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AB324532

Anti-CCR7 antibody [EPR29703-53] - BSA and Azide free

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Rabbit Recombinant Monoclonal CCR7 antibody. Carrier free. Suitable for Flow Cyt and reacts with Human samples.

View Alternative Names

CD197, CMKBR7, EBI1, EVI1, CCR7, C-C chemokine receptor type 7, C-C CKR-7, CC-CKR-7, CCR-7, BLR2, CDw197, Epstein-Barr virus-induced G-protein coupled receptor 1, MIP-3 beta receptor, EBV-induced G-protein coupled receptor 1

4 Images
Flow Cytometry - Anti-CCR7 antibody [EPR29703-53] - BSA and Azide free (AB324532)
  • Flow Cyt

Supplier Data

Flow Cytometry - Anti-CCR7 antibody [EPR29703-53] - BSA and Azide free (AB324532)

This data was developed using ab324521, the same antibody clone in a different buffer formulation.

Flow cytometric analysis of Human PBMC (human peripheral blood mononuclear cell) cells labelling CCR7 with ab324521 at 1/500 dilution (0.1ug) / Right compared with a Rabbit monoclonal IgG (ab172730) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).

Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/5000 dilution was used as the secondary antibody.

Gated on viable cells.
Cells are co-stained with CD19 conjugated to Alexa Fluor® 647.

Flow Cytometry - Anti-CCR7 antibody [EPR29703-53] - BSA and Azide free (AB324532)
  • Flow Cyt

Supplier Data

Flow Cytometry - Anti-CCR7 antibody [EPR29703-53] - BSA and Azide free (AB324532)

This data was developed using ab324521, the same antibody clone in a different buffer formulation.

Flow cytometric analysis of Human PBMC (human peripheral blood mononuclear cell) cells labelling CCR7 with ab324521 at 1/500 dilution (0.1ug) / Right compared with a Rabbit monoclonal IgG (ab172730) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).

Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/5000 dilution was used as the secondary antibody.

Gated on viable cells.
Cells are co-stained with CD3 conjugated to Alexa Fluor® 647.

Flow Cytometry - Anti-CCR7 antibody [EPR29703-53] - BSA and Azide free (AB324532)
  • Flow Cyt

Supplier Data

Flow Cytometry - Anti-CCR7 antibody [EPR29703-53] - BSA and Azide free (AB324532)

This data was developed using ab324521, the same antibody clone in a different buffer formulation.

Flow cytometric analysis of Human PBMC (human peripheral blood mononuclear cell) cells labelling CCR7 with ab324521 at 1/500 dilution (0.1ug) / Right compared with a Rabbit monoclonal IgG (ab172730) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).

Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/5000 dilution was used as the secondary antibody.

Gated on viable cells.
Cells are co-stained with CD56 conjugated to PE.

Flow Cytometry - Anti-CCR7 antibody [EPR29703-53] - BSA and Azide free (AB324532)
  • Flow Cyt

Supplier Data

Flow Cytometry - Anti-CCR7 antibody [EPR29703-53] - BSA and Azide free (AB324532)

This data was developed using ab324521, the same antibody clone in a different buffer formulation.

Flow cytometric analysis of Human PBMC (human peripheral blood mononuclear cell) cells labelling CCR7 with ab324521 at 1/500 dilution (0.1ug) / Right compared with a Rabbit monoclonal IgG (ab172730) / Left isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody).

Goat Anti-Rabbit IgG (Alexa Fluor® 488, ab150081) at 1/5000 dilution was used as the secondary antibody.

Gated on viable cells.
Cells are co-stained with CD14 conjugated to PE.

Key facts

Host species

Rabbit

Clonality

Monoclonal

Clone number

EPR29703-53

Isotype

IgG

Carrier free

Yes

Reacts with

Human

Applications

Flow Cyt

applications

Immunogen

The exact immunogen used to generate this antibody is proprietary information.

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "<p></p>" } } }
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Product details

ab324532 is the carrier-free version of ab324521.

Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.

Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.

Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:

  • - High batch-to-batch consistency and reproducibility
  • - Improved sensitivity and specificity
  • - Long-term security of supply
  • - Animal-free batch production

For more information, read more on recombinant antibodies.

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Properties and storage information

Form
Liquid
Purification technique
Affinity purification Protein A
Storage buffer
pH: 7.2 - 7.4 Constituents: PBS
Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
+4°C
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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
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Target data

Receptor for the MIP-3-beta chemokine. Probable mediator of EBV effects on B-lymphocytes or of normal lymphocyte functions.
See full target information CCR7
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com