AB52590

Anti-CCR9 antibody [7E7]

Name: Anti-CCR9 antibody [7E7] (ab52590) | Abcam
Brand: Abcam Limited
SKU: AB52590
Price: 525 USD
Availability: InStock

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(1 Publication)

Rat Monoclonal CCR9 antibody. Suitable for Flow Cyt, ICC/IF and reacts with Human, Mouse samples. Cited in 1 publication. Immunogen corresponding to Synthetic Peptide within Mouse Ccr9 aa 1-50.

View Alternative Names

CDw199, Cmkbr10, Ccr9, C-C chemokine receptor type 9, C-C CKR-9, CC-CKR-9, CCR-9, Chemokine C-C receptor 10

2 Images

Flow Cytometry - Anti-CCR9 antibody [7E7] (AB52590)

Flow Cyt

Unknown

Flow Cytometry - Anti-CCR9 antibody [7E7] (AB52590)

Overlay histogram showing K562 cells stained with ab52590 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab52590, 1/50 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-rat IgG (H+L) (ab98386) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rat IgG2b [RTK4530] (2μg/1x10⁶ cells) used under the same conditions. Acquisition of >5,000 events was performed.

Immunocytochemistry/ Immunofluorescence - Anti-CCR9 antibody [7E7] (AB52590)

ICC/IF

Supplier Data

Immunocytochemistry/ Immunofluorescence - Anti-CCR9 antibody [7E7] (AB52590)

ab52590 staining CCR9 in Mouse brain tissue sections by Immunocytochemistry/ Immunofluorescence.

Red = CCR9

Green = IgA

Blue = CD3

Key facts

Host species

Rat

Clonality

Monoclonal

Clone number

7E7

Isotype

IgG2b

Carrier free

Reacts with

Mouse, Human

Applications

ICC/IF, Flow Cyt

applications

Immunogen

Synthetic Peptide within Mouse Ccr9 aa 1-50. The exact immunogen used to generate this antibody is proprietary information.

Q9WUT7

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Species", "Dilution Info", "Notes"], "tabs": { "all-applications": {"fullname" : "All Applications", "shortname": "All Applications"}, "FlowCyt" : {"fullname" : "Flow Cytometry", "shortname":"Flow Cyt"}, "ICCIF" : {"fullname" : "Immunocytochemistry/ Immunofluorescence", "shortname":"ICC/IF"} }, "product-promise": { "all": "all", "testedAndGuaranteed": "tested", "guaranteed": "expected", "predicted": "predicted", "notRecommended": "not-recommended" } }, "values": { "Human": { "FlowCyt-species-checked": "testedAndGuaranteed", "FlowCyt-species-dilution-info": "1/20", "FlowCyt-species-notes": "<p><a href='/en-us/products/unavailable/rat-igg2b-kappa-monoclonal-rtk4530-isotype-control-bsa-and-azide-free-ab18536'>ab18536</a> - Rat monoclonal IgG2b, is suitable for use as an isotype control with this antibody.</p>", "ICCIF-species-checked": "guaranteed", "ICCIF-species-dilution-info": "", "ICCIF-species-notes": "" }, "Mouse": { "FlowCyt-species-checked": "guaranteed", "FlowCyt-species-dilution-info": "", "FlowCyt-species-notes": "", "ICCIF-species-checked": "testedAndGuaranteed", "ICCIF-species-dilution-info": "1/20", "ICCIF-species-notes": "<p></p>" } } }

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Properties and storage information

Form

Liquid

Purity

Tissue culture supernatant

Storage buffer

Preservative: 0.1% Sodium azide Constituents: 5% Fetal calf serum

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

CCR9 also known as CD199 is a chemokine receptor known for its role in guiding cell traffic. It is a G-protein-coupled receptor with a mass of approximately 43 kDa. CCR9 is mainly expressed on the surface of thymocytes T lymphocytes and some subsets of dendritic cells. Its presence is especially noted in the small intestine correlated with its role in the migration and function of immune cells in the gut. This receptor is important for the homing of these cells to the intestinal mucosa engaging specifically with its ligand CCL25.

Biological function summary

CCR9 plays a significant role in the immune system particularly in the gut. It is part of chemokine receptor-ligand complexes that are integral to leukocyte trafficking. By interacting with CCL25 CCR9 facilitates the directed movement of immune cells across intestinal tissue impacting the immune surveillance and response within this environment. The receptor's expression pattern also suggests involvement in thymocyte development supporting the immune system's ability to generate a diverse repertoire of T cells.

Pathways

CCR9 is an important component of the chemokine signaling pathway. It is intricately connected to G-protein signaling events triggering downstream effects that drive cell migration and adhesion. This pathway is important for the function and maintenance of mucosal immunity linking CCR9 with CCR7 and its role in guiding T cell migration to specific tissue sites. These pathways collectively support immune functionality and homeostasis underlining CCR9's significance in tissue-specific immunity.

CCR9 has associations with inflammatory disorders like Crohn's disease and ulcerative colitis. Inflammatory bowel disease (IBD) is marked by aberrant immune cell movement and function areas where CCR9 is particularly influential. The receptor's interaction with CCL25 is pivotal in these pathological conditions often being a therapeutic target to modulate immune cell trafficking in disease management. Additionally the altered expression of CCR9 may contribute to the progression of specific T-cell leukemias further emphasizing its clinical relevance.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:

Visit the General protocols
Visit the Troubleshooting

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Target data

Receptor for chemokine SCYA25/TECK. Subsequently transduces a signal by increasing the intracellular calcium ions level.

See full target information Ccr9

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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

The Journal of experimental medicine 199:411-6 PubMed14744993

2004

Chemokine receptor CCR9 contributes to the localization of plasma cells to the small intestine.

Applications

Unspecified application

Species

Unspecified reactive species

Oliver Pabst,Lars Ohl,Meike Wendland,Marc-André Wurbel,Elisabeth Kremmer,Bernard Malissen,Reinhold Förster

View all publications

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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.

For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com

Anti-CCR9 antibody [7E7]

Flow Cytometry - Anti-CCR9 antibody [7E7] (AB52590)

Immunocytochemistry/ Immunofluorescence - Anti-CCR9 antibody [7E7] (AB52590)

Key facts

Host species

Clonality

Clone number

Isotype

Carrier free

Reacts with

Applications

Immunogen

Alternative Products

Primary Antibodies

Complementary Products

Secondary Antibodies

Primary Antibodies

Primary Antibodies

Primary Antibodies

Reactivity data

Properties and storage information

Form

Purity

Storage buffer

Shipped at conditions

Appropriate short-term storage duration

Appropriate short-term storage conditions

Appropriate long-term storage conditions

Aliquoting information

Storage information

Supplementary information

Biological function summary

Pathways

Product protocols

Target data

Publications (1)

Product promise