Rabbit Recombinant Monoclonal CCR8 antibody. Carrier free. Suitable for IP, ELISA, WB and reacts with Human, Synthetic peptide - Human samples.
Images
![Immunoprecipitation - Anti-CCR8 antibody [E77] - BSA and Azide free (AB239814), expandable thumbnail](https://content.abcam.com/products/images/ccr8-antibody-e77-bsa-and-azide-free-ab239814--immunoprecipitation-img11723.jpg/_jcr_content/renditions/webp-475.webp "Immunoprecipitation - Anti-CCR8 antibody [E77] - BSA and Azide free (AB239814)")
![Western blot - Anti-CCR8 antibody [E77] - BSA and Azide free (AB239814), expandable thumbnail](https://content.abcam.com/products/images/ccr8-antibody-e77-bsa-and-azide-free-ab239814--western-blot-img4218.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-CCR8 antibody [E77] - BSA and Azide free (AB239814)")
![ELISA - Anti-CCR8 antibody [E77] - BSA and Azide free (AB239814), expandable thumbnail](https://content.abcam.com/products/images/ccr8-antibody-e77-bsa-and-azide-free-ab239814--elisa-img77113.jpg/_jcr_content/renditions/webp-475.webp "ELISA - Anti-CCR8 antibody [E77] - BSA and Azide free (AB239814)")
![Western blot - Anti-CCR8 antibody [E77] - BSA and Azide free (AB239814), expandable thumbnail](https://content.abcam.com/products/images/ccr8-antibody-e77-bsa-and-azide-free-ab239814--western-blot-img159269.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-CCR8 antibody [E77] - BSA and Azide free (AB239814)")
![Western blot - Anti-CCR8 antibody [E77] - BSA and Azide free (AB239814), expandable thumbnail](https://content.abcam.com/products/images/ccr8-antibody-e77-bsa-and-azide-free-ab239814--western-blot-img28623.jpg/_jcr_content/renditions/webp-475.webp "Western blot - Anti-CCR8 antibody [E77] - BSA and Azide free (AB239814)")
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
Reactivity data
Select an application| IP | ELISA | WB | Flow Cyt | ICC/IF | |
|---|---|---|---|---|---|
| Human | Tested | Expected | Tested | Not recommended | Not recommended |
| Synthetic peptide - Human | Not recommended | Tested | Not recommended | Not recommended | Not recommended |
IP
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Synthetic peptide - Human | Dilution info - | Notes - |
ELISA
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Synthetic peptide - Human | Dilution info - | Notes - |
ExpectedExpected
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info Use at an assay dependent concentration. | Notes - |
WB
TestedTested
| Species | Dilution info | Notes |
|---|---|---|
Species Human | Dilution info - | Notes - |
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Synthetic peptide - Human | Dilution info - | Notes - |
Flow Cyt
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Synthetic peptide - Human | Dilution info - | Notes - |
ICC/IF
Not recommendedNot recommended
| Species | Dilution info | Notes |
|---|---|---|
Species Human, Synthetic peptide - Human | Dilution info - | Notes - |
Associated Products
Select an associated product type
3 products for Alternative Product
Target data
Function
Receptor for the chemokine CCL1/SCYA1/I-309. May regulate monocyte chemotaxis and thymic cell line apoptosis. Alternative coreceptor with CD4 for HIV-1 infection.
Alternative names
CDw198, CKRL1, CMKBR8, CMKBRL2, CCR8, C-C chemokine receptor type 8, C-C CKR-8, CC-CKR-8, CCR-8, CC chemokine receptor CHEMR1, Chemokine receptor-like 1, GPR-CY6, TER1, CKR-L1, GPRCY6
Recommended products
Rabbit Recombinant Monoclonal CCR8 antibody. Carrier free. Suitable for IP, ELISA, WB and reacts with Human, Synthetic peptide - Human samples.
