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AB52602

Anti-CCR7 antibody [4B12]

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(3 Publications)

Rat Monoclonal CCR7 antibody. Suitable for Flow Cyt and reacts with Mouse samples. Cited in 3 publications.

View Alternative Names

CD197, Cmkbr7, Ebi1, Ebi1h, Ccr7, C-C chemokine receptor type 7, C-C CKR-7, CC-CKR-7, CCR-7, Epstein-Barr virus-induced G-protein coupled receptor 1, MIP-3 beta receptor, EBI1, EBV-induced G-protein coupled receptor 1

1 Images
Flow Cytometry - Anti-CCR7 antibody [4B12] (AB52602)
  • Flow Cyt

Supplier Data

Flow Cytometry - Anti-CCR7 antibody [4B12] (AB52602)

C57BL6 mouse splenocytes were incubated with Rat IgG2b, kappa monoclonal [RTK4530] (ab18541) (left) or anti-CCR7 [4B12] antibody (ab52602) (right) at 10ug/ml for 30 min at 4+°C. The secondary antibody Goat Anti-Rat IgG H&L (Alexa Fluor® 488) (ab150157) was used at 1/2000 dilution for 30 min at 4+°C. The cells were simultaneously stained with anti-CD3ε PerCP (10ug/ml) (ab190299).

Acquisition of >30,000 total events were collected. Gating strategy - live lymphocytes

Key facts

Host species

Rat

Clonality

Monoclonal

Clone number

4B12

Isotype

IgG2a

Light chain type

kappa

Carrier free

No

Reacts with

Mouse

Applications

Flow Cyt

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Mouse": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "5-10 µg/mL", "FlowCyt-species-notes": "<p><a href='/en-us/products/primary-antibodies/rat-igg2b-kappa-monoclonal-rtk4530-isotype-control-ab18541'>ab18541</a> - Rat IgG2b monoclonal, is suitable for use as an isotype control with this antibody.</p>" } } }
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Product details

Want a custom formulation?
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein G
Storage buffer
Preservative: 0.02% Sodium azide Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CCR7 also known as CD197 is a chemokine receptor with a molecular mass of approximately 43-48 kDa. The receptor is known to be expressed predominantly in lymphoid tissues such as the spleen and lymph nodes. Additionally CCR7 is found on various immune cells like T cells and dendritic cells. Researchers study CCR7 using different methods such as CCR7-PE and CCR7-APC antibody staining in flow cytometry to examine its expression on immune cells in various conditions.
Biological function summary

CCR7 plays an important role in directing the migration and homeostasis of T cells and dendritic cells. It is not part of a larger protein complex but interacts functionally with specific signaling enzymes upon activation. CCR7 is important for the function of the immune system aiding in the development of adaptive immunity by controlling lymphocyte movement and positioning in lymphoid tissues. This receptor assists in guiding cells to specific lymphoid organs where immune responses are initiated and maintained.

Pathways

CCR7 is significantly involved in the CCL19/CCL21 chemokine signaling pathways which help direct immune cell positioning. CCR7 interacts with these chemokines to regulate the trafficking of cells to lymphoid tissues. Additionally it works in tandem with other receptors like CCR5 in the regulation of immune cell mobilization. These pathways are essential in coordinating the immune response during infection and inflammation.

CCR7 is connected with cancer metastasis and chronic inflammatory diseases. Its role in cell migration makes it relevant in the metastasis of certain cancers where cells misregulate their movement and spread from the original tumor site. Additionally CCR7's involvement in chronic inflammatory diseases highlights its connection to rheumatoid arthritis where it may interact with other proteins such as CCL19 and CCL21 contributing to disease progression. Understanding these interactions helps develop potential therapeutic strategies targeting CCR7 in such diseases.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Receptor for the MIP-3-beta chemokine.
See full target information Ccr7
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Publications (3)

Recent publications for all applications. Explore the full list and refine your search

Bioactive materials 21:520-530 PubMed36185735

2022

Puerarin@Chitosan composite for infected bone repair through mimicking the bio-functions of antimicrobial peptides.

Applications

Unspecified application

Species

Unspecified reactive species

Liping Ouyang,Baohui Chen,Xingdan Liu,Donghui Wang,Yang Li,Yun Liao,Kelvin W K Yeung,Xuanyong Liu

Immunity 21:279-88 PubMed15308107

2004

CCR7 governs skin dendritic cell migration under inflammatory and steady-state conditions.

Applications

Unspecified application

Species

Unspecified reactive species

Lars Ohl,Mariette Mohaupt,Niklas Czeloth,Gabriele Hintzen,Ziba Kiafard,Jörg Zwirner,Thomas Blankenstein,Golo Henning,Reinhold Förster

Journal of leukocyte biology 76:472-6 PubMed15197229

2004

Analysis of the CCR7 expression on murine bone marrow-derived and spleen dendritic cells.

Applications

Unspecified application

Species

Unspecified reactive species

Uwe Ritter,Florian Wiede,Dirk Mielenz,Ziba Kiafard,Jörg Zwirner,Heinrich Körner
View all publications
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