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AB253187

Anti-CCR7 antibody [EPR23192-57]

  • BOND RX™ Validated
  • 20ul selling size
  • RabMAb
  • Recombinant
  • What is this?

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(18 Publications)

Anti-CCR7 antibody [EPR23192-57] (ab253187) is a rabbit monoclonal antibody detecting CCR7 in IHC-P, IHC-Fr. Suitable for Human, Mouse, Rat.

- Biophysical QC for unrivalled batch-batch consistency

View Alternative Names

CD197, CMKBR7, EBI1, EVI1, CCR7, C-C chemokine receptor type 7, C-C CKR-7, CC-CKR-7, CCR-7, BLR2, CDw197, Epstein-Barr virus-induced G-protein coupled receptor 1, MIP-3 beta receptor, EBV-induced G-protein coupled receptor 1

8 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CCR7 antibody [EPR23192-57] (AB253187)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CCR7 antibody [EPR23192-57] (AB253187)

Immunohistochemical analysis of paraffin-embedded Human Hodgkin's lymphoma tissue labeling CCR7 with ab253187 at 1/500 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on RS cells in human Hodgkin's lymphoma. The section was incubated with ab253187 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND RX instrument Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CCR7 antibody [EPR23192-57] (AB253187)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CCR7 antibody [EPR23192-57] (AB253187)

Immunohistochemical analysis of paraffin-embedded Human pancreas tissue labeling CCR7 with ab253187 at 1/500 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Negative control : No staining in human pancreas. The section was incubated with ab253187 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND RX instrument Counterstained with Hematoxylin.

Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CCR7 antibody [EPR23192-57] (AB253187)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CCR7 antibody [EPR23192-57] (AB253187)

Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling CCR7 with ab253187 at 1/500 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining in human tonsil. The section was incubated with ab253187 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND RX instrument Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CCR7 antibody [EPR23192-57] (AB253187)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CCR7 antibody [EPR23192-57] (AB253187)

Immunohistochemical analysis of paraffin-embedded Mouse spleen tissue labeling CCR7 with ab253187 at 1/500 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining in mouse spleen. The section was incubated with ab253187 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins

Immunohistochemistry (Frozen sections) - Anti-CCR7 antibody [EPR23192-57] (AB253187)
  • IHC-Fr

Unknown

Immunohistochemistry (Frozen sections) - Anti-CCR7 antibody [EPR23192-57] (AB253187)

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Rat spleen tissue labeling CCR7 with ab253187 at 1/500 dilution followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 (2 ug/ml) dilution (Green). Cytoplasmic staining on rat spleen is observed. The nuclear counterstain was DAPI (Blue).

Secondary antibody control : Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 (2 ug/ml) dilution.

Immunohistochemistry (Frozen sections) - Anti-CCR7 antibody [EPR23192-57] (AB253187)
  • IHC-Fr

Unknown

Immunohistochemistry (Frozen sections) - Anti-CCR7 antibody [EPR23192-57] (AB253187)

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse spleen tissue labeling CCR7 with ab253187 at 1/500 dilution followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 (2 ug/ml) dilution (Green). Cytoplasmic staining on mouse spleen is observed. The nuclear counterstain was DAPI (Blue).

Secondary antibody control : Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 (2 ug/ml) dilution.

Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).<\p>

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CCR7 antibody [EPR23192-57] (AB253187)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CCR7 antibody [EPR23192-57] (AB253187)

Immunohistochemical analysis of paraffin-embedded Rat spleen tissue labeling CCR7 with ab253187 at 1/500 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining in rat spleen. The section was incubated with ab253187 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND RX instrument Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins

Immunohistochemistry (Frozen sections) - Anti-CCR7 antibody [EPR23192-57] (AB253187)
  • IHC-Fr

Unknown

Immunohistochemistry (Frozen sections) - Anti-CCR7 antibody [EPR23192-57] (AB253187)

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse pancreas tissue labeling CCR7 with ab253187 at 1/100 dilution followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 (2 ug/ml) dilution (Green). Negative control : No staining on mouse pancreas is observed. The nuclear counterstain was DAPI (Blue).

Secondary antibody control : Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 (2 ug/ml) dilution.

  • 421 Alexa Fluor® 405

    Donkey Anti-Rabbit IgG H&L (Alexa Fluor® 405) preadsorbed

  • 421 Alexa Fluor® 405

    Goat Anti-Rabbit IgG H&L (Alexa Fluor® 405) preadsorbed

  • 565 Alexa Fluor® 555

    Goat Anti-Rabbit IgG H&L (Alexa Fluor® 555) preadsorbed

  • 603 Alexa Fluor® 568

    Goat Anti-Rabbit IgG H&L (Alexa Fluor® 568) preadsorbed

  • 617 Alexa Fluor® 594

    Donkey Anti-Rabbit IgG H&L (Alexa Fluor® 594) preadsorbed

  • 617 Alexa Fluor® 594

    Goat Anti-Rabbit IgG H&L (Alexa Fluor® 594) preadsorbed

  • Biotin

    Goat Anti-Rabbit IgG H&L (Biotin)