Key facts
- Isotype
- IgG
- Host species
- Rabbit
- Storage buffer
pH: 7.2 - 7.4
Constituents: PBS- Form
- Liquid
- Clonality
- Monoclonal
- Immunogen
- The exact immunogen used to generate this antibody is proprietary information.
- Carrier free
- Yes
- Clone number
- E77
- Purification technique
- Affinity purification Protein A
- Specificity
Does not cross-react with other G-protein coupled receptor 1 family members.
- Epitope
- Residues in loop 2 extracellular domain of human CCR8
- Concentration
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- +4°C
- Storage information
- Do Not Freeze
Notes
ab239814 is the carrier-free version of Anti-CCR8 antibody [E77] ab32399.
Species reactivity
Mouse: We have preliminary internal testing data to indicate this antibody may not react with this species.
Please contact us for more information.
Patented technology
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
What are the advantages of a recombinant monoclonal antibody?
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free batch production
For more information, read more on recombinant antibodies.
Conjugation ready
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with 1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Compatibility
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The CC chemokine receptor 8 abbreviated as CCR8 is a G protein-coupled receptor with an approximate molecular mass of 41 kDa. It is also known as CDw198. Expression of CCR8 protein is observed mainly in T cells particularly CD4+ T cells and natural killer cells. Antibody against CCR8 like anti-CCR8 can be used to study the expression of this receptor in tissues via immunohistochemistry (IHC) which may be referred to as CCR8 IHC.
- Biological function summary
CCR8 plays a role in immune cell signaling and migration. This receptor interacts with its ligand CCL1 to mediate chemotaxis guiding cells to sites of inflammation or injury. CCR8 forms a part of the immune response machinery facilitating communication between cells. Through these interactions it influences the function of various immune cells such as regulatory T cells and supports the maintenance of immune homeostasis.
- Pathways
CCR8 is involved in chemokine signaling pathways that regulate immune responses. It operates within the broader context of the chemokine receptor family including receptors like CCR4. These pathways are important for directing lymphocyte migration and are implicated in inflammation and immune surveillance processes. The engagement of CCR8 modulates downstream signaling events that influence T cell differentiation and immune cell recruitment.
- Associated diseases and disorders
Alterations in CCR8 expression or function relate to inflammatory diseases and certain cancers. Aberrant expression of CCR8 associates with disorders such as autoimmune diseases and Hodgkin's lymphoma. In cancer the cross-talk between CCR8 and its ligands can promote a protumorigenic environment aiding tumor progression. Connections exist between CCR8 and other receptors like CCR4 in these pathologies highlighting its role in disease development and potential as a therapeutic target.
Product promise
Tested
We have tested this species and application combination and it works. It is covered by our product promise.
Expected
We have not tested this specific species and application combination in-house, but expect it will work. It is covered by our product promise.
Predicted
This species and application combination has not been tested, but we predict it will work based on strong homology. However, this combination is not covered by our product promise.
Not recommended
We do not recommend this combination. It is not covered by our product promise.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
5 product images
![Immunoprecipitation - Anti-CCR8 antibody [E77] - BSA and Azide free (ab239814), expandable thumbnail](https://content.abcam.com/products/images/ccr8-antibody-e77-bsa-and-azide-free-ab239814--immunoprecipitation-img11723.jpg "Immunoprecipitation - Anti-CCR8 antibody [E77] - BSA and Azide free (ab239814)")
Immunoprecipitation - Anti-CCR8 antibody [E77] - BSA and Azide free (ab239814)
This data was developed using Anti-CCR8 antibody [E77] ab32399, the same antibody clone in a different buffer formulation.Anti-CCR8 antibody [E77] ab32399 (purified) at 1/110 dilution (20 µg/mL) immunoprecipitating CCR8 in Human thymus lysate. Human thymus lysate 10 µgab32399 & Human thymus lysateRabbit monoclonal IgG (Rabbit IgG, monoclonal [EPR25A] - Isotype Control ab172730) instead of Anti-CCR8 antibody [E77] ab32399 in Human thymus lysate
For western blotting, Anti-CCR8 antibody [E77] ab32399 at 1/500 dilution (4.45 µg/mL) and VeriBlot for IP Detection Reagent (HRP) (VeriBlot for IP Detection Reagent (HRP) ab131366) was used for detection at 1/1000 dilution.
