Rabbit Recombinant Monoclonal CCR7 antibody. Carrier free. Suitable for I-ELISA, WB and reacts with Recombinant fragment - Human, Human, Mouse, Rat samples.
Images
![Western blot - Anti-CCR7 antibody [E75] - BSA and Azide free (AB247223), expandable thumbnail](https://content.abcam.com/products/images/ccr7-antibody-e75-bsa-and-azide-free-ab247223--western-blot-img140863.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-CCR7 antibody [E75] - BSA and Azide free (AB247223)")
![ELISA - Anti-CCR7 antibody [E75] - BSA and Azide free (AB247223), expandable thumbnail](https://content.abcam.com/products/images/ccr7-antibody-e75-bsa-and-azide-free-ab247223--elisa-img140081.jpg/_jcr_content/renditions/webp-475.webp "ELISA - Anti-CCR7 antibody [E75] - BSA and Azide free (AB247223)")
![Western blot - Anti-CCR7 antibody [E75] - BSA and Azide free (AB247223), expandable thumbnail](https://content.abcam.com/products/images/ccr7-antibody-e75-bsa-and-azide-free-ab247223--western-blot-img409301.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-CCR7 antibody [E75] - BSA and Azide free (AB247223)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| Flow Cyt | I-ELISA | IHC | IP | ICC/IF | WB | |
|---|---|---|---|---|---|---|
| Human | Not recommended | Expected | Not recommended | Not recommended | Not recommended | Tested |
| Mouse | Not recommended | Expected | Not recommended | Not recommended | Not recommended | Tested |
| Rat | Not recommended | Expected | Not recommended | Not recommended | Not recommended | Tested |
| Cow | Not recommended | Predicted | Not recommended | Not recommended | Not recommended | Predicted |
| Pig | Not recommended | Predicted | Not recommended | Not recommended | Not recommended | Predicted |
| Recombinant fragment - Human | Not recommended | Tested | Not recommended | Not recommended | Not recommended | Not recommended |
Flow Cyt
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
Species Mouse | Dilution info - | Notes ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
Species Rat | Dilution info - | Notes ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
Species Cow | Dilution info - | Notes - |
Species Pig | Dilution info - | Notes - |
Species Recombinant fragment - Human | Dilution info - | Notes - |
I-ELISA
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant fragment - Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info Use at an assay dependent concentration. | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Cow, Pig | Dilution info - | Notes - |
IHC
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat, Cow, Pig, Recombinant fragment - Human | Dilution info - | Notes - |
IP
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat, Cow, Pig, Recombinant fragment - Human | Dilution info - | Notes - |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat, Cow, Pig, Recombinant fragment - Human | Dilution info - | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Mouse, Rat | Dilution info - | Notes - |
PredictedPredicted
| Species | Dilution info | Notes |
|---|---|---|
Species Cow, Pig | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Recombinant fragment - Human | Dilution info - | Notes - |
Target data
Function
Receptor for the MIP-3-beta chemokine. Probable mediator of EBV effects on B-lymphocytes or of normal lymphocyte functions.
Alternative names
CD197, CMKBR7, EBI1, EVI1, CCR7, C-C chemokine receptor type 7, C-C CKR-7, CC-CKR-7, CCR-7, BLR2, CDw197, Epstein-Barr virus-induced G-protein coupled receptor 1, MIP-3 beta receptor, EBV-induced G-protein coupled receptor 1
Recommended products
Rabbit Recombinant Monoclonal CCR7 antibody. Carrier free. Suitable for I-ELISA, WB and reacts with Recombinant fragment - Human, Human, Mouse, Rat samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- E75
- Purification technique
- Affinity purification Protein A
- Specificity
This antibody recognises CCR7/CKR7. It does not cross react with other CKR family members.
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab247223 is the carrier-free version of Anti-CCR7 antibody [E75] ab32075.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
CCR7 also known as CD197 is a chemokine receptor with a molecular mass of approximately 43-48 kDa. The receptor is known to be expressed predominantly in lymphoid tissues such as the spleen and lymph nodes. Additionally CCR7 is found on various immune cells like T cells and dendritic cells. Researchers study CCR7 using different methods such as CCR7-PE and CCR7-APC antibody staining in flow cytometry to examine its expression on immune cells in various conditions.
- Biological function summary
CCR7 plays an important role in directing the migration and homeostasis of T cells and dendritic cells. It is not part of a larger protein complex but interacts functionally with specific signaling enzymes upon activation. CCR7 is important for the function of the immune system aiding in the development of adaptive immunity by controlling lymphocyte movement and positioning in lymphoid tissues. This receptor assists in guiding cells to specific lymphoid organs where immune responses are initiated and maintained.
