AB93086

Anti-CCR6 antibody [MM0066-3L1]

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(3 Publications)

Mouse Monoclonal CCR6 antibody. Suitable for Flow Cyt, IHC-P and reacts with Human samples. Cited in 3 publications. Immunogen corresponding to Cell preparation containing CCR6 protein.

View Alternative Names

CD196, CKRL3, CMKBR6, GPR29, STRL22, CCR6, C-C chemokine receptor type 6, C-C CKR-6, CC-CKR-6, CCR-6, Chemokine receptor-like 3, DRY6, G-protein coupled receptor 29, GPR-CY4, LARC receptor, CKR-L3, GPRCY4

3 Images

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CCR6 antibody [MM0066-3L1] (AB93086)

IHC-P

Supplier Data

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CCR6 antibody [MM0066-3L1] (AB93086)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human placental tissue section (4μm) labelling CCR6 with ab93086. Tissue was treated with 15μg/ml Protenase for antigen retrieval and fixed with 4% PFA. A HRP-conjugated anti-mouse IgG was used as the secondary antibody (30 minutes at room temperature) followed by DAB color development.

Immunohistochemistry paraffin embedded sections - Anti-CCR6 antibody [MM0066-3L1] (AB93086)

IHC-P

Unknown

Immunohistochemistry paraffin embedded sections - Anti-CCR6 antibody [MM0066-3L1] (AB93086)

IHC image of ab93086 staining in Human Tonsil formalin fixed paraffin embedded tissue section, performed on a Leica Bond^TM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab93086, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

Flow Cytometry - Anti-CCR6 antibody [MM0066-3L1] (AB93086)

Flow Cyt

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Flow Cytometry - Anti-CCR6 antibody [MM0066-3L1] (AB93086)

Overlay histogram showing Jurkat cells stained with ab93086 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab93086, 2μg/1x10⁶ cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2μg/1x10⁶ cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in Jurkat cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

Key facts

Host species

Mouse

Clonality

Monoclonal

Clone number

MM0066-3L1

Isotype

IgG2b

Carrier free

Reacts with

Human

Applications

Flow Cyt, IHC-P

applications

Immunogen

Cell preparation containing CCR6 protein. The exact immunogen used to generate this antibody is proprietary information.

P51684

Specificity

This antibody detects CCR6, but not other CCRs.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1/100 - 1/800", "FlowCyt-species-notes": "<p><a href='/en-us/products/primary-antibodies/mouse-igg2b-kappa-monoclonal-7e10g10-isotype-control-ab170192'>ab170192</a> - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody.</p>", "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1 µg/mL", "IHCP-species-notes": "<p></p>" } } }

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Properties and storage information

Form

Liquid

Purification technique

Affinity purification Protein G

Purification notes

The IgG fraction of culture supernatant was purified by Protein G affinity chromatography and 0.2 µm filtered.

Storage buffer

Constituents: PBS

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CCR6 also known as CD196 is a chemokine receptor with a molecular mass of approximately 44 kDa. This protein is primarily expressed on the surface of various immune cells including T cells B cells and dendritic cells. Through its interaction with its only known ligand CCL20 also known as MIP-3α CCR6 mediates chemotaxis which guides the targeted migration of cells to sites of inflammation or injury. In murine models (mouse) CCR6 expression helps researchers examine immune responses in vivo and its presence is detected using recombinant proteins or antibodies specific for CCR6 such as 'CCR6 antibodies' with techniques including flow cytometry utilizing labels like CCR6-APC or CCR6-fluorochrome.

Biological function summary

CCR6 plays an essential role in the regulation of immune responses and maintaining mucosal immunity. It is involved in the recruitment of Th17 cells and dendritic cells to inflamed tissues which plays a critical role in initiating and sustaining immune responses. While CCR6 operates primarily as a standalone receptor its engagement influences diverse cellular functions linked to immune surveillance and tolerance. This receptor's expression on lymphoid tissue inducer cells indicates its relevance in the formation of secondary lymphoid organs and in dynamic interactions within the immune system.

Pathways

CCR6 is a significant component in pathways related to immune cell trafficking and inflammatory responses particularly the MAPK/ERK pathway and the JAK/STAT pathway. In these pathways CCR6 often interacts with related proteins such as CCL20 and other chemokine receptors facilitating cellular migration and activation processes. These interactions underpin the migration of regulatory T cells and B cells which modulate inflammation and affect pathogen clearance ensuring a controlled immune response.