Blocking and diluting buffer: 5% NFDM /TBST .All lanes: Immunoprecipitation - Anti-CCR8 antibody [E77] (Anti-CCR8 antibody [E77] ab32399)
Predicted band size: 41 kDa
![Western blot - Anti-CCR8 antibody [E77] - BSA and Azide free (ab239814), expandable thumbnail](https://content.abcam.com/products/images/ccr8-antibody-e77-bsa-and-azide-free-ab239814--western-blot-img4218.jpg "Western blot - Anti-CCR8 antibody [E77] - BSA and Azide free (ab239814)")
Western blot - Anti-CCR8 antibody [E77] - BSA and Azide free (ab239814)
This data was developed using Anti-CCR8 antibody [E77] ab32399, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
All lanes: Western blot - Anti-CCR8 antibody [E77] (Anti-CCR8 antibody [E77] ab32399) at 1/1000 dilution
All lanes: A431 (Human epidermoid carcinoma epithelial cell) whole cell lysates at 15 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/20000 dilution
Predicted band size: 41 kDa
Observed band size: 41 kDa
![ELISA - Anti-CCR8 antibody [E77] - BSA and Azide free (ab239814), expandable thumbnail](https://content.abcam.com/products/images/ccr8-antibody-e77-bsa-and-azide-free-ab239814--elisa-img77113.jpg "ELISA - Anti-CCR8 antibody [E77] - BSA and Azide free (ab239814)")
ELISA - Anti-CCR8 antibody [E77] - BSA and Azide free (ab239814)
This data was developed using Anti-CCR8 antibody [E77] ab32399, the same antibody clone in a different buffer formulation.ELISA using Human CCR8 Antigen at a concentration of 1000 ng/ml; and purified Anti-CCR8 antibody [E77] ab32399 at 1000-0 ng/ml. Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG (H+L) was used as the secondary antibody at a 1:2500 dilution.
![Western blot - Anti-CCR8 antibody [E77] - BSA and Azide free (ab239814), expandable thumbnail](https://content.abcam.com/products/images/ccr8-antibody-e77-bsa-and-azide-free-ab239814--western-blot-img159269.jpg "Western blot - Anti-CCR8 antibody [E77] - BSA and Azide free (ab239814)")
Western blot - Anti-CCR8 antibody [E77] - BSA and Azide free (ab239814)
This data was developed using Anti-CCR8 antibody [E77] ab32399, the same antibody clone in a different buffer formulation.
All lanes: Western blot - Anti-CCR8 antibody [E77] (Anti-CCR8 antibody [E77] ab32399) at 1/5000 dilution
All lanes: Human Thymus lysate at 15 µg
SecondaryAll lanes: Goat Anti-Rabbit IgG, (H+L), Peroxidase at 1/20000 dilution
Predicted band size: 41 kDa
Observed band size: 41 kDa
![Western blot - Anti-CCR8 antibody [E77] - BSA and Azide free (ab239814), expandable thumbnail](https://content.abcam.com/products/images/ccr8-antibody-e77-bsa-and-azide-free-ab239814--western-blot-img28623.jpg "Western blot - Anti-CCR8 antibody [E77] - BSA and Azide free (ab239814)")
Western blot - Anti-CCR8 antibody [E77] - BSA and Azide free (ab239814)
This data was developed using Anti-CCR8 antibody [E77] ab32399, the same antibody clone in a different buffer formulation.
All lanes: Western blot - Anti-CCR8 antibody [E77] (Anti-CCR8 antibody [E77] ab32399) at 1/5000 dilution
All lanes: Human spleen tissue lysate at 15 µg
SecondaryAll lanes: Goat anti-Rabbit IgG, (H+L), peroxidase conjugated at 1/20000 dilution
Predicted band size: 41 kDa
Observed band size: 41 kDa
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