Rat Monoclonal CCR7 antibody. Carrier free. Suitable for Flow Cyt and reacts with Mouse samples.
Images
![Flow Cytometry - Anti-CCR7 antibody [4B12] - BSA and Azide free (AB269572), expandable thumbnail](https://content.abcam.com/products/images/ccr7-antibody-4b12-bsa-and-azide-free-ab269572--flow-cytometry-img42283.jpg/_jcr_content/renditions/webp-475.webp "Flow Cytometry - Anti-CCR7 antibody [4B12] - BSA and Azide free (AB269572)")
Key facts
- Isotype
IgG2a
- Host species
Rat
- Storage buffer
Constituents: PBS
- Form
Liquid
- Clonality
Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| Flow Cyt | |
|---|---|
| Mouse | Tested |
Flow Cyt
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Mouse | Dilution info 5.00000-10.00000 µg/mL | Notes Rat IgG2b, kappa monoclonal [RTK4530] - Isotype control ab18541 - Rat IgG2b monoclonal, is suitable for use as an isotype control with this antibody. |
Alternative names
Cmkbr7, Ebi1, Ebi1h, Ebi1, Cmkbr7, Ccr7, Ebi1h, C-C chemokine receptor type 7, C-C CKR-7, CC-CKR-7, CCR-7, Epstein-Barr virus-induced G-protein coupled receptor 1, MIP-3 beta receptor, EBI1, EBV-induced G-protein coupled receptor 1
Recommended products
Rat Monoclonal CCR7 antibody. Carrier free. Suitable for Flow Cyt and reacts with Mouse samples.
Key facts
- Isotype
IgG2a
- Host species
Rat
- Storage buffer
Constituents: PBS
- Form
Liquid
- Clonality
Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
Yes
- Clone number
4B12
- Purification technique
Affinity purification Protein G
- Light chain type
kappa
- Concentration
Storage
- Shipped at conditions
Blue Ice
- Appropriate short-term storage conditions
+4°C
- Appropriate long-term storage conditions
+4°C
- Storage information
Do Not Freeze
Notes
ab269572 is the carrier-free version of Anti-CCR7 antibody [4B12] ab52602.
Abcam is leading the way to address reproducibility in scientific research with our highly validated recombinant monoclonal and recombinant multiclonal antibodies. Search & select one of Abcam's thousands of recombinant alternatives to eliminate batch-variability and unnecessary animal use.
If you do not find a host species to meet your needs, our catalogue and custom Chimeric range provides scientists the specificity of Abcam's RabMAbs in the species backbone of your choice. Remember to also review our range of edited cell lines, proteins and biochemicals relevant to your target that may help you further your research goals.
Abcam antibodies are extensively validated in a wide range of species and applications, so please check the reagent specifications meet your scientific needs before purchasing. If you have any questions or bespoke requirements, simply visit the Contact Us page to send us an inquiry or contact our Support Team ahead of purchase.
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
CCR7 also known as CD197 is a chemokine receptor with a molecular mass of approximately 43-48 kDa. The receptor is known to be expressed predominantly in lymphoid tissues such as the spleen and lymph nodes. Additionally CCR7 is found on various immune cells like T cells and dendritic cells. Researchers study CCR7 using different methods such as CCR7-PE and CCR7-APC antibody staining in flow cytometry to examine its expression on immune cells in various conditions.
- Biological function summary
CCR7 plays an important role in directing the migration and homeostasis of T cells and dendritic cells. It is not part of a larger protein complex but interacts functionally with specific signaling enzymes upon activation. CCR7 is important for the function of the immune system aiding in the development of adaptive immunity by controlling lymphocyte movement and positioning in lymphoid tissues. This receptor assists in guiding cells to specific lymphoid organs where immune responses are initiated and maintained.
- Pathways
CCR7 is significantly involved in the CCL19/CCL21 chemokine signaling pathways which help direct immune cell positioning. CCR7 interacts with these chemokines to regulate the trafficking of cells to lymphoid tissues. Additionally it works in tandem with other receptors like CCR5 in the regulation of immune cell mobilization. These pathways are essential in coordinating the immune response during infection and inflammation.
- Associated diseases and disorders
CCR7 is connected with cancer metastasis and chronic inflammatory diseases. Its role in cell migration makes it relevant in the metastasis of certain cancers where cells misregulate their movement and spread from the original tumor site. Additionally CCR7's involvement in chronic inflammatory diseases highlights its connection to rheumatoid arthritis where it may interact with other proteins such as CCL19 and CCL21 contributing to disease progression. Understanding these interactions helps develop potential therapeutic strategies targeting CCR7 in such diseases.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
1 product image
![Flow Cytometry - Anti-CCR7 antibody [4B12] - BSA and Azide free (ab269572), expandable thumbnail](https://content.abcam.com/products/images/ccr7-antibody-4b12-bsa-and-azide-free-ab269572--flow-cytometry-img42283.jpg "Flow Cytometry - Anti-CCR7 antibody [4B12] - BSA and Azide free (ab269572)")
Flow Cytometry - Anti-CCR7 antibody [4B12] - BSA and Azide free (ab269572)
C57BL6 mouse splenocytes were incubated with Rat IgG2b, kappa monoclonal [RTK4530] (Rat IgG2b, kappa monoclonal [RTK4530] - Isotype control ab18541) (left) or anti-CCR7 [4B12] antibody (Anti-CCR7 antibody [4B12] ab52602) (right) at 10ug/ml for 30 min at 4+°C. The secondary antibody Goat Anti-Rat IgG H&L (Alexa Fluor® 488) (Goat Anti-Rat IgG H&L (Alexa Fluor® 488) ab150157) was used at 1/2000 dilution for 30 min at 4+°C. The cells were simultaneously stained with anti-CD3ε PerCP (10ug/ml) (PerCP Anti-CD3 epsilon antibody [145-2C11] ab190299).
Acquisition of >30,000 total events were collected. Gating strategy - live lymphocytes
This data was developed using the same antibody clone in a different buffer formulation containing PBS and sodium azide (Anti-CCR7 antibody [4B12] ab52602).
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