- Pathways
CCR7 is significantly involved in the CCL19/CCL21 chemokine signaling pathways which help direct immune cell positioning. CCR7 interacts with these chemokines to regulate the trafficking of cells to lymphoid tissues. Additionally it works in tandem with other receptors like CCR5 in the regulation of immune cell mobilization. These pathways are essential in coordinating the immune response during infection and inflammation.
- Associated diseases and disorders
CCR7 is connected with cancer metastasis and chronic inflammatory diseases. Its role in cell migration makes it relevant in the metastasis of certain cancers where cells misregulate their movement and spread from the original tumor site. Additionally CCR7's involvement in chronic inflammatory diseases highlights its connection to rheumatoid arthritis where it may interact with other proteins such as CCL19 and CCL21 contributing to disease progression. Understanding these interactions helps develop potential therapeutic strategies targeting CCR7 in such diseases.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
3 product images
![Western blot - Anti-CCR7 antibody [E75] - BSA and Azide free (ab247223), expandable thumbnail](https://content.abcam.com/products/images/ccr7-antibody-e75-bsa-and-azide-free-ab247223--western-blot-img140863.jpg "Western blot - Anti-CCR7 antibody [E75] - BSA and Azide free (ab247223)")
Western blot - Anti-CCR7 antibody [E75] - BSA and Azide free (ab247223)
This data was developed using Anti-CCR7 antibody [E75] ab32075, the same antibody clone in a different buffer formulation.
The 50kda band in lanes 2-5 human tissue lysates should be IgG heavy chain.
Blocking and dilution buffer: 5% NFDM/TBST.
All lanes: Western blot - Anti-CCR7 antibody [E75] - BSA and Azide free (ab247223) at 1/1000 dilution
Lane 1: K562 (Human chronic myelogenous leukemia lymphoblast) whole cell lysates at 20 µg
Lane 2: Human lung lysate at 20 µg
Lane 3: Human lymph node lysate at 20 µg
Lane 4: Human spleen lysate at 20 µg
Lane 5: Human plasma lysate at 20 µg
Lane 6: Human brain lysate (low/negative expression) at 20 µg
Lane 7: Human liver lysate (low/negative expression) at 20 µg
Lane 8: Mouse lung lysate at 20 µg
Lane 9: Mouse lymph node lysate at 20 µg
Lane 10: Mouse spleen lysate at 20 µg
Lane 11: Mouse plasma lysate at 20 µg
Lane 12: Rat lung lysate at 20 µg
Lane 13: Rat lymph node tissue lysate at 20 µg
SecondaryAll lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/20000 dilution
Predicted band size: 43 kDa
Observed band size: 48 kDa
![ELISA - Anti-CCR7 antibody [E75] - BSA and Azide free (ab247223), expandable thumbnail](https://content.abcam.com/products/images/ccr7-antibody-e75-bsa-and-azide-free-ab247223--elisa-img140081.jpg "ELISA - Anti-CCR7 antibody [E75] - BSA and Azide free (ab247223)")
ELISA - Anti-CCR7 antibody [E75] - BSA and Azide free (ab247223)
This data was developed using Anti-CCR7 antibody [E75] ab32075, the same antibody clone in a different buffer formulation.ELISA analysis of Human CCR7 recombinant protein at 250 ng/mL with Anti-CCR7 antibody [E75] ab32075. An Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) at 1/2500 dilution was used as the secondary antibody.
![Western blot - Anti-CCR7 antibody [E75] - BSA and Azide free (ab247223), expandable thumbnail](https://content.abcam.com/products/images/ccr7-antibody-e75-bsa-and-azide-free-ab247223--western-blot-img409301.jpg "Western blot - Anti-CCR7 antibody [E75] - BSA and Azide free (ab247223)")
Western blot - Anti-CCR7 antibody [E75] - BSA and Azide free (ab247223)
This data was developed using Anti-CCR7 antibody [E75] ab32075, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.All lanes: Western blot - Anti-CCR7 antibody [E75] (Anti-CCR7 antibody [E75] ab32075) at 1/1000 dilution
Lane 1: K-562 (Human chronic myelogenous leukemia lymphoblast) cytoplasm fraction lysate at 20 µg
Lane 2: K-562 (Human chronic myelogenous leukemia lymphoblast) membrane fraction lysate at 20 µg
Lane 3: K-562 (Human chronic myelogenous leukemia lymphoblast) nuclear fraction lysate at 20 µg
Lane 4: HDLM-2 (Human Hodgkin lymphoma) whole cell lysate at 20 µg
Predicted band size: 43 kDa
Observed band size: 48 kDa
Exposure time: 20s
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com