CCR6 has links to conditions such as autoimmune diseases with rheumatoid arthritis and inflammatory bowel disease being notable examples. In rheumatoid arthritis the dysregulated expression of CCR6 attracts Th17 cells into synovial tissues promoting inflammation. Meanwhile inflammatory bowel disease shows a similar pattern of CCR6-mediated chemotaxis that encourages Th17 cell recruitment. In these contexts CCR6 cooperates with other immune mediators like CCL20 to perpetuate disease-related inflammatory processes highlighting its potential as a therapeutic target in immune modulation.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

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Target data

Receptor for the C-C type chemokine CCL20 (PubMed : 9169459). Binds to CCL20 and subsequently transduces a signal by increasing the intracellular calcium ion levels (PubMed : 20068036). Although CCL20 is its major ligand it can also act as a receptor for non-chemokine ligands such as beta-defensins (PubMed : 25585877). Binds to defensin DEFB1 leading to increase in intracellular calcium ions and cAMP levels. Its binding to DEFB1 is essential for the function of DEFB1 in regulating sperm motility and bactericidal activity (PubMed : 25122636). Binds to defensins DEFB4 and DEFB4A/B and mediates their chemotactic effects (PubMed : 20068036). The ligand-receptor pair CCL20-CCR6 is responsible for the chemotaxis of dendritic cells (DC), effector/ memory T-cells and B-cells and plays an important role at skin and mucosal surfaces under homeostatic and inflammatory conditions, as well as in pathology, including cancer and various autoimmune diseases. CCR6-mediated signals are essential for immune responses to microbes in the intestinal mucosa and in the modulation of inflammatory responses initiated by tissue insult and trauma (PubMed : 21376174). CCR6 is essential for the recruitment of both the pro-inflammatory IL17 producing helper T-cells (Th17) and the regulatory T-cells (Treg) to sites of inflammation. Required for the normal migration of Th17 cells in Peyers-patches and other related tissue sites of the intestine and plays a role in regulating effector T-cell balance and distribution in inflamed intestine. Plays an important role in the coordination of early thymocyte precursor migration events important for normal subsequent thymocyte precursor development, but is not required for the formation of normal thymic natural regulatory T-cells (nTregs). Required for optimal differentiation of DN2 and DN3 thymocyte precursors. Essential for B-cell localization in the subepithelial dome of Peyers-patches and for efficient B-cell isotype switching to IgA in the Peyers-patches. Essential for appropriate anatomical distribution of memory B-cells in the spleen and for the secondary recall response of memory B-cells (By similarity). Positively regulates sperm motility and chemotaxis via its binding to CCL20 (PubMed : 23765988).

See full target information CCR6

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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Biology of reproduction 101:138-147 PubMed30985884

2019

Impaired decidualization caused by downregulation of circadian clock gene BMAL1 contributes to human recurrent miscarriage†.

Applications

Unspecified application

Species

Unspecified reactive species

Shijian Lv,Na Wang,Jin Ma,Wei-Ping Li,Zi-Jiang Chen,Cong Zhang

PloS one 11:e0157740 PubMed27336468

2016

Identification of the Single Immunodominant Region of the Native Human CC Chemokine Receptor 6 Recognized by Mouse Monoclonal Antibodies.

Applications

Unspecified application

Species

Unspecified reactive species

Karim Dorgham,Cécile Dejou,Christophe Piesse,Guy Gorochov,Jérôme Pène,Hans Yssel

BioMed research international 2015:617143 PubMed26634210

2015

Expression and Prognostic Significance of Macrophage Inflammatory Protein-3 Alpha and Cystatin A in Nasopharyngeal Carcinoma.

Applications

IHC

Species

Unspecified reactive species

Minzhong Tang,Ningjiang Ou,Cheng Li,Aiying Lu,Jun Li,Liping Ma,Weiming Zhong,Jianquan Gao,Yuming Zheng,Yonglin Cai

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

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Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-CCR6 antibody [MM0066-3L1]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CCR6 antibody [MM0066-3L1] (AB93086)

Immunohistochemistry paraffin embedded sections - Anti-CCR6 antibody [MM0066-3L1] (AB93086)

Flow Cytometry - Anti-CCR6 antibody [MM0066-3L1] (AB93086)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Specificity

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Reactivity data

Properties and storage information

Form

Purification technique

Purification notes

Storage buffer

Shipped at conditions

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (3)

Product promise