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AB185745

Anti-CCR7 antibody [4B12] - Low endotoxin, Azide free

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(1 Publication)

Rat Monoclonal CCR7 antibody. Carrier free. Suitable for Flow Cyt and reacts with Rat samples. Cited in 1 publication. Immunogen corresponding to Cell preparation containing Ccr7 protein.

View Alternative Names

CD197, Cmkbr7, Ebi1, Ebi1h, Ccr7, C-C chemokine receptor type 7, C-C CKR-7, CC-CKR-7, CCR-7, Epstein-Barr virus-induced G-protein coupled receptor 1, MIP-3 beta receptor, EBI1, EBV-induced G-protein coupled receptor 1

1 Images
Flow Cytometry - Anti-CCR7 antibody [4B12] - Low endotoxin, Azide free (AB185745)
  • Flow Cyt

Supplier Data

Flow Cytometry - Anti-CCR7 antibody [4B12] - Low endotoxin, Azide free (AB185745)

Flow cytometry analysis of rat RBL cells co-expressing mouse CCR7 and a GFP protein from an IRES-containing retrovirus. These cells were stained with either rat IgG2a kappa Isotype Control PE (left) or 1 μg of ab185745 (right) followed by F(ab')2 anti-rat IgG PE. GFP expression is shown in FL1 on the X-axis. Total viable cells were used for analysis.

  • Unconjugated

    Anti-CCR7 antibody [4B12]

  • 660 APC

    APC Anti-CCR7 antibody [4B12]

  • 578 PE

    PE Anti-CCR7 antibody [4B12]

  • 578 PE

    PE Anti-CCR7 antibody [4B12]

  • 387 mFluor™ UV375

    mFluor™ UV375 Anti-CD197 antibody [4B12]

  • 454 mFluor™ Violet 450

    mFluor™ Violet 450 Anti-CD197 antibody [4B12]

Key facts

Host species

Rat

Clonality

Monoclonal

Clone number

4B12

Isotype

IgG2a

Light chain type

kappa

Carrier free

Yes

Reacts with

Rat

Applications

Flow Cyt

applications

Immunogen

Cell preparation containing Ccr7 protein. The exact immunogen used to generate this antibody is proprietary information.

P47774

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Rat": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1 µg for 10^5-10^8 Cells", "FlowCyt-species-notes": "<p><a href='/en-us/products/primary-antibodies/rat-igg2a-kappa-monoclonal-rtk2758-isotype-control-ab18450'>ab18450</a> - Rat monoclonal IgG2a, is suitable for use as an isotype control with this antibody.</p>" } } }
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Properties and storage information

Form
Liquid
Purification technique
Affinity purification
Purification notes
Endotoxin Level: Less than 0.001 ng/µg antibody, as determined by the LAL assay.
Storage buffer
pH: 7.2
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CCR7 also known as CD197 is a chemokine receptor with a molecular mass of approximately 43-48 kDa. The receptor is known to be expressed predominantly in lymphoid tissues such as the spleen and lymph nodes. Additionally CCR7 is found on various immune cells like T cells and dendritic cells. Researchers study CCR7 using different methods such as CCR7-PE and CCR7-APC antibody staining in flow cytometry to examine its expression on immune cells in various conditions.
Biological function summary

CCR7 plays an important role in directing the migration and homeostasis of T cells and dendritic cells. It is not part of a larger protein complex but interacts functionally with specific signaling enzymes upon activation. CCR7 is important for the function of the immune system aiding in the development of adaptive immunity by controlling lymphocyte movement and positioning in lymphoid tissues. This receptor assists in guiding cells to specific lymphoid organs where immune responses are initiated and maintained.

Pathways

CCR7 is significantly involved in the CCL19/CCL21 chemokine signaling pathways which help direct immune cell positioning. CCR7 interacts with these chemokines to regulate the trafficking of cells to lymphoid tissues. Additionally it works in tandem with other receptors like CCR5 in the regulation of immune cell mobilization. These pathways are essential in coordinating the immune response during infection and inflammation.

CCR7 is connected with cancer metastasis and chronic inflammatory diseases. Its role in cell migration makes it relevant in the metastasis of certain cancers where cells misregulate their movement and spread from the original tumor site. Additionally CCR7's involvement in chronic inflammatory diseases highlights its connection to rheumatoid arthritis where it may interact with other proteins such as CCL19 and CCL21 contributing to disease progression. Understanding these interactions helps develop potential therapeutic strategies targeting CCR7 in such diseases.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Receptor for the MIP-3-beta chemokine.
See full target information Ccr7
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Oral diseases 29:3493-3502 PubMed36251469

2022

Mesenchymal β-catenin signaling affects palatogenesis by regulating α-actinin-4 and F-actin.

Applications

Unspecified application

Species

Unspecified reactive species

Xiaoming Wang,Weilong Liu,Xiao Luo,Qian Zheng,Bing Shi,Renkai Liu,Chenghao Li
View all publications
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Product promise

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