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AB272938

Anti-CCR7 antibody [EPR23192-57] - BSA and Azide free

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Anti-CCR7 antibody [EPR23192-57] - BSA and Azide free (ab272938) is a rabbit recombinant monoclonal antibody provided in a PBS only buffer for easy conjugation detecting CCR7 in IHC-P, IHC-Fr. Suitable for Human, Mouse, Rat.

- BSA, sodium azide, and glycerol-free for easy conjugation
- Biophysical QC for unrivalled batch-batch consistency

View Alternative Names

CD197, CMKBR7, EBI1, EVI1, CCR7, C-C chemokine receptor type 7, C-C CKR-7, CC-CKR-7, CCR-7, BLR2, CDw197, Epstein-Barr virus-induced G-protein coupled receptor 1, MIP-3 beta receptor, EBV-induced G-protein coupled receptor 1

9 Images
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CCR7 antibody [EPR23192-57] - BSA and Azide free (AB272938)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CCR7 antibody [EPR23192-57] - BSA and Azide free (AB272938)

Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling CCR7 with ab253187 at 1/500 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining in human tonsil. The section was incubated with ab253187 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND RX instrument Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab253187).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CCR7 antibody [EPR23192-57] - BSA and Azide free (AB272938)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CCR7 antibody [EPR23192-57] - BSA and Azide free (AB272938)

Immunohistochemical analysis of paraffin-embedded Human pancreas tissue labeling CCR7 with ab253187 at 1/500 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Negative control : No staining in human pancreas. The section was incubated with ab253187 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND RX instrument Counterstained with Hematoxylin.

Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab253187).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CCR7 antibody [EPR23192-57] - BSA and Azide free (AB272938)
  • IHC-P

Lab

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CCR7 antibody [EPR23192-57] - BSA and Azide free (AB272938)

Immunohistochemical analysis of formalin fixed paraffin embedded human tonsil labelling CCR7 with ab272938 at a concentration of 1µg/ml. The immunostaining was performed on a Ventana DISCOVERY ULTRA (Roche Tissue Diagnostics) instrument with an OptiView DAB IHC Detection Kit. Heat mediated antigen retrieval was conducted for 32 min with ULTRA cell conditioning solution (CC1 pH 8.5). ab272938 anti CCR7 antibody was incubated at 37°C for 16min. Sections were counterstained is with Hematoxylin II. Image inset shows absence of staining in secondary antibody only control.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CCR7 antibody [EPR23192-57] - BSA and Azide free (AB272938)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CCR7 antibody [EPR23192-57] - BSA and Azide free (AB272938)

Immunohistochemical analysis of paraffin-embedded Human Hodgkin's lymphoma tissue labeling CCR7 with ab253187 at 1/500 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining on RS cells in human Hodgkin's lymphoma. The section was incubated with ab253187 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND RX instrument Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab253187).

Immunohistochemistry (Frozen sections) - Anti-CCR7 antibody [EPR23192-57] - BSA and Azide free (AB272938)
  • IHC-Fr

Unknown

Immunohistochemistry (Frozen sections) - Anti-CCR7 antibody [EPR23192-57] - BSA and Azide free (AB272938)

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse pancreas tissue labeling CCR7 with ab253187 at 1/100 dilution followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 (2 ug/ml) dilution (Green). Negative control : No staining on mouse pancreas is observed. The nuclear counterstain was DAPI (Blue).

Secondary antibody control : Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 (2 ug/ml) dilution.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab253187).

Immunohistochemistry (Frozen sections) - Anti-CCR7 antibody [EPR23192-57] - BSA and Azide free (AB272938)
  • IHC-Fr

Unknown

Immunohistochemistry (Frozen sections) - Anti-CCR7 antibody [EPR23192-57] - BSA and Azide free (AB272938)

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse spleen tissue labeling CCR7 with ab253187 at 1/500 dilution followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 (2 ug/ml) dilution (Green). Cytoplasmic staining on mouse spleen is observed. The nuclear counterstain was DAPI (Blue).

Secondary antibody control : Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 (2 ug/ml) dilution.

Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).<\p>

Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).<\p>

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab253187).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CCR7 antibody [EPR23192-57] - BSA and Azide free (AB272938)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CCR7 antibody [EPR23192-57] - BSA and Azide free (AB272938)

Immunohistochemical analysis of paraffin-embedded Mouse spleen tissue labeling CCR7 with ab253187 at 1/500 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining in mouse spleen. The section was incubated with ab253187 for 30 mins at room temperature.The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab253187).

Immunohistochemistry (Frozen sections) - Anti-CCR7 antibody [EPR23192-57] - BSA and Azide free (AB272938)
  • IHC-Fr

Unknown

Immunohistochemistry (Frozen sections) - Anti-CCR7 antibody [EPR23192-57] - BSA and Azide free (AB272938)

Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Rat spleen tissue labeling CCR7 with ab253187 at 1/500 dilution followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 (2 ug/ml) dilution (Green). Cytoplasmic staining on rat spleen is observed. The nuclear counterstain was DAPI (Blue).

