Anti-CCR9 antibody [HL3054] - BSA and Azide free

• IHC-P

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CCR9 antibody [HL3054] - BSA and Azide free (AB325844)

ab325844 detects CCR9 protein by immunohistochemical analysis. Sample : Paraffin-embedded human pancreatic cancer. CCR9 stained by ab325844 diluted at 1 : 100. Antigen Retrieval : Citrate buffer, pH 6.0, 15 min.

• ICC/IF

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Immunocytochemistry/ Immunofluorescence - Anti-CCR9 antibody [HL3054] - BSA and Azide free (AB325844)

ab325844 detects CCR9 protein by immunofluorescent analysis. Sample : Molt-4 cells were fixed in 4% paraformaldehyde at RT for 15 min. Green : CCR9 stained by ab325844 diluted at 1 : 100. Blue : Fluoroshield with DAPI.

• Flow Cyt

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Flow Cytometry - Anti-CCR9 antibody [HL3054] - BSA and Azide free (AB325844)

ab325844 detects CCR9 protein by flow cytometry analysis. Sample : non-fixed and non-permeabilized Molt-4 or HeLa cells were stained with 5 ug/ml (red) or a Rabbit monoclonal IgG isotype control (grey) in 1% FBS/ 5mM EDTA/PBS at 4ºC for 1 hour. Molt-4 cells staining with FcR Blocking treatment 10 mins at 4ºC.

• ICC/IF

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Immunocytochemistry/ Immunofluorescence - Anti-CCR9 antibody [HL3054] - BSA and Azide free (AB325844)

ab325844 detects CCR9 protein by immunofluorescent analysis. Sample : Mock and transfected 293T cells were fixed in ice-cold MeOH for 5 min. Green : CCR9 stained by ab325844 diluted at 1 : 500. Blue : Fluoroshield with DAPI.

• WB

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Western blot - Anti-CCR9 antibody [HL3054] - BSA and Azide free (AB325844)

Non-transfected (–) and transfected (+) boiled and unboiled 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with ab325844 diluted at 1 : 5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident femto Western HRP Substrate.

All lanes:

Western blot - Anti-CCR9 antibody [HL3054] - BSA and Azide free (ab325844) at 1/5000 dilution

Lane 1:

Non-transfected (–) boiled 293T whole cell extracts at 30 µg

Lane 2:

DDDDK-tagged CCR9 transfected (+) boiled 293T whole cell extracts at 30 µg

Lane 3:

Non-transfected (–) unboiled 293T whole cell extracts at 30 µg

Lane 4:

DDDDK-tagged CCR9 transfected (+) unboiled 293T whole cell extracts at 30 µg

Secondary

All lanes:

HRP-conjugated anti-rabbit IgG antibody

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