  • Biotin

    Donkey Anti-Rabbit IgG H&L (Biotin)

  • HRP

    Goat Anti-Rabbit IgG H&L (HRP)

  • HRP

    Donkey Anti-Rabbit IgG H&L (HRP)

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR23192-57

Isotype

IgG

Carrier free

No

Reacts with

Mouse, Rat, Human

Applications

IHC-P, IHC-Fr

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD4 antibody [EPR6855] (AB133616)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CD11c antibody [EP1347Y] - C-terminal (AB52632)

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Anti-CD14 antibody [SP192]

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  • 5
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Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/500", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" }, "Mouse": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/500", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCFr-species-checked": "testedAndGuaranteed", "IHCFr-species-dilution-info": "1/100", "IHCFr-species-notes": "<p>Perform heat mediated antigen retrieval using sodium citrate buffer (10 mM citrate pH 6.0 and 0.05% Tween-20).</p>" }, "Rat": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "1/500", "IHCP-species-notes": "<p></p> Perform heat-mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCFr-species-checked": "testedAndGuaranteed", "IHCFr-species-dilution-info": "1/100", "IHCFr-species-notes": "<p>Perform heat mediated antigen retrieval using sodium citrate buffer (10 mM citrate pH 6.0 and 0.05% Tween-20).</p>" } } }
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Product details

What is this antibody validated in?
Anti-CCR7 antibody [EPR23192-57] (ab253187) is a rabbit recombinant monoclonal antibody and is validated for use in Immunohistochemistry (IHC-P), Immunohistochemistry (IHC-Fr) in Human, Mouse, Rat samples.

Trial sizes available!
Test your antibody or perform pre-screening before committing to a larger quantity. Sold in 10µl. Discover our selection of trial-size antibodies.

Other related products
We have a range of other formats of antibody clone [EPR23192-57] also available for your convenience: ab253187, Carrier free - ab272938, Alexa Fluor® 647 - ab275165, ab284437, Alexa Fluor® 488 - ab311015, Alexa Fluor® 594 - ab311781, Alexa Fluor® 568 - ab313062, Alexa Fluor® 555 - ab313262, Alexa Fluor® 750 - ab321499

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Preservative: 0.01% Sodium azide Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CCR7 also known as CD197 is a chemokine receptor with a molecular mass of approximately 43-48 kDa. The receptor is known to be expressed predominantly in lymphoid tissues such as the spleen and lymph nodes. Additionally CCR7 is found on various immune cells like T cells and dendritic cells. Researchers study CCR7 using different methods such as CCR7-PE and CCR7-APC antibody staining in flow cytometry to examine its expression on immune cells in various conditions.
Biological function summary

CCR7 plays an important role in directing the migration and homeostasis of T cells and dendritic cells. It is not part of a larger protein complex but interacts functionally with specific signaling enzymes upon activation. CCR7 is important for the function of the immune system aiding in the development of adaptive immunity by controlling lymphocyte movement and positioning in lymphoid tissues. This receptor assists in guiding cells to specific lymphoid organs where immune responses are initiated and maintained.

Pathways

CCR7 is significantly involved in the CCL19/CCL21 chemokine signaling pathways which help direct immune cell positioning. CCR7 interacts with these chemokines to regulate the trafficking of cells to lymphoid tissues. Additionally it works in tandem with other receptors like CCR5 in the regulation of immune cell mobilization. These pathways are essential in coordinating the immune response during infection and inflammation.

CCR7 is connected with cancer metastasis and chronic inflammatory diseases. Its role in cell migration makes it relevant in the metastasis of certain cancers where cells misregulate their movement and spread from the original tumor site. Additionally CCR7's involvement in chronic inflammatory diseases highlights its connection to rheumatoid arthritis where it may interact with other proteins such as CCL19 and CCL21 contributing to disease progression. Understanding these interactions helps develop potential therapeutic strategies targeting CCR7 in such diseases.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Receptor for the MIP-3-beta chemokine. Probable mediator of EBV effects on B-lymphocytes or of normal lymphocyte functions.
See full target information CCR7
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Publications (18)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 16:6578 PubMed40675962

2025

The tumor-sentinel lymph node immuno-migratome reveals CCR7⁺ dendritic cells drive response to sequenced immunoradiotherapy.

Applications

Unspecified application

Species

Unspecified reactive species

Robert Saddawi-Konefka,Riyam Al Msari,Shiqi Tang,Chad Philips,Sayed Sadat,Lauren M Clubb,Sarah Luna,Santiago Fassardi,Riley Jones,Ida Franiak Pietryga,Farhoud Faraji,Shiruyeh Schokrpur,Bryan S Yung,Michael M Allevato,Kelsey E Decker,Chanond A Nasamran,Daisy Chilin-Fuentes,Sara Brin Rosenthal,Shawn M Jensen,Bernard A Fox,R Bryan Bell,J Silvio Gutkind,Andrew Sharabi,Joseph A Califano

Bioactive materials 50:232-245 PubMed40276540

2025

A multifunctional trap-capture-kill antibacterial system for enhanced wound healing via modified decellularized mushroom aerogels.

Applications

Unspecified application

Species

Unspecified reactive species

Chuwei Zhang,Shuai Fan,Jing Zhang,Ganghua Yang,Chao Cai,Shixuan Chen,Yongjin Fang,Wenbing Wan

Oncology research 33:199-212 PubMed39735670

2024

Lenalidomide regulates the CCL21/CCR7/ERK1/2 axis to inhibit migration and proliferation in diffuse large B-cell lymphoma.

Applications

Unspecified application

Species

Unspecified reactive species

Wen Yang,Bin Tang,Dan Xu,Wenxiu Yang

Advanced science (Weinheim, Baden-Wurttemberg, Germany) 12:e2411575 PubMed39686701

2024

Thermosensitive Hydrogel Integrated with Bimetallic Nano-Enzymes for Modulating the Microenvironment in Diabetic Wound Beds.

Applications

Unspecified application

Species

Unspecified reactive species

Chuwei Zhang,Xiaoyi Zhang,Fei Li,Bo Li,Mengnan Zhang,Wanqian Li,Pan Zhuge,Jingye Yao,Yi Zhang,Shixuan Chen,Yongjin Fang,Chao Cai

Journal of nanobiotechnology 22:691 PubMed39523339

2024

Silver nanoparticle induced immunogenic cell death can improve immunotherapy.

Applications

Unspecified application

Species

Unspecified reactive species

Ara Sargsian,Xanthippi Koutsoumpou,Hermon Girmatsion,Can Egil,Kiana Buttiens,Carla Rios Luci,Stefaan J Soenen,Bella B Manshian

Cell discovery 10:103 PubMed39414763

2024

Single-cell spatiotemporal analysis reveals alveolar dendritic cell-T cell immunity hubs defending against pulmonary infection.

Applications

Unspecified application

Species

Unspecified reactive species

Boyi Cong,Xuan Dong,Zongheng Yang,Pin Yu,Yangyang Chai,Jiaqi Liu,Meihan Zhang,Yupeng Zang,Jingmin Kang,Yu Feng,Yi Liu,Weimin Feng,Dehe Wang,Wei Deng,Fengdi Li,Zhiqi Song,Ziqiao Wang,Xiaosu Chen,Hua Qin,Qinyi Yu,Zhiqing Li,Shuxun Liu,Xun Xu,Nanshan Zhong,Xianwen Ren,Chuan Qin,Longqi Liu,Jian Wang,Xuetao Cao

Materials today. Bio 28:101237 PubMed39315393

2024

A 3D radially aligned nanofiber scaffold co-loaded with LL37 mimetic peptide and PDGF-BB for the management of infected chronic wounds.

Applications

Unspecified application

Species

Unspecified reactive species

Fei Li,Chuwei Zhang,Xiaoping Zhong,Bo Li,Mengnan Zhang,Wanqian Li,Lifei Zheng,Xinghua Zhu,Shixuan Chen,Yi Zhang

Bioactive materials 39:582-594 PubMed38883316

2024

Orthogonally woven 3D nanofiber scaffolds promote rapid soft tissue regeneration by enhancing bidirectional cell migration.

Applications

Unspecified application

Species

Unspecified reactive species

Jiayi Yuan,Bingbing Sun,Weixing Ma,Chao Cai,Zhenzhen Huang,Peiyi Zhou,Lei Yi,Lubin Liu,Shixuan Chen

Cancer immunology, immunotherapy : CII 73:131 PubMed38748299

2024

GGT5: a potential immunotherapy response inhibitor in gastric cancer by modulating GSH metabolism and sustaining memory CD8+ T cell infiltration.

Applications

Unspecified application

Species

Unspecified reactive species

Wenjing Zhao,Ziwei Liang,Yongshi Yao,Yang Ge,Guangyu An,Ling Duan,Jiannan Yao

Advanced science (Weinheim, Baden-Wurttemberg, Germany) 11:e2308077 PubMed38403462

2024

Poly(Glutamic Acid-Lysine) Hydrogels with Alternating Sequence Resist the Foreign Body Response in Rodents and Non-Human Primates.

Applications

Unspecified application

Species

Unspecified reactive species

Xianchi Zhou,Wenzhong Cao,Yongcheng Chen,Zihao Zhu,Yifeng Chen,Yanwen Ni,Zuolong Liu,Fan Jia,Zhouyu Lu,Yang Ye,Haijie Han,Ke Yao,Weifeng Liu,Xinyue Wei,Shengfu Chen,Youxiang Wang,Jian Ji,Peng Zhang
View all publications
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Product promise

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