Secondary antibody control : Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 (2 ug/ml) dilution.

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab253187).

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CCR7 antibody [EPR23192-57] - BSA and Azide free (AB272938)
  • IHC-P

Unknown

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CCR7 antibody [EPR23192-57] - BSA and Azide free (AB272938)

Immunohistochemical analysis of paraffin-embedded Rat spleen tissue labeling CCR7 with ab253187 at 1/500 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Positive staining in rat spleen. The section was incubated with ab253187 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND RX instrument Counterstained with Hematoxylin.

Secondary antibody only control : Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

Heat mediated antigen retrieval with Citrate buffer (pH 6.0, epitope retrieval solution 1) for 20 mins

This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab253187).

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR23192-57

Isotype

IgG

Carrier free

Yes

Reacts with

Mouse, Rat, Human

Applications

IHC-Fr, IHC-P

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "IHCP" : {"fullname" : "Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)", "shortname":"IHC-P"}, "IP" : {"fullname" : "Immunoprecipitation", "shortname":"IP"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "WB" : {"fullname" : "Western blot", "shortname":"WB"}, "IHCFr" : {"fullname" : "Immunohistochemistry (Frozen sections)", "shortname":"IHC-Fr"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCFr-species-checked": "guaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "" }, "Mouse": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCFr-species-checked": "testedAndGuaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "<p></p>" }, "Rat": { "IHCP-species-checked": "testedAndGuaranteed", "IHCP-species-dilution-info": "", "IHCP-species-notes": "<p></p>", "IP-species-checked": "notRecommended", "IP-species-dilution-info": "", "IP-species-notes": "<p></p>", "FlowCyt-species-checked": "notRecommended", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>", "WB-species-checked": "notRecommended", "WB-species-dilution-info": "", "WB-species-notes": "<p></p>", "IHCFr-species-checked": "testedAndGuaranteed", "IHCFr-species-dilution-info": "", "IHCFr-species-notes": "<p></p>" } } }
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Product details

What is this antibody validated in?
Anti-CCR7 antibody [EPR23192-57] - BSA and Azide free (ab272938) is a rabbit recombinant monoclonal antibody and is validated for use in Immunohistochemistry (IHC-P), Immunohistochemistry (IHC-Fr) in Human, Mouse, Rat samples.

Other related products
We have a range of other formats of antibody clone [EPR23192-57] also available for your convenience: ab253187, Carrier free - ab272938, Alexa Fluor® 647 - ab275165, ab284437, Alexa Fluor® 488 - ab311015, Alexa Fluor® 594 - ab311781, Alexa Fluor® 568 - ab313062, Alexa Fluor® 555 - ab313262, Alexa Fluor® 750 - ab321499

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
Storage information
Do Not Freeze
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CCR7 also known as CD197 is a chemokine receptor with a molecular mass of approximately 43-48 kDa. The receptor is known to be expressed predominantly in lymphoid tissues such as the spleen and lymph nodes. Additionally CCR7 is found on various immune cells like T cells and dendritic cells. Researchers study CCR7 using different methods such as CCR7-PE and CCR7-APC antibody staining in flow cytometry to examine its expression on immune cells in various conditions.
Biological function summary

CCR7 plays an important role in directing the migration and homeostasis of T cells and dendritic cells. It is not part of a larger protein complex but interacts functionally with specific signaling enzymes upon activation. CCR7 is important for the function of the immune system aiding in the development of adaptive immunity by controlling lymphocyte movement and positioning in lymphoid tissues. This receptor assists in guiding cells to specific lymphoid organs where immune responses are initiated and maintained.

Pathways

CCR7 is significantly involved in the CCL19/CCL21 chemokine signaling pathways which help direct immune cell positioning. CCR7 interacts with these chemokines to regulate the trafficking of cells to lymphoid tissues. Additionally it works in tandem with other receptors like CCR5 in the regulation of immune cell mobilization. These pathways are essential in coordinating the immune response during infection and inflammation.

CCR7 is connected with cancer metastasis and chronic inflammatory diseases. Its role in cell migration makes it relevant in the metastasis of certain cancers where cells misregulate their movement and spread from the original tumor site. Additionally CCR7's involvement in chronic inflammatory diseases highlights its connection to rheumatoid arthritis where it may interact with other proteins such as CCL19 and CCL21 contributing to disease progression. Understanding these interactions helps develop potential therapeutic strategies targeting CCR7 in such diseases.
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Receptor for the MIP-3-beta chemokine. Probable mediator of EBV effects on B-lymphocytes or of normal lymphocyte functions.
See full target information CCR7
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com