relevance in the formation of secondary lymphoid organs and in dynamic interactions within the immune system.

Pathways

CCR6 is a significant component in pathways related to immune cell trafficking and inflammatory responses particularly the MAPK/ERK pathway and the JAK/STAT pathway. In these pathways CCR6 often interacts with related proteins such as CCL20 and other chemokine receptors facilitating cellular migration and activation processes. These interactions underpin the migration of regulatory T cells and B cells which modulate inflammation and affect pathogen clearance ensuring a controlled immune response.

CCR6 has links to conditions such as autoimmune diseases with rheumatoid arthritis and inflammatory bowel disease being notable examples. In rheumatoid arthritis the dysregulated expression of CCR6 attracts Th17 cells into synovial tissues promoting inflammation. Meanwhile inflammatory bowel disease shows a similar pattern of CCR6-mediated chemotaxis that encourages Th17 cell recruitment. In these contexts CCR6 cooperates with other immune mediators like CCL20 to perpetuate disease-related inflammatory processes highlighting its potential as a therapeutic target in immune modulation.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Receptor for the C-C type chemokine CCL20 (PubMed : 9169459). Binds to CCL20 and subsequently transduces a signal by increasing the intracellular calcium ion levels (PubMed : 20068036). Although CCL20 is its major ligand it can also act as a receptor for non-chemokine ligands such as beta-defensins (PubMed : 25585877). Binds to defensin DEFB1 leading to increase in intracellular calcium ions and cAMP levels. Its binding to DEFB1 is essential for the function of DEFB1 in regulating sperm motility and bactericidal activity (PubMed : 25122636). Binds to defensins DEFB4 and DEFB4A/B and mediates their chemotactic effects (PubMed : 20068036). The ligand-receptor pair CCL20-CCR6 is responsible for the chemotaxis of dendritic cells (DC), effector/ memory T-cells and B-cells and plays an important role at skin and mucosal surfaces under homeostatic and inflammatory conditions, as well as in pathology, including cancer and various autoimmune diseases. CCR6-mediated signals are essential for immune responses to microbes in the intestinal mucosa and in the modulation of inflammatory responses initiated by tissue insult and trauma (PubMed : 21376174). CCR6 is essential for the recruitment of both the pro-inflammatory IL17 producing helper T-cells (Th17) and the regulatory T-cells (Treg) to sites of inflammation. Required for the normal migration of Th17 cells in Peyers-patches and other related tissue sites of the intestine and plays a role in regulating effector T-cell balance and distribution in inflamed intestine. Plays an important role in the coordination of early thymocyte precursor migration events important for normal subsequent thymocyte precursor development, but is not required for the formation of normal thymic natural regulatory T-cells (nTregs). Required for optimal differentiation of DN2 and DN3 thymocyte precursors. Essential for B-cell localization in the subepithelial dome of Peyers-patches and for efficient B-cell isotype switching to IgA in the Peyers-patches. Essential for appropriate anatomical distribution of memory B-cells in the spleen and for the secondary recall response of memory B-cells (By similarity). Positively regulates sperm motility and chemotaxis via its binding to CCL20 (PubMed : 23765988).
See full target information CCR6
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Product